Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	146,404	T→C	E97E (GAA→GAG)	panD ←	aspartate 1‑decarboxylase proenzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	146,404	0	T	C	100.0%	169.6 / NA	46	E97E (GAA→GAG)	panD	aspartate 1‑decarboxylase proenzyme
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/27);  total (19/27)

GACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCTTCTTCATCTGGCATGGTAACGAAGCTGGCGATGATGACAATATCGCCGACACTGGCGCAGTGGGCCGCCGCACCGTTAACAGAAATAATTCTCGAACCGCGTTCTGCCGCGATGGCATAAGTGGAGAAACGCTTG  >  NC_000913/146271‑146539                                                                                                                                      |                                                                                                                                        gaCGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCa                                                                                                                                   <  1:410542/140‑1 (MQ=255) gaCGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGCGCGAGCCTCTTCa                                                                                                                                   >  2:246430/1‑140 (MQ=255)     aTGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTg                                                                                                                               >  2:447587/1‑140 (MQ=255)       gTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGc                                                                                                                             <  1:512636/140‑1 (MQ=255)          gcgGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATg                                                                                                                          >  2:637747/1‑140 (MQ=255)           cgGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATgg                                                                                                                         >  2:596276/1‑140 (MQ=255)              cTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATGGTaa                                                                                                                      >  2:344120/1‑140 (MQ=255)                      tAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTg                                                                                                              <  1:447587/140‑1 (MQ=255)                       aaCCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTgg                                                                                                             <  1:637747/140‑1 (MQ=255)                         ccAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTGGCg                                                                                                           <  1:737990/140‑1 (MQ=255)                           aGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTGGCgat                                                                                                         <  2:165157/140‑1 (MQ=255)                            gCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTGGCgatg                                                                                                        >  1:969614/1‑140 (MQ=255)                              cGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTGGCgatgat                                                                                                      <  2:627827/140‑1 (MQ=255)                                cAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTGGCga                                                                                                          <  2:1022086/134‑1 (MQ=255)                                cAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTGGCga                                                                                                          >  1:1022086/1‑134 (MQ=255)                                cAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTGGCGAtgatga                                                                                                    >  1:188810/1‑140 (MQ=255)                                        aCAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTGGCGATGATGACAATATCg                                                                                            <  2:1089880/140‑1 (MQ=255)                                             cAAGCAACCTGTACCGGAATCGTTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTGGCGATGATGACAATATCGCCGac                                                                                       >  1:481613/1‑140 (MQ=255)                                             cAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTGGCGATGATGACAATATCGCCGac                                                                                       <  1:1047376/140‑1 (MQ=255)                                               aGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTGGCGATGATGACAATATCGCCGacac                                                                                     >  2:570096/1‑140 (MQ=255)                                                    ccTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTGGCGATGATGACAATATCGCCGaca                                                                                      >  1:239442/1‑134 (MQ=255)                                                    ccTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTGGCGATGATGACAATATCGCCGaca                                                                                      <  2:239442/134‑1 (MQ=255)                                                    ccTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTGGCGATGATGACAATATCGCCGACACTGgcg                                                                                >  2:344837/1‑140 (MQ=255)                                                           cGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTGGCGATGATGACAATATCGCCGACACTGGCGCAGTggg                                                                         <  2:395979/140‑1 (MQ=255)                                                            ggAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTGGCGATGATGACAATATCGCCGACACTGGCGCAGTGGgc                                                                        >  2:691942/1‑140 (MQ=255)                                                              aaTCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTGGCGATGATGACAATATCGCCGACACTg                                                                                   <  2:898403/127‑1 (MQ=255)                                                              aaTCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTGGCGATGATGACAATATCGCCGACACTGGCGCAGTGGgccg                                                                      >  2:1066649/1‑140 (MQ=255)                                                              aaTCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGGCGCCAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTGGCGATGATGACAATATCGCCGACACTg                                                                                   >  1:898403/1‑127 (MQ=255)                                                                  gCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTGGCGATGATGACAATATCGCCGACACTGGCGCAGTGGGCcgccgc                                                                  <  2:188810/140‑1 (MQ=255)                                                                        gcgGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTGGCGATGATGACAATATCGCCGACACTGGCGCAGTGGGCCGCCGCACCGtt                                                            <  2:481613/140‑1 (MQ=255)                                                                        gcgGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTGGCGATGATGACAATATCGCCGACACTGGCGCAGTGGGCCGCCGCACCGtt                                                            <  1:596276/140‑1 (MQ=255)                                                                          ggTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTGGCGATGATGACAATATCGCCGACACTGGCGCAGTGGGCCGCCGCACCGTTaa                                                          <  2:294500/140‑1 (MQ=255)                                                                                      ttcattGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTGGCGATGATGACAATATCGCCGACACTGGCGCAGTGGGCCGCCGCACCGTTAACAGAAATAATtc                                              >  2:700522/1‑140 (MQ=255)                                                                                             tCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTGGCGATGATGACAATATCGCCGACACTGGCGCAGTGGGCCGCCGCACCGTTAACAGAAATAATTCTCGAAcc                                       >  2:852468/1‑140 (MQ=255)                                                                                                     aaaaTAGGCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTGGCGATGATGACAATATCGCCGACACTGGCGCAGTGGGCCGCCGCACCGTTAACAGAAATAATTCTCGAACCGCGTTCTg                               <  1:774461/140‑1 (MQ=255)                                                                                                     aaaaTAGGCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTGGCGATGATGACAATATCGCCGACACTGGCGCAGTGGGCCGCCGCACCGTTAACAGAAATAATTCTCGAACCGCGTTCTg                               <  2:950671/140‑1 (MQ=255)                                                                                                        aTAGGCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTGGCGATGATGACAATATCGCCGACACTGGCGCAGTGGGCCGCCGCACCGTTAACAGAAATAATTCTCGAACCGCGTTCTGCcg                            <  1:1066649/140‑1 (MQ=255)                                                                                                         tAGGCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTGGCGATGATGACAATATCGCCGACACTGGCGCAGTGGGCCGCCGCACCGTTAACAGAAATAATTCTCGAACCGCGTTCTGCcgc                           <  1:344120/140‑1 (MQ=255)                                                                                                          aGGCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTGGCGATGATGACAATATCGCCGACACTGGCGCAGTGGGCCGCCGCACCGTTAACAGAAATAATTCTCGAACCGCGTTCTGCcgcg                          <  1:700522/140‑1 (MQ=255)                                                                                                          aGGCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTGGCGATGATGACAATATCGCCGACACTGGCGCAGTGGGCCGCCGCACCGTTAACAGAAATAATTCTCGAACCGCGTTCTGCcgcg                          <  1:208888/140‑1 (MQ=255)                                                                                                           ggCGACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTGGCGATGATGACAATATCGCCGACACTGGCGCAGTGGGCCGCCGCACCGTTAACAGAAATAATTCTCGAACCGCGTTCTGCCGCGa                         <  2:362019/140‑1 (MQ=255)                                                                                                              gACGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTGGCGATGATGACAATATCGCCGACACTGGCGCAGTGGGCCGCCGCACCGTTAACAGAAATAATTCTCGAACCGCGTTCTGCCGCGATgg                      >  2:1115799/1‑140 (MQ=255)                                                                                                                cGTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTGGCGATGATGACAATATCGCCGACACTGGCGCAGTGGGCCGCCGCACCGTTAACAGAAATAATTCTCGAACCGCGTTCTGCCGCGATGGCa                    <  2:425039/140‑1 (MQ=255)                                                                                                                 gTTGGGTCGCCAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTGGCGATGATGACAATATCGCCGACACTGGCGCAGTGGGCCGCCGCACCGTTAACAGAAATAATTCTCGAACCGCGTTCTGCCGCGATGGCAt                   <  1:570096/140‑1 (MQ=255)                                                                                                                          ccAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTGGCGATGATGACAATATCGCCGACACTGGCGCAGTGGGCCGCCGCACCGTTAACAGAAATAATTCTCGAACCGCGTTCTGCCGCGATGGCATAAGTGgaga          >  1:253224/1‑140 (MQ=255)                                                                                                                          ccAGGTGCGAGCCTCTTCATCTGGCATGGTAACGAAGCTGGCGATGATGACAATATCGCCGACACTGGCGCAGTGGGCCGCCGCACCATTAACAGAAATAATTCTCGAACCGCGTTCTGCCGCGATGGCATAAGTGgaga          >  2:21762/1‑140 (MQ=255)                                                                                                                                  gAGCCTCTTCATCTGGCATGGTAACGAAGCTGGCGATGATGACAATATCGCCGACACTGGCGCAGTGGGCCGCCGCACCGTTAACAGAAATAATTCTCGAACCGCGTTCTGCCGCGATGGCATAAGTGGAGAAACGCTTg  <  2:969614/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        GACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCTTCTTCATCTGGCATGGTAACGAAGCTGGCGATGATGACAATATCGCCGACACTGGCGCAGTGGGCCGCCGCACCGTTAACAGAAATAATTCTCGAACCGCGTTCTGCCGCGATGGCATAAGTGGAGAAACGCTTG  >  NC_000913/146271‑146539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑