Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	782,737	A→G	Y218C (TAC→TGC)	nadA →	quinolinate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	782,737	0	A	G	100.0%	199.6 / NA	54	Y218C (TAC→TGC)	nadA	quinolinate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/40);  total (14/40)

ATCTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGACATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATACCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCTATTGTCGATATGGCGGATGCGGTCGGTTCCACCAGTCAACTGATCGCTGCTGCGAAAACATTGCCACATCAGAGGCTTATTGTGGCAACCGAT  >  NC_000913/782604‑782873                                                                                                                                      |                                                                                                                                         atcTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGACATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGa                                                                                                                                    <  1:92842/140‑1 (MQ=255) atcTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGACATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGa                                                                                                                                    <  2:962991/140‑1 (MQ=255) atcTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGACATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGa                                                                                                                                    <  2:866130/140‑1 (MQ=255) atcTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGACATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGa                                                                                                                                    <  2:616597/140‑1 (MQ=255) atcTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGACATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGa                                                                                                                                    <  2:535610/140‑1 (MQ=255) atcTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGACATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGa                                                                                                                                    <  2:470782/140‑1 (MQ=255) atcTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGACATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCAGCTTGCAAGAAGAATGCCCGGa                                                                                                                                    <  1:637583/140‑1 (MQ=255)    tGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGACATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGAtgc                                                                                                                                 <  2:662556/140‑1 (MQ=255)      ggCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGACATTCTATGCTGGCAGGGTGCCTGTATTGTGCTTGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGAtgctg                                                                                                                               <  1:187746/140‑1 (MQ=255)      ggCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGACATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGAtgctg                                                                                                                               >  2:617103/1‑140 (MQ=255)      ggCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGACATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGAtgctg                                                                                                                               <  2:332955/140‑1 (MQ=255)               cAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGACATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTgg                                                                                                                      <  1:1092410/140‑1 (MQ=255)                    aTCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGACATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCAt                                                                                                                 <  2:593075/140‑1 (MQ=255)                         gggCGTTACGTGCAAAAACAGACGGGTGGAGACATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCa                                                                                                                            >  2:1094354/1‑124 (MQ=255)                         gggCGTTACGTGCAAAAACAGACGGGTGGAGACATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCa                                                                                                                            <  1:1094354/124‑1 (MQ=255)                         gggCGTTACGTGCAAAAACAGACGGGTGGAGACATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCATCCAGa                                                                                                            >  2:565060/1‑140 (MQ=255)                         gggCGTTACGTGCAAAAACAGACGGGTGGAGACATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCATCCAGa                                                                                                            >  2:1103557/1‑140 (MQ=255)                           gCGTTACGTGCAAAAACAGACGGGTGGAGACATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCATCCAGAAt                                                                                                          <  2:202792/140‑1 (MQ=255)                               tACGTGCAAAAACAGACGGGTGGAGACATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCATCCAGAATcacc                                                                                                      <  2:320302/140‑1 (MQ=255)                                aCGTGCAAAAACAGACGGGTGGAGACATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCATCCAGAATcacca                                                                                                     <  1:1038971/140‑1 (MQ=255)                                 cGTGCAAAAACAGACGGGTGGAGACATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCATCCAGAATcaccac                                                                                                    <  1:696672/140‑1 (MQ=255)                                 cGTGCAAAAACAGACGGGTGGAGACATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCATCCAGAATcaccac                                                                                                    <  1:840726/140‑1 (MQ=255)                                    gCAAAAACAGACGGGTGGAGACATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAg                                                                                                 <  1:1024932/140‑1 (MQ=255)                                    gCAAAAACAGACGGGTGGAGACATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAg                                                                                                 <  1:282309/140‑1 (MQ=255)                                    gCAAAAACAGACGGGTGGAGACATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAg                                                                                                 <  1:377327/140‑1 (MQ=255)                                                        aCATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCTATTGTCGATATGGCGGAt                                                                             <  1:137795/140‑1 (MQ=255)                                                           ttCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCTATTGTCGATATGGCGGATGcg                                                                          >  1:535114/1‑140 (MQ=255)                                                            tCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCTATTGTCGATATGGCGGATGcgg                                                                         >  2:439262/1‑140 (MQ=255)                                                            tCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCTATTGTCGATATGGCGGATGcgg                                                                         <  1:565060/140‑1 (MQ=255)                                                                  cTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCTATTGTCGATATGGCGGATGcgg                                                                         >  1:823773/1‑134 (MQ=255)                                                                  cTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCTATTGTCGATATGGCGGATGcgg                                                                         <  2:823773/134‑1 (MQ=255)                                                                   tGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCTATTGTCGATATGGCGGATGCGGTCGGTTc                                                                  <  2:352143/140‑1 (MQ=255)                                                                   tGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCTATTGTCGATATGGCGGATGCGGTCGGTTc                                                                  <  2:108119/140‑1 (MQ=255)                                                                   tGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCTATTGTCGATATGGCGGATGCGGTCGGTTc                                                                  <  1:439262/140‑1 (MQ=255)                                                                    ggCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCTATTGTCGATATGGCGGATGCGGTCGGTTcc                                                                 >  2:711422/1‑140 (MQ=255)                                                                    ggCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCTATTGTCGATATGGCGGATGCGGTCGGTTcc                                                                 >  1:1078484/1‑140 (MQ=255)                                                                     gCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCTATTGTCGATATGGCGGATGCGGTCGGTTcca                                                                >  1:601838/1‑140 (MQ=255)                                                                     gCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCTATTGTCGATATGGCGGATGCGGTCGGTTcca                                                                >  1:882380/1‑140 (MQ=255)                                                                     gCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCTATTGTCGATATGGCGGATGCGGGCGGGTcga                                                                >  2:769237/1‑138 (MQ=255)                                                                     gCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCTATTGTCGATATGGCGGAGGCGGGCGGGTccc                                                                >  2:349206/1‑139 (MQ=255)                                                                        gggTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCTATTGTCGATATGGCGGATGCGGTCGGTTccacca                                                             <  1:1103557/140‑1 (MQ=255)                                                                                  aTTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCTATTGTCGATATGGCGGATGCGGTCGGTTCCACCAGTCAACTGAt                                                   <  2:1078484/140‑1 (MQ=255)                                                                                                      gACTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCTATTGTCGATATGGCGGATGCGGTCGGTTCCACCAGTCAACTGATCGCTGCTGCGAAAACATTGc                               <  2:882380/140‑1 (MQ=255)                                                                                                       aCTCAGGCGTTAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCTATTGTCGATATGGCGGATGCGGTCGGTTCCACCAGTCAACTGATCGCTGCTACGAAAACATTGcc                              <  1:712789/140‑1 (MQ=255)                                                                                                            ggCGTTAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCTATTGTCGATATGGCGGATGCGGTCGGTTCCACCAGTCAACTGATCGCTGCTGCGAAAACATTGCCACATc                         <  2:622908/140‑1 (MQ=255)                                                                                                             gCGTTAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCTATTGTCGATATGGCGGATGCGGTCGGTTCCACCAGTCAACTGATCGCTGCTGCGAAAACATTGCCACATCa                        <  2:535114/140‑1 (MQ=255)                                                                                                             gCGTTAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCTATTGTCGATATGGCGGATGCGGTCGGTTCCACCAGTCAACTGATCGCTGCTGCGAAAACATTGCCACATCa                        <  1:617103/140‑1 (MQ=255)                                                                                                              cGTTAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCTATTGTCGATATGGCGGATGCGGTCGGTTCCACCAGTCAACTGATCGCTGCTGCGAAAACATTGCCACATCag                       <  1:711422/140‑1 (MQ=255)                                                                                                                ttAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCTATTGTCGATATGGCGGATGCGGTCGGTTCCACCAGTCAACTGATCGCTGCTGCGAAAACATTGCCACATCagag                     <  1:592028/140‑1 (MQ=255)                                                                                                                ttAACCCGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCTATTGTCGATATGGCGGATGCGGTC‑‑TTCCACCAGTCAACTGATCGCTGCTGCGAAAACATTGCCACATCAGAGGc                   <  1:415581/140‑1 (MQ=255)                                                                                                                    cccGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCTATTGTCGATATGGCGGATGCGGTCGGTTCCACCAGTCAACTGATCGCTGCTGCGAAAACATTGCCACATCAGAGGCtt                 <  2:637838/140‑1 (MQ=255)                                                                                                                      cGCTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCTATTGTCGATATGGCGGATGCGGTCGGTTCCACCAGTCAACTGATCGCTGCTGCGAAAACATTGCCACATCAGAGGCTTAt               <  1:405929/140‑1 (MQ=255)                                                                                                                        cTTGCAAGAAGAATGCCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCTATTGTCGATATGGCGGATGCGGTCGGTTCCACCAGTCAACTGATCGCTGCTGCGAAAACATTGCCACATCAGAGGCTTAttt             >  1:1009903/1‑139 (MQ=255)                                                                                                                                   aaTGCCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCTATTGTCGATATGGCGGATGCGGTCGGTTCCACCAGTCAACTGATCGCTGCTGCGAAAACATTGCCACATCAGAGGCTTATTGTGGCAACCGAt  <  1:769237/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         ATCTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGACATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATACCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCTATTGTCGATATGGCGGATGCGGTCGGTTCCACCAGTCAACTGATCGCTGCTGCGAAAACATTGCCACATCAGAGGCTTATTGTGGCAACCGAT  >  NC_000913/782604‑782873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑