Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 885,736 A→G R132R (CGT→CGC)  ybjI ← 5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913885,7360AG100.0% 230.2 / NA 61R132R (CGT→CGC) ybjI5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/30);  total (31/30)

TGTGGACCGACACCATAATATCGCCGATGGCCTCATGTAATGCTTTTTGTACTTGTGGAATCAGTTCATCGGAAAGATTCAGGCCAAACTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGACGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGAGTGTATAGGCACTATTTTTTCCGCAGGCAATAATTTCCACTTCCGGGCGCGTCAGCAAATGTTCCACGACAGTAGCAAACGCA  >  NC_000913/885600‑885871
                                                                                                                                        |                                                                                                                                       
tgtgGACCGACACCATAATATCGCCGATGGCCTCATGTAATGCTTTTTGTACTTGTGGAATCAGTTCATCGGAAAGATTCAGGCCAAACTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCgg                                                                                                                                      >  2:1112204/1‑140 (MQ=255)
  tgGACCGACACCATAATATCGCCGATGGCCTCATGTAATGCTTTTTGTACTTGTGGAATCAGTTCATCGGAAAGATTCAGGCCAAACTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTg                                                                                                                                    >  1:643891/1‑140 (MQ=255)
   ggACCGACACCATAATATCGCCGATGGCCTCATGTAATGCTTTTTGTACTTGTGGAATCAGTTCATCGGAAAGATTCAGGCCAAACTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGa                                                                                                                                   <  1:513397/140‑1 (MQ=255)
    gACCGACACCATAATATCGCCGATGGCCTCATGTAATGCTTTTTGTACTTGTGGAATCAGTTCATCGGAAAGATTCAGGCCAAACTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGat                                                                                                                                  >  1:634402/1‑140 (MQ=255)
      ccGACACCATAATATCGCCGATGGCCTCATGTAATGCTTTTTGTACTTGTGGAATCAGTTCATCGGAAAGATTCAGGCCAAACTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGataa                                                                                                                                <  1:286572/140‑1 (MQ=255)
                aataTCGCCGATGGCCTCATGTAATGCTTTTTGTACTTGTGGAATCAGTTCATCGGAAAGATTCAGGCCAAACTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCg                                                                                                                      <  2:827209/140‑1 (MQ=255)
                 atatCGCCGATGGCCTCATGTAATGCTTTTTGTACTTGTGGAATCAGTTCATCGGAAAGATTCAGGCCAAACTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGc                                                                                                                     <  1:57654/140‑1 (MQ=255)
                  tatCGCCGATGGCCTCATGTAATGCTTTTTGTACTTGTGGAATCAGTTCATCGGAAAGATTCAGGCCAAACTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGcc                                                                                                                    <  1:108258/140‑1 (MQ=255)
                   atCGCCGATGGCCTCATGTAATGCTTTTTGTACTTGTGGAATCAGTTCATCGGAAAGATTCAGGCCAAACTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCa                                                                                                                   <  2:746442/140‑1 (MQ=255)
                     cGCCGATGGCCTCATGTAATGCTTTTTGTACTTGTGGAATCAGTTCATCGGAAAGATTCAGGCCAAACTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCAcc                                                                                                                 <  2:924538/140‑1 (MQ=255)
                     cGCCGATGGCCTCATGTAATGCTTTTTGTACTTGTGGAATCAGTTCATCGGAAAGATTCAGGCCAAACTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCAcc                                                                                                                 >  1:908950/1‑140 (MQ=255)
                     cGCCGATGGCCTCATGTAATGCTTTTTGTACTTGTGGAATCAGTTCATCGGAAAGATTCAGGCCAAACTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCAcc                                                                                                                 >  1:924538/1‑140 (MQ=255)
                       ccGATGGCCTCATGTAATGCTTTTTGTACTTGTGGAATCAGTTCATCGGAAAGATTCAGGCCAAACTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGt                                                                                                               >  1:431703/1‑140 (MQ=255)
                       ccGATGGCCTCATGTAATGCTTTTTGTACTTGTGGAATCAGTTCATCGGAAAGATTCAGGCCAAACTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGt                                                                                                               >  2:1105117/1‑140 (MQ=255)
                       ccGATGGCCTCATGTAATGCTTTTTGTACTTGTGGAATCAGTTCATCGGAAAGATTCAGGCCAAACTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGt                                                                                                               >  1:640073/1‑140 (MQ=255)
                               cTCATGTAATGCTTTTTGTACTTGTGGAATCAGTTCATCGGAAAGATTCAGGCCAAACTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATgg                                                                                                       <  2:634402/140‑1 (MQ=255)
                                   tGTAATGCTTTTTGTACTTGTGGAATCAGTTCATCGGAAAGATTCAGGCCAAACTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATc                                                                                                   <  1:238530/140‑1 (MQ=255)
                                     tAATGCTTTTTGTACTTGTGGAATCAGTTCATCGGAAAGATTCAGGCCAAACTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGt                                                                                                 <  2:431703/140‑1 (MQ=255)
                                          cTTTTTGTACTTGTGGAATCAGTTCATCGGAAAGATTCAGGCCAAACTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCatat                                                                                            <  2:886272/140‑1 (MQ=255)
                                             tttGTACTTGTGGAATCAGTTCATCGGAAAGATTCAGGCCAAACTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATAtttt                                                                                         >  1:533822/1‑140 (MQ=255)
                                             tttGTACTTGTGGAATCAGTTCATCGGAAAGATTCAGGCCAAACTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATAtttt                                                                                         >  2:722116/1‑140 (MQ=255)
                                               tGTACTTGTGGAATCAGTTCATCGGAAAGATTCAGGCCAAACTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATAtttttt                                                                                       <  1:955952/140‑1 (MQ=255)
                                                 tACTTGTGGAATCAGTTCATCGGAAAGATTCAGGCCAAACTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGa                                                                                     <  1:1112204/140‑1 (MQ=255)
                                                     tgtgGAATCAGTTCATCGGAAAGATTCAGGCCAAACTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCACCACCGTTTTCATGGCATCGTCATATTTTTTGAgtgt                                                                                 >  1:337626/1‑140 (MQ=255)
                                                         gAATCAGTTCATCGGAAAGATTCAGGCCAAACTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGAGTGTATAg                                                                             >  1:274713/1‑140 (MQ=255)
                                                              aGTTCATCGGAAAGATTCAGGCCAAACTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGAg                                                                                    <  2:1051778/128‑1 (MQ=255)
                                                              aGTTCATCGGAAAGATTCAGGCCAAACTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGAg                                                                                    >  1:1051778/1‑128 (MQ=255)
                                                              aGTTCATCGGAAAGATTCAGGCCAAACTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGAGTGTATAGGCACt                                                                        >  2:364016/1‑140 (MQ=255)
                                                              aGTTCATCGGAAAGATTCAGGCCAAACTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGAGTGTATAGGCACt                                                                        >  1:744929/1‑140 (MQ=255)
                                                                ttCATCGGAAAGATTCAGGCCAAACTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGAGTGTATAGGCACTAt                                                                      >  2:24212/1‑140 (MQ=255)
                                                                     cGGAAAGATTCAGGCCAAACTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGAGTGTATAGGCACTAtttttt                                                                 >  2:299412/1‑140 (MQ=255)
                                                                          aGATTCAGGCCAAACTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGAGTGTATAGGCACTATTTTTTCCGCa                                                            <  1:1105117/140‑1 (MQ=255)
                                                                          aGATTCAGGCCAAACTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGAGTGTATAGGCACTATTTTTTCCGCa                                                            <  2:274713/140‑1 (MQ=255)
                                                                              tCAGGCCAAACTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGAGTGTATAGGCACTATTTTTTCCGCAGGCa                                                        <  2:984378/140‑1 (MQ=255)
                                                                                   ccAAACTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGAGTGTATAGGCACTATTTTTTCCGCAGGCaataat                                                   <  1:24212/140‑1 (MQ=255)
                                                                                     aaaCTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGAGTGTATAGGCACTATTTTTTCCGCAGGCAATAAttt                                                 <  2:643891/140‑1 (MQ=255)
                                                                                      aaCTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGAGTGTATAGGCACTATTTTTTCCGCAGGCAATAATTTc                                                >  2:1059613/1‑140 (MQ=255)
                                                                                            aagaagATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGAGTGTATAGGCACTATTTTTTCCGCAGGCAATAATTTCCACTTc                                          >  1:1042714/1‑140 (MQ=255)
                                                                                                agaTATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCACCACCGTTTTCATGGCATCGTCATATTTTTTGAGTGTATAGGCACTATTTTTTCCGCAGACAATAATTTCCACTTCCggg                                      <  2:337626/140‑1 (MQ=255)
                                                                                                   tatCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGAGTGTATAGGCACTATTTTTTCCGCAGGCAATAATTTCCACTTCCGGgcgc                                   >  2:636321/1‑140 (MQ=255)
                                                                                                     tCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGAGTGTATAGGCACTATTTTTTCCGCAGGCAATAATTTCCACTTCCGGGCGCGt                                 >  1:515539/1‑140 (MQ=255)
                                                                                                            aGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGAGTGTATAGGCACTATTTTTTCCGCAGGCAATAATTTCCACTTCCGGGCGCGTCAGCaaa                          <  2:744929/140‑1 (MQ=255)
                                                                                                            aGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGAGTGTATAGGCACTATTTTTTCCGCAGGCAATAATTTCCACTTCCGGGCGCGTCAGCaaa                          <  1:1059613/140‑1 (MQ=255)
                                                                                                            aGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGAGTGTATAGGCACTATTTTTTCCGCAGGCAATAATTTCCACTTCCGGGCGCGTCAGCaaa                          >  1:934798/1‑140 (MQ=255)
                                                                                                            aGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGAGTGTATAGGCACTATTTTTTCCGCAGGCAATAATTTCCACTTCCGGGCGCGTCAGCaaa                          <  2:1042714/140‑1 (MQ=255)
                                                                                                            aGTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGAGTGTATAGGCACTATTTTTTCCGCAGGCAATAATTTCCACTTCCGGGCGCGTCAGCaaa                          <  2:934798/140‑1 (MQ=255)
                                                                                                             gTTGTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGAGTGTATAGGCACTATTTTTTCCGCAGGCAATAATTTCCACTTCCGGGCGCGTCAGCAAAt                         >  2:900455/1‑140 (MQ=255)
                                                                                                                gTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGAGTGTATAGGCACTATTTTTTCCGCAGGCAATAATTTCCACTTCCGGGCGCGTCAGCAAATGtt                      >  2:941239/1‑140 (MQ=255)
                                                                                                                gTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGAGTGTATAGGCACTATTTTTTCCGCAGGCAATAATTTCCACTTCCGGGCGCGTCAGCAAATGtt                      >  1:621131/1‑140 (MQ=255)
                                                                                                                gTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGAGTGTATAGGCACTATTTTTTCCGCAGGCAATAATTTCCACTTCCGGGCGCGTCAGCAAATGtt                      >  1:24270/1‑140 (MQ=255)
                                                                                                                gTCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGAGTGGATAGGCACTATTTTTTCCGCAGGCAATAATTTCCACTTCCGGGCGCGTCAGCAAATGtt                      >  2:470238/1‑140 (MQ=255)
                                                                                                                 tCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGAGTGTATAGGCACTATTTTTTCCGCAGGCAATAATTTCCACTTCCGGGCGCGTCAGCAAATGTTc                     >  1:295602/1‑140 (MQ=255)
                                                                                                                 tCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGAGTGTATAGGCACTATTTTTTCCGCAGGCAATAATTTCCACTTCCGGGCGCGTCAGCAAATGTTc                     <  2:515539/140‑1 (MQ=255)
                                                                                                                 tCAAAGTTATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGAGTGTATAGGCACTATTTTTTCCGCAGGCAATAATTTCCACTTCCGGGCGCGTCAGCAAATGTTc                     >  1:801450/1‑140 (MQ=255)
                                                                                                                       ttATCGACGTATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGAGTGTATAGGCACTATTTTTTCCGCAGGCAATAATTTCCACTTCCGGGCGCGTCAGCAAATGTTCCACGAc               >  1:789517/1‑140 (MQ=255)
                                                                                                                                tATTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGAGTGTATAGGCACTATTTTTTCCGCAGGCAATAATTTCCACTTCCGGGCGCGTCAGCAAATGTTCCACGACAGTAGCaaa      <  1:364016/140‑1 (MQ=255)
                                                                                                                                 aTTCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGAGTGTATAGGCACTATTTTTTCCGCAGGCAATAATTTCCACTTCCGGGCGCGTCAGCAAATGTTCCACGACAGTAGCAAAc     >  2:1106144/1‑140 (MQ=255)
                                                                                                                                  ttCCAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGAGTGTATAGGCACTATTTTTTCCGCAGGCAATAATTTCCACTTCCGGGCGCGTCAGCAAATGTTCCACGACAGTAGCAAACg    <  2:789517/140‑1 (MQ=255)
                                                                                                                                    ccAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGAGTGTATAGGCACTATTTTTTCCGCAGGCAATAATTTCCACTTCCGGGCGCGTCAGCAAATGTTCCACGACAGTAGCAAACGCa  <  1:275177/140‑1 (MQ=255)
                                                                                                                                    ccAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGAGTGTATAGGCACTATTTTTTCCGCAGGCAATAATTTCCACTTCCGGGCGCGTCAGCAAATGTTCCACGACAGTAGCAAACGCa  <  2:192815/140‑1 (MQ=255)
                                                                                                                                    ccAGGCGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGAGTGTATAGGCACTATTTTTTCCGCAGGCAATAATTTCCACTTCCGGGCGCGTCAGCAAATGTTCCACGACAGTAGCAAACGCa  <  2:1051371/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                       
TGTGGACCGACACCATAATATCGCCGATGGCCTCATGTAATGCTTTTTGTACTTGTGGAATCAGTTCATCGGAAAGATTCAGGCCAAACTTAAAGAAGATATCCTCTAAGTTGTCAAAGTTATCGACGTATTCCAGACGGTGATAATACATTTCCGCCACCGTTTTCATGGCATCGTCATATTTTTTGAGTGTATAGGCACTATTTTTTCCGCAGGCAATAATTTCCACTTCCGGGCGCGTCAGCAAATGTTCCACGACAGTAGCAAACGCA  >  NC_000913/885600‑885871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑