Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	724,628	C→T	V118I (GTT→ATT)	kdpC ←	K(+) transporting P‑type ATPase subunit KdpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	724,628	0	C	T	100.0%	137.7 / NA	40	V118I (GTT→ATT)	kdpC	K(+) transporting P‑type ATPase subunit KdpC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/20);  total (20/20)

TGCGATCAGTTGCGTGAGCTGTTCAACGCTGAGATTACGCGCTTTCGCCACGCGTGGGATTTGCCAGGCCGCCGCTTGCGGGGTGATATTATTGTCCAGCCCGCTTGCCGATGCCGTCACCAGTTCAACCGGAACGCTCGCGCTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCGCCAGAAGCCTGTGGATTATAG  >  NC_000913/724500‑724752                                                                                                                                 |                                                                                                                             tGCGATCAGTTGCGTGAGCTGTTCAACGCTGAGATTACGCGCTTTCGCCACGCGTGGGATTTGCCAGGCCGCCGCTTGCGGGGTGATATTATTGTCCAGCCCGCTTGCCGATGCCGTCACCAGTTCAATCGGAACGCTcg                                                                                                                   >  1:34606/1‑140 (MQ=255)        aGTTGCGTGAGCTGTTCAACGCTGAGATTACGCGCTTTCGCCACGCGTGGGATTTGCCAGGCCGCCGCTTGCGGGGTGATATTATTGTCCAGCCCGCTTGCCGATGCCGTCACCAGTTCAATCGGAACGCTCGCGCTGGc                                                                                                            >  2:688775/1‑140 (MQ=255)                  gCTGTTCAACGCTGAGATTACGCGCTTTCGCCACGCGTGGGATTTGCCAGGCCGCCGCTTGCGGGGTGATATTATTGTCCAGCCCGCTTGCCGATGCCGTCACCAGTTCAATCGGAACGCTCGCGCTGGCATCCGGGTTa                                                                                                  >  2:886717/1‑140 (MQ=255)                    tGTTCAACGCTGAGATTACGCGCTTTCGCCACGCGTGGGATTTGCCAGGCCGCCGCTTGCGGGGTGATATTATTGTCCAGCCCGCTTGCCGATGCCGTCACCAGTTCAATCGGAACGCTCGCGCTGGCATCCGGGTTAGc                                                                                                >  1:205979/1‑140 (MQ=255)                    tGTTCAACGCTGAGATTACGCGCTTTCGCCACGCGTGGGATTTGCCAGGCCGCCGCTTGCGGGGTGATATTATTGTCCAGCCCGCTTGCCGATGCCGTCACCAGTTCAATCGGAACGCTCGCGCTGGCATCCGGGTTAGc                                                                                                >  1:357363/1‑140 (MQ=255)                     gTTCAACGCTGAGATTACGCGCTTTCGCCACGCGTGGGATTTGCCAGGCCGCCGCTTGCGGGGTGATATTATTGTCCAGCCCGCTTGCCGATGCCGTCACCAGTTCAATCGGAACGCTCGCGCTGGCATCCGGGTTAGCg                                                                                               <  2:231578/140‑1 (MQ=255)                              tGAGATTACGCGCTTTCGCCACGCGTGGGATTTGCCAGGCCGCCGCTTGCGGGGTGATATTATTGTCCAGCCCGCTTGCCGATGCCGTCACCAGTTCAATCGGAACGCTCGCGCTGGCATCCGGGTTAGCGGCCCGTAAt                                                                                      >  1:86536/1‑140 (MQ=255)                                agaTTACGCGCTTTCGCCACGCGTGGGATTTGCCAGGCCGCCGCTTGCGGGGTGATATTATTGTCCAGCCCGCTTGCCGATGCCGTCACCAGTTCAATCGGAACGCTCGCGCTGGCATCCGGGTTAGCGGCCCGTAATgc                                                                                    >  1:875761/1‑140 (MQ=255)                                            ttCGCCACGCGTGGGATTTGCCAGGCCGCCGCTTGCGGGGTGATATTATTGTCCAGCCCGCTTGCCGATGCCGTCACCAGTTCAATCGGAACGCTCGCGCTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGc                                                                        <  1:23099/140‑1 (MQ=255)                                            ttCGCCACGCGTGGGATTTGCCAGGCCGCCGCTTGCGGGGTGATATTATTGTCCAGCCCGCTTGCCGATGCCGTCACCAGTTCAATCGGAACGCTCGCGCTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGc                                                                        <  2:205979/140‑1 (MQ=255)                                                      gTGGGATTTGCCAGGCCGCCGCTTGCGGGGTGATATTATTGTCCAGCCCGCTTGCCGATGCCGTCACCAGTTCAATCGGAACGCTCGCGCTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGt                                                              <  2:1023568/140‑1 (MQ=255)                                                           aTTTGCCAGGCCGCCGCTTGCGGGGTGATATTATTGTCCAGCCCGCTTGCCGATGCCGTCACCAGTTCAATCGGAACGCTCGCGCTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATc                                                         <  2:642618/140‑1 (MQ=255)                                                              tGCCAGGCCGCCGCTTGCGGGGTGATATTATTGTCCAGCCCGCTTGCCGATGCCGTCACCAGTTCAATCGGAACGCTCGCGCTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAg                                                      <  1:370865/140‑1 (MQ=255)                                                                 cAGGCCGCCGCTTGCGGGGTGATATTATTGTCCAGCCCGCTTGCCGATGCCGTCACCAGTTCAATCGGAACGCTCGCGCTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTc                                                   <  2:86536/140‑1 (MQ=255)                                                                  aGGCCGCCGCTTGCGGGGTGATATTATTGTCCAGCCCGCTTGCCGATGCCGTCACCAGTTCAATCGGAACGCTCGCGCTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCa                                                  <  2:569211/140‑1 (MQ=255)                                                                  aGGCCGCCGCTTGCGGGGTGATATTATTGTCCAGCCCGCTTGCCGATGCCGTCACCAGTTCAATCGGAACGCTCGCGCTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCa                                                  <  1:964170/140‑1 (MQ=255)                                                                    gccgccGCTTGCGGGGTGATATTATTGTCCAGCCCGCTTGCCGATGCCGTCACCAGTTCAATCGGAACGCTCGCGCTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAgg                                                >  2:554359/1‑140 (MQ=255)                                                                    gccgccGCTTGCGGGGTGATATTATTGTCCAGCCCGCTTGCCGATGCCGTCACCAGTTCAATCGGAACGCTCGCGCTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAgg                                                >  1:1027879/1‑140 (MQ=255)                                                                          gcTTGCGGGGTGATATTATTGTCCAGCCCGCTTGCCGATGCCGTCACCAGTTCAATCGGAACGCTCGCGCTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACt                                          <  1:886717/140‑1 (MQ=255)                                                                                    tGATATTATTGTCCAGCCCGCTTGCCGATGCCGTCACCAGTTCAATCGGAACGCTCGCGCTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGa                                <  2:60165/140‑1 (MQ=255)                                                                                         ttattGTCCAGCCCGCTTGCCGATGCCGTCACCAGTTCAATCGGAACGCTCGCGCTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCt                           <  1:688775/140‑1 (MQ=255)                                                                                              gTCCAGCCCGCTTGCCGATGCCGTCACCAGTTCAATCGGAACGCTCGCGCTGGCATCCGGGTTCGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTCGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCGc                      >  2:913634/1‑140 (MQ=255)                                                                                              gTCCAGCCCGCTTGCCGATGCCGTCACCAGTTCAATCGGAACGCTCGCGCTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCGc                      >  2:787650/1‑140 (MQ=255)                                                                                                 cAGCCCGCTTGCCGATGCCGTCACCAGTTCAATCGGAACGCTCGCGCTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCGCCAg                   >  1:1069729/1‑140 (MQ=255)                                                                                                  aGCCCGCTTGCCGATGCCGTCACCAGTTCAATCGGAACGCTCGCGCTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCGCCAGa                  <  2:34606/140‑1 (MQ=255)                                                                                                   gCCCGCTTGCCGATGCCGTCACCAGTTCAATCGGAACGCTCGCGCTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCGCCAGaa                 >  2:913455/1‑140 (MQ=255)                                                                                                   gCCCGCTTGCCGATGCCGTCACCAGTTCAATCGGAACGCTCGCGCTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCGCCAGaa                 >  1:160037/1‑140 (MQ=255)                                                                                                   gCCCGCTTGCCGATGCCGTCACCAGTTCAATCGGAACGCTCGCGCTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCGCCAGaa                 >  2:915267/1‑140 (MQ=255)                                                                                                    cccGCTTTCCGATGCCGTCACCAGTTCAATCGGAACGCTCGCGCTGGCATCCGGGTTAGCGGCCCGT‑ATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCGCCAGAAGc               >  2:228295/1‑140 (MQ=255)                                                                                                    cccGCTTGCCGATGCCGTCACCAGTTCAATCGGAACGCTCGCGCTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCGCCAGAAg                >  2:374044/1‑140 (MQ=255)                                                                                                    cccGCTTGCCGATGCCGTCACCAGTTCAATCGGAACGCTCGCGCTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCGCCAGAAg                >  2:122205/1‑140 (MQ=255)                                                                                                    cccGCTTGCCGATGCCGTCACCAGTTCAATCGGAACGCTCGCGCTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCGCCAGAAg                >  2:972676/1‑140 (MQ=255)                                                                                                     ccGCTTGCCGATGCCGTCACCAGTTCAATCGGAACGCTCGCGCTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCGCCAGAAGc               <  2:357363/140‑1 (MQ=255)                                                                                                     ccGCTTGCCGATGCCGTCACCAGTTCAATCGGAACGCTCGCGCTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCGCCAGAAGc               <  1:661938/140‑1 (MQ=255)                                                                                                     ccGCTTGCCGATGCCGTCACCAGTTCAATCGGAACGCTCGCGCTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCGCCAGAAGc               <  2:875761/140‑1 (MQ=255)                                                                                                     ccGCTTGCCGATGCCGTCACCAGTTCAATCGGAACGCTCGCGCTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCGCCAGAAGc               <  1:474286/140‑1 (MQ=255)                                                                                                       gCTTGCCGATGCCGTCACCAGTTCAATCGGAACGCTCGCGCTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCGCCAGAAGCCt             <  2:160037/140‑1 (MQ=255)                                                                                                         ttGCCGATGCCGTCACCAGTTCAATCGGAACGCTCGCGCTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCt                           <  1:936263/124‑1 (MQ=255)                                                                                                         ttGCCGATGCCGTCACCAGTTCAATCGGAACGCTCGCGCTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCt                           >  2:936263/1‑124 (MQ=255)                                                                                                                  ccGTCACCAGTTCAATCGGAACGCTCGCGCTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCGCCAGAAGCCTGTGGATTATAg  <  2:1069729/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                             TGCGATCAGTTGCGTGAGCTGTTCAACGCTGAGATTACGCGCTTTCGCCACGCGTGGGATTTGCCAGGCCGCCGCTTGCGGGGTGATATTATTGTCCAGCCCGCTTGCCGATGCCGTCACCAGTTCAACCGGAACGCTCGCGCTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCGCCAGAAGCCTGTGGATTATAG  >  NC_000913/724500‑724752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑