Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	937,408	A→G	S13G (AGC→GGC)	lolA →	outer membrane lipoprotein carrier protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	937,408	0	A	G	100.0%	186.3 / NA	50	S13G (AGC→GGC)	lolA	outer membrane lipoprotein carrier protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/22);  total (28/22)

AAATTCAGTATTTTCTTCTTTCCTCAAGCTGATTATTAGCCTGGAATAGAGAGTAGAGGGAACTCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAAGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAGCGATCTGAAAAGCCGCCTGGATAAAGTCAGCAGCTTCCACGCCAGCTTCACACAAAAAGTGACTGACGGTAGCGGCGCGGCGGTGCAGGAAGGTCAGGG  >  NC_000913/937274‑937544                                                                                                                                       |                                                                                                                                         aaaTTCAGTATTTTCTTCTTTCCTCAAGCTGATTATTAGCCTGGAATAGAGAGTAGAGGGAACTCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAGGCTta                                                                                                                                     >  2:1118851/1‑140 (MQ=255) aaaTTCAGTATTTTCTTCTTTCCTCAAGCTGATTATTAGCCTGGAATAGAGAGTAGAGGGAACTCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAGGCTta                                                                                                                                     <  2:476349/140‑1 (MQ=255)         tATTTTCTTCTTTCCTCAAGCTGATTATTAGCCTGGAATAGAGAGTAGAGGGAACTCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAGGCTTAGTagcaag                                                                                                                             <  1:864820/140‑1 (MQ=255)          aTTTTCTTCTTTCCTCAAGCTGATTATTAGCCTGGAATAGAGAGTAGAGGGAACTCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAGGCTTAGTagcaagc                                                                                                                            <  1:1016545/140‑1 (MQ=255)          aTTTTCTTCTTTCCTCAAGCTGATTATTAGCCTGGAATAGAGAGTAGAGGGAACTCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAGGCTTAGTagcaagc                                                                                                                            <  1:190225/140‑1 (MQ=255)                ttcttTCCTCAAGCTGATTATTAGCCTGGAATAGAGAGTAGAGGGAACTCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAGGCTTAGTAGCAAGCAGCGtt                                                                                                                      <  1:664328/140‑1 (MQ=255)                 tcttTCCTCAAGCTGATTATTAGCCTGGAATAGAGAGTAGAGGGAACTCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAGGCTTAGTAGCAAGCAGCGttt                                                                                                                     >  2:114976/1‑140 (MQ=255)                    ttCCTCAAGCTGATTATTAGCCTGGAATAGAGAGTAGAGGGAACTCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAGGCTTAGTAGCAAGCAGCGTTTggg                                                                                                                  <  1:708946/140‑1 (MQ=255)                    ttCCTCAAGCTGATTATTAGCCTGGAATAGAGAGTAGAGGGAACTCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAGGCTTAGTAGCAAGCAGCGTTTggg                                                                                                                  <  1:829494/140‑1 (MQ=255)                          aaGCTGATTATTAGCCTGGAATAGAGAGTAGAGGGAACTCCCGATCGGGAGTGACGTAATTTGAGGAATAACGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATg                                                                                                            >  2:280766/1‑140 (MQ=255)                                    ttaGCCTGGAATAGAGAGTAGAGGGAACTCCCGATCGGGAGTGACGTAATTTGAGGAATAACGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAGCGa                                                                                                  <  1:280766/140‑1 (MQ=255)                                      aGCCTGGAATAGAGAGTAGAGGGAACTCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGc                                                                                                           <  1:445728/129‑1 (MQ=255)                                      aGCCTGGAATAGAGAGTAGAGGGAACTCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGc                                                                                                           >  2:445728/1‑129 (MQ=255)                                            gAATAGAGAGTAGAGGGAACTCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAGCGATCTGaaaa                                                                                          >  1:768484/1‑140 (MQ=255)                                                 gagagTAGAGGGAACTCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAg                                                                                                     <  2:605096/124‑1 (MQ=255)                                                 gagagTAGAGGGAACTCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAg                                                                                                     >  1:605096/1‑124 (MQ=255)                                                  agagTAGAGGGAACTCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAGCGATCTGAAAAgccgcc                                                                                    >  1:341161/1‑140 (MQ=255)                                                      tAGAGGGAACTCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAGCGATCTGaaaa                                                                                          <  1:384100/130‑1 (MQ=255)                                                      tAGAGGGAACTCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAGCGATCTGaaaa                                                                                          >  2:384100/1‑130 (MQ=255)                                                      tAGAGGGAACTCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAGCGATCTGAAAAGCCGCCTGGa                                                                                >  2:765315/1‑140 (MQ=255)                                                       agagGGAACTCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAGCGATCTGAAAAGCCGCCTGGAt                                                                               >  2:901781/1‑140 (MQ=255)                                                         agGGAACTCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAGCGATCTGAAAAGCCGCCTGGATaa                                                                             >  1:45645/1‑140 (MQ=255)                                                              agtCCCGGTCGGGAGTGACGTAATTTGAGGAATAGTGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAGGCTTAGTAGCAAGCAGCGTTTCGGCTGATGCAGCAAGCGATCTGAAAAGCCGCCTCGATAAAGCca                                                                        >  2:750142/3‑140 (MQ=255)                                                                tCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAGCGATCTGAAAAGCCGCCTGGATAAAGTcagc                                                                      >  2:1074264/1‑140 (MQ=255)                                                                tCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAGCGATCTGAAAAGCCGCCTGGATAAAGTcagc                                                                      >  2:701502/1‑140 (MQ=255)                                                                   cGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAGCGATCTGAAAAGCCGCCTGGATAAAGTCagcagc                                                                   >  2:11582/1‑140 (MQ=255)                                                                         ggAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAGCGATCTGAAAAGCCGCCTGGATAAAGTCAGCAGCTTCCAc                                                             <  1:114976/140‑1 (MQ=255)                                                                                cGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAGCGATCTGAAAAGCCGCCTGGATAAAGTCAGCAGCTTCCACGCCAGCt                                                      >  1:459431/1‑140 (MQ=255)                                                                                 gTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAGGCTTAGTAGTAAGCAGCGTTTGGGCTGATGCCGCAAGCGATCTGAAAAGCCGCTTGGATAAAGGCAGCAGCTACCACGCCAGCtt                                                     <  2:1093122/140‑1 (MQ=255)                                                                                  tAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAGCGATCTGAAAAGCCGCCTGGATAAAGTCAGCAGCTTc                                                                >  2:623680/1‑128 (MQ=255)                                                                                  tAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAGCGATCTGAAAAGCCGCCTGGATAAAGTCAGCAGCTTc                                                                <  1:623680/128‑1 (MQ=255)                                                                                         aGGAATAATGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAGCGATCTGAAAAGCCGCCTGGATAAAGTCAGCAGCTTCCACGGCAGCTTCACACaaa                                             >  2:695431/1‑140 (MQ=255)                                                                                         aGGAATAATGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAGCGATCTGAAAAGCCGCCTGGATAAAGTCAGCAGCTTCCACGCCAGCTTCACACaaa                                             >  1:872919/1‑140 (MQ=255)                                                                                            aataatGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAGCGATCTGAAAAGCCGCCTGGATAAAGTCAGCAGCTTCCACGCCAGCTTCACACAAAAAg                                          <  2:872919/140‑1 (MQ=255)                                                                                            aataatGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAGCGATCTGAAAAGCCGCCTGGATAAAGTCAGCAGCTTCCACGCCAGCTTCACACAAAAAg                                          <  1:765315/140‑1 (MQ=255)                                                                                             ataatGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAGCGATCTGAAAAGCCGCCTGGATAAAGTCAGCAGCTTCCACGCCAGCTTCACACAAAAAGt                                         >  2:416704/1‑140 (MQ=255)                                                                                               aatGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAGCGATCTGAAAAGCCGCCTGGATAAAGTCAGCAGCTTCCACGCCAGCTTCACACAAAAAGtga                                       <  1:11582/140‑1 (MQ=255)                                                                                               aatGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAGCGATCTGAAAAGCCGCCTGGATAAAGTCAGCAGCTTCCACGCCAGCTTCACACAAAAAGtga                                       <  1:1074264/140‑1 (MQ=255)                                                                                                      aaaaaaaTTGCCATCACCTGTGCATTACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAGCGATCTGAAAAGCCGCCTGGATAAAGTCAGCAGCTTCCACGCCAGCTTCACACAAAAAGTGACTGACgg                                >  1:35002/1‑140 (MQ=255)                                                                                                             ttGCCATCACCTGTGCATTACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAGCGATCTGAAAAGCCGCCTGGATAAAGTCAGCAGCTTCCACGCCAGCTTCACACAAAAAGTGACTGACGGTAgcggc                         >  2:266270/1‑140 (MQ=255)                                                                                                                 cATCACCTGTGCATTACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAGCGATCTGAAAAGCCGCCTGGATAAAGTCAGCAGCTTCCACGCCAGCTTCACACAAAAAGTGACTGACGGTAgcggcgcgg                     <  2:341161/140‑1 (MQ=255)                                                                                                                          tgCATTACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAGCGATCTGAAAAGCCGCCTGGATAAAGTCAGCAGCTTCCACGCCAGCTTCACACAAAAAGTGACTGACGGTAGCGGCGCGGCGGTGCAgg            >  2:613071/1‑140 (MQ=255)                                                                                                                               tACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAGCGATCTGAAAAGCCGCCTGGATAAAGTCAGCAGCTTCCACGCCAGCTTCACACAAAAAGTGACTGACGGTAgcggcgcgg                     <  2:900023/126‑1 (MQ=255)                                                                                                                               tACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAGCGATCTGAAAAGCCGCCTGGATAAAGTCAGCAGCTTCCACGCCAGCTTCACACAAAAAGTGACTGACGGTAgcggcgcgg                     >  1:900023/1‑126 (MQ=255)                                                                                                                               tACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAGCGATCTGAAAAGCCGCCTGGATAAAGTCAGCAGCTTCCACGCCAGCTTCACACAAAAAGTGACTGACGGTAgcggcgcgg                     >  1:745573/1‑126 (MQ=255)                                                                                                                               tACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAGCGATCTGAAAAGCCGCCTGGATAAAGTCAGCAGCTTCCACGCCAGCTTCACACAAAAAGTGACTGACGGTAgcggcgcgg                     <  2:745573/126‑1 (MQ=255)                                                                                                                               tACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAGCGATCTGAAAAGCCGCCTGGATAAAGTCAGCAGCTTCCACGCCAGCTTCACACAAAAAGTGACTGACGGTAGCGGCGCGGCGGTGCAgg            >  1:1054390/1‑135 (MQ=255)                                                                                                                               tACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAGCGATCTGAAAAGCCGCCTGGATAAAGTCAGCAGCTTCCACGCCAGCTTCACACAAAAAGTGACTGACGGTAGCGGCGCGGCGGTGCAgg            <  2:1054390/135‑1 (MQ=255)                                                                                                                               tACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAGCGATCTGAAAAGCCGCCTGGATAAAGTCAGCAGCTTCCACGCCAGCTTCACACAAAAAGTGACTGACGGTAGCGGCGCGGCGGTGCAGGAAggg       >  2:285901/1‑139 (MQ=255)                                                                                                                               tACTCTCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAGCGATCTGAAAAGCCGCCTGGATAAAGTCAGCAGCTTCCACGCCAGCTTCACACAAAAAGTGACTGACGGTAGCGGCGCGGCGGTGCAGGAAGGt       >  1:847911/1‑140 (MQ=255)                                                                                                                                 ctctCAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAGCGATCTGAAAAGCCGCCTGGATAAAGTCAGCAGCTTCCACGCCAGCTTCACACAAAAAGTGACTGACGGTAGCGGCGCGGCGGTGCAGGAAGGTCa     >  2:1067899/1‑140 (MQ=255)                                                                                                                                    tcAGGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAGCGATCTGAAAAGCCGCCTGGATAAAGTCAGCAGCTTCCACGCCAGCTTCACACAAAAAGTGACTGACGGTAGCGGCGCGGCGGTGCAGGAAGGTCAggg  <  2:459431/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         AAATTCAGTATTTTCTTCTTTCCTCAAGCTGATTATTAGCCTGGAATAGAGAGTAGAGGGAACTCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGCATTACTCTCAAGCTTAGTAGCAAGCAGCGTTTGGGCTGATGCCGCAAGCGATCTGAAAAGCCGCCTGGATAAAGTCAGCAGCTTCCACGCCAGCTTCACACAAAAAGTGACTGACGGTAGCGGCGCGGCGGTGCAGGAAGGTCAGGG  >  NC_000913/937274‑937544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑