Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	794,285	A→G	R120R (CGT→CGC)	modE ←	DNA‑binding transcriptional dual regulator ModE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	794,285	0	A	G	100.0%	190.3 / NA	51	R120R (CGT→CGC)	modE	DNA‑binding transcriptional dual regulator ModE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/27);  total (24/27)

GCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCGGGTCAGTACTGCG  >  NC_000913/794154‑794420                                                                                                                                    |                                                                                                                                        gCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGAt                                                                                                                                 <  2:32202/140‑1 (MQ=255)         tGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCca                                                                                                                         <  2:733856/140‑1 (MQ=255)              cTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAgg                                                                                                                    <  2:493956/140‑1 (MQ=255)               tttCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGc                                                                                                                   <  1:485542/140‑1 (MQ=255)                 tCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTg                                                                                                                 >  2:272136/1‑140 (MQ=255)                   aGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGtt                                                                                                               <  2:552103/140‑1 (MQ=255)                                    aGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCAt                                                                                              <  2:988985/140‑1 (MQ=255)                                    aGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCAt                                                                                              <  2:34877/140‑1 (MQ=255)                                    aGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCAt                                                                                              <  1:184665/140‑1 (MQ=255)                                      ccAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCg                                                                                            >  1:389563/1‑140 (MQ=255)                                         gTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCa                                                                                         <  2:996072/140‑1 (MQ=255)                                               cATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCAt                                                                                              >  1:125666/1‑129 (MQ=255)                                               cATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCAt                                                                                              <  2:125666/129‑1 (MQ=255)                                                        gCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAg                                                                          >  1:165244/1‑140 (MQ=255)                                                        gCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAg                                                                          >  1:687038/1‑140 (MQ=255)                                                        gCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAg                                                                          >  1:118464/1‑140 (MQ=255)                                                           gTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCt                                                                       <  1:666064/140‑1 (MQ=255)                                                            ttGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCtt                                                                      >  2:83033/1‑140 (MQ=255)                                                             tGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCttt                                                                     >  1:31460/1‑140 (MQ=255)                                                                    catcatGATCGCGGGCGGTGATGGTACCGAACCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTgg                                                              >  2:406092/1‑140 (MQ=255)                                                                    catcatGATCGCGGGCGGTGATGGTACCGAACCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTgg                                                              >  2:301322/1‑140 (MQ=255)                                                                    catcatGATCGCGGGCGGTGATGGTACCGAACCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTgg                                                              >  1:908257/1‑140 (MQ=255)                                                                        atGATCGCGGGCGGTGATGGTACCGAACCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGAttt                                                          <  2:488814/140‑1 (MQ=255)                                                                            tCGCGGGCGGTGATGGTACCGAACCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTgcgc                                                      <  1:301322/140‑1 (MQ=255)                                                                              gcgggcggTGATGGTACCGAACCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCa                                                    >  2:148831/1‑140 (MQ=255)                                                                              gcgggcggTGATGGTACCGAACCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCa                                                    <  2:31460/140‑1 (MQ=255)                                                                               cgggcggTGATGGTACCGAACCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAg                                                   <  2:118464/140‑1 (MQ=255)                                                                                 ggcggTGATGGTACCGAACCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTa                                                 <  1:322200/140‑1 (MQ=255)                                                                                 ggcggTGATGGTACCGAACCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTa                                                 <  2:889323/140‑1 (MQ=255)                                                                                  gcggTGATGGTACCGAACCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTaa                                                >  2:442873/1‑140 (MQ=255)                                                                                      tGATGGTACCGAACCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCa                                            <  2:439832/140‑1 (MQ=255)                                                                                         tGGTACCGAACCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATag                                         <  1:442873/140‑1 (MQ=255)                                                                                                gAACCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGaa                                  >  1:377133/1‑140 (MQ=255)                                                                                                gAACCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGaa                                  >  2:320570/1‑140 (MQ=255)                                                                                                  aCCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATc                                >  2:946603/1‑140 (MQ=255)                                                                                                  aCCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATc                                >  1:874065/1‑140 (MQ=255)                                                                                                      cTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTc                            <  2:377133/140‑1 (MQ=255)                                                                                                      cTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTc                            >  2:928161/1‑140 (MQ=255)                                                                                                      cTGGTTACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTc                            >  2:51900/1‑140 (MQ=255)                                                                                                      cTGGATACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCTGCCAGCAGGCTGTTCAGCGGCACGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTc                            >  2:1102828/1‑140 (MQ=255)                                                                                                          ttACGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTg                        >  2:570228/1‑140 (MQ=255)                                                                                                            aCGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGAc                      <  1:391035/140‑1 (MQ=255)                                                                                                            aCGGGCGCTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGAc                      <  2:908257/140‑1 (MQ=255)                                                                                                                 cgcTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAg                 <  1:144699/140‑1 (MQ=255)                                                                                                                 cgcTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAg                 <  1:928161/140‑1 (MQ=255)                                                                                                                 cgcTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAg                 <  1:1121401/140‑1 (MQ=255)                                                                                                                 cgcTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAg                 <  1:272136/140‑1 (MQ=255)                                                                                                                 cgcTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCAAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAg                 <  2:389563/140‑1 (MQ=255)                                                                                                                   cTGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAAGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCg               >  1:346880/1‑140 (MQ=255)                                                                                                                    tGGTTTGCAGTGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCgg              >  1:1009911/1‑140 (MQ=255)                                                                                                                              tGAAAAGCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCGGGTCAGTACTg    >  2:729978/1‑140 (MQ=255)                                                                                                                                aaaagCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCGGGTCAGTACTgcg  >  2:771961/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                        GCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCGGGTCAGTACTGCG  >  NC_000913/794154‑794420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑