Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	959,171	T→C	R120R (CGT→CGC)	aroA →	3‑phosphoshikimate 1‑carboxyvinyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	959,171	0	T	C	100.0%	165.6 / NA	45	R120R (CGT→CGC)	aroA	3‑phosphoshikimate 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/24);  total (21/24)

TATCGGTAACGGCGGTCCATTACACGCAGAAGGTGCCCTGGAGTTGTTCCTCGGTAACGCCGGAACGGCAATGCGTCCGCTGGCGGCAGCTCTTTGTCTGGGTAGCAATGATATTGTGCTGACCGGTGAGCCGCGTATGAAAGAACGCCCGATTGGTCATCTGGTGGATGCGCTGCGCCTGGGCGGGGCGAAGATCACTTACCTGGAACAAGAAAATTATCCGCCGTTGCGTTTACAGGGCGGCTTTACTGGCGGCAACGTTGACGTTGAT  >  NC_000913/959036‑959306                                                                                                                                        |                                                                                                                                        tATCGGTAACGGCGGTCCATTACACGCAGAAGGTGCCCTGGAGTTGTTCCTCGGTAACGCCGGAACGGCAATGCGTCCGCTGGCGGCAGCTCTTTGTCTGGGTAGCAATGATATTGTGCTGACCGGTGAGCCGCGCATGa                                                                                                                                     >  1:978945/1‑140 (MQ=255)     ggTAACGGCGGTCCATTACACGCAGAAGGTGCCCTGGAGTTGTTCCTCGGTAACGCCGGAACGGCAATGCGTCCGCTGGCGGCAGCTCTTTGTCTGGGTAGCAATGATATTGTGCTGACCGGTGAGCCGCGCATGAAAGa                                                                                                                                 <  2:573616/140‑1 (MQ=255)      gTAACGGCGGTCCATTACACGCAGAAGGTGCCCTGGAGTTGTTCCTCGGTAACGCCGGAACGGCAATGCGTCCGCTGGCGGCAGCTCTTTGTCTGGGTAGCAATGATATTGTGCTGACCGGTGAGCCGCGCATGAAAGaa                                                                                                                                <  1:84107/140‑1 (MQ=255)        aaCGGCGGTCCATTACACGCAGAAGGTGCCCTGGAGTTGTTCCTCGGTCACGCCGGAACGGCAATGCGTCCGCTGGCGGCAGCTCTTTGTCTGGGTAGCAATGATATTGTGCTGACCGGTGAGCCGCGCATGAAAGAACg                                                                                                                              >  2:911323/1‑140 (MQ=255)              ggTCCATTACACGCAGAAGGTGCCCTGGAGTTGTTCCTCGGTAACGCCGGAACGGCAATGCGTCCGCTGGCGGCAGCTCTTTGTCTGGGTAGCAATGATATTGTGCTGACCGGTGAGCCGCGCATGAAAGAACGCCCGAt                                                                                                                        <  1:1086225/140‑1 (MQ=255)              ggTCCATTACACGCAGAAGGTGCCCTGGAGTTGTTCCTCGGTAACGCCGGAACCGCAATGCGTCCGCTGGCGGCAGCTCTTTGTCTGGGTAGCAATGATATTGTGCTGACCGGTGAGCCGCGCATGAAAGAACGCCCGAt                                                                                                                        <  2:703666/140‑1 (MQ=255)               gTCCATTACACGCAGAAGGTGCCCTGGAGTTGTTCCTCGGTAACGCCGGAACGGCAATGCGTCCGCTGGCGGCAGCTCTTTGTCTGGGTAGCAATGATATTGTGCTGACCGGTGAGCCGCGCATGAAAGAACGCCCGAtt                                                                                                                       <  2:74331/140‑1 (MQ=255)                  cATTACACGCAGAAGGTGCCCTGGAGTTGTTCCTCGGTAACGCCGGAACGGCAATGCGTCCGCTGGCGGCAGCTCTTTGTCTGGGTAGCAATGATATTGTGCTGACCGGTGAGCCGCGCATGAAAGAACGCCCGATTGGt                                                                                                                    <  1:630669/140‑1 (MQ=255)                   aTTACACGCAGAAGGTGCCCTGGAGTTGTTCCTCGGTAACGCCGGAACGGCAATGCGTCCGCTGGCGGCAGCTCTTTGTCTGGGTAGCAATGATATTGTGCTGACCGGTGAGCCGCGCATGAAAGAACGCCCGATTGGTc                                                                                                                   <  1:702963/140‑1 (MQ=255)                    ttACACGCAGAAGGTGCCCTGGAGTTGTTCCTCGGTAACGCCGGAACGGCAATGCGTCCGCTGGCGGCAGCTCTTTGTCTGGGTAGCAATGATATTGTGCTGACCGGTGAGCCGCGCATGAAAGAACGCCCGATTGGTCa                                                                                                                  <  2:487042/140‑1 (MQ=255)                     tACACGCAGAAGGTGCCCTGGAGTTGTTCCTCGGTAACGCCGGAACGGCAATGCGTCCGCTGGCGGCAGCTCTTTGTCTGGGTAGCAATGATATTGTGCTGACCGGTGAGCCGCGCATGAAAGAACGCCCGATTGGTCAt                                                                                                                 >  1:776278/1‑140 (MQ=255)                          gCAGAAGGTGCCCTGGAGTTGTTCCTCGGTAACGCCGGAACGGCAATGCGTCCGCTGGCGGCAGCTCTTTGTCTGGGTAGCAATGATATTGTGCTGACCGGTGAGCCGCGCATGAAAGAACGCCCGATTGGTCATCtggt                                                                                                            >  1:119231/1‑140 (MQ=255)                            aGAAGGTGCCCTGGAGTTGTTCCTCGGTAACGCCGGAACGGCAATGCGTCCGCTGGCGGCAGCTCTTTGTCTGGGTAGCAATGATATTGTGCTGACCGGTGAGCCGCGCATGAAAGAACGCCCGATTGGTCATCtggtgg                                                                                                          >  1:623491/1‑140 (MQ=255)                             gAAGGTGCCCTGGAGTTGTTCCTCGGTAACGCCGGAACGGCAATGCGTCCGCTGGCGGCAGCTCTTTGTCTGGGTAGCAATGATATTGTGCTGACCGGTGAGCCGCGCATGAAAGAACGCCCGATTGGTCATCTGGTGGa                                                                                                         <  2:30760/140‑1 (MQ=255)                              aaGGTGCCCTGGAGTTGTTCCTCGGTAACGCCGGAACGGCAATGCGTCCGCTGGCGGCAGCTCTTTGTCTGGGTAGCAATGATATTGTGCTGACCGGTGAGCCGCGCATGAAAGAACGCCCGATTGGTCATCTGGTGGAt                                                                                                        >  1:283184/1‑140 (MQ=255)                                 gTGCCCTGGAGTTGTTCCTCGGTAACGCCGGAACGGCAATGCGTCCGCTGGCGGCAGCTCTTTGTCTGGGTAGCAATGATATTGTGCTGACCGGTGAGCCGCGCATGAAAGAACGCCCGATTGGTCATCTGGTGGAtgcg                                                                                                     <  1:911323/140‑1 (MQ=255)                                 gTGCCCTGGAGTTGTTCCTCGGTAACGCCGGAACGGCAATGCGTCCGCTGGCGGCAGCTCTTTGTCTGGGTAGCAATGATATTGTGCTGACCGGTGAGCCGCGCATGAAAGAACGCCCGATTGGTCATCTGGTGGAtgcg                                                                                                     <  2:119231/140‑1 (MQ=255)                                    cccTGGAGTTGTTCCTCGGTAACGCCGGAACGGCAATGCGTCCGCTGGCGGCAGCTCTTTGTCTGGGTAGCAATGATATTGTGCTGACCGGTGAGCCGCGCATGAAAGAACGCCCGATTGGTCATCTGGTGGAtgcgctg                                                                                                  >  1:1119763/1‑140 (MQ=255)                                             tgttCCTCGGTAACGCCGGAACGGCAATGCGTCCGCTGGCGGCAGCTCTTTGTCTGGGTAGCAATGATATTGTGCTGACCGGGGAGCCGCGCATG‑AAGAACGCCCGATTGGTCATCTGGTGGATGCGCTGCGCCTgggcg                                                                                        <  2:27293/140‑1 (MQ=255)                                              gttCCTCGGTAACGCCGGAACGGCAATGCGTCCGCTGGCGGCAGCTCTTTGTCTGGGTAGCAATGATATTGTGCTTACCGGTGAGCCGCGCATGAAAGAACGCCCGATTGGTCATCTGGTGGATGCGCTGCGCCTgggcg                                                                                        <  2:776278/140‑1 (MQ=255)                                              gttCCTCGGTAACGCCGGAACGGCAATGCGTCCGCTGGCGGCAGCTCTTTGTCTGGGTAGCAATGATATTGTGCTGACCGGTGAGCCGCGCATGAAAGAACGCCCGATTGGTCATCTGGTGGAtgcgc                                                                                                    >  1:554858/1‑128 (MQ=255)                                              gttCCTCGGTAACGCCGGAACGGCAATGCGTCCGCTGGCGGCAGCTCTTTGTCTGGGTAGCAATGATATTGTGCTGACCGGTGAGCCGCGCATGAAAGAACGCCCGATTGGTCATCTGGTGGAtgcgc                                                                                                    <  2:554858/128‑1 (MQ=255)                                              gttCCTCGGTAACGCCGGAACGGCAATGCGTCCGCTGGCGGCAGCTCTTTGTCTGGGTAGCAATGATATTGTGCTGACCGGTGAGCCGCGCATGAAAGAACGCCCGATTGGTCATCTGGTGGATGCGCTGCGCCTgggcg                                                                                        >  2:869556/1‑140 (MQ=255)                                              gttCCTCGGTAACGCCGGAACGGCAATGCGTCCGCTGGCGGCAGCTCTTTGTCTGGGTAGCAATGATATTGTGCTGACCGGTGAGCCGCGCATGAAAGAACGCCCGATTGGTCATCTGGTGGATGCGCTGCGCCTgggcg                                                                                        >  2:214492/1‑140 (MQ=255)                                               ttCCTCGGTAACGCCGGAACGGCAATGCGTCCGCTGGCGGCAGCTCTTTGTCTGGGTAGCAATGATATTGTGCTGACCGGTGAGCCGCGCATGAAAGAACGCCCGATTGGTCATCTGGTGGATGCGCTGCGCCTgggcgg                                                                                       >  2:22038/1‑140 (MQ=255)                                                     ggTAACGCCGGAACGGCAATGCGTCCGCTGGCGGCAGCTCTTTGTCTGGGTAGCAATGATATTGTGCTGACCGGTGAGCCGCGCATGAAAGAACGCCCGATTGGTCATCTGGTGGATGCGCTGCGCCTGGGCGGGGCGaa                                                                                 <  1:960517/140‑1 (MQ=255)                                                      gTAACGCCGGAACGGCAATGCGTCCGCTGGCGGCAGCTCTTTGTCTGGGTAGCAATGATATTGTGCTGACCGGTGAGCCGCGCATGAAAGAACGCCCGATTGGTCATCTGGTGGATGCGCTGCGCCTGGGCGGGGCGAAg                                                                                <  2:310995/140‑1 (MQ=255)                                                               gAACGGCAATGCGTCCGCTGGCGGCAGCTCTTTGTCTGGGTAGCAATGATATTGTGCTGACCGGTGAGCCGCGCATGAAAGAACGCCCGATTGGTCATCTGGTGGATGCGCTGCGCCTGGGCGGGGCGAAGATCACTTAc                                                                       <  1:72823/140‑1 (MQ=255)                                                                       aTGCGTCCGCTGGCGGCAGCTCTTTGTCTGGGTAGCAATGATATTGTGCTGACCGGTGAGCCGCGCATGAAAGAACGCCCGATTGGTCATCTGGTGGATGCGCTGCGCCTGGGCGGGGCGAAGATCACTTACCTGGAACa                                                               <  1:547068/140‑1 (MQ=255)                                                                                   gcggcAGCTCTTTGTCTGGGTAGCAATGATATTGTGCTGACCGGTGAGCCGCGCATGAAAGAACGCCCGATTGGTCATCTGGTGGATGCGCTGCGCCTGGGCGGGGCGAAGATCACTTACCTGGAACAAGAAAATTATcc                                                   >  2:111132/1‑140 (MQ=255)                                                                                   gcggcAGCTCTTTGTCTGGGTAGCAATGATATTGTGCTGACCGGTGAGCCGCGCATGAAAGAACGCCCGATTGGTCATCTGGTGGATGCGCTGCGCCTGGGCGGGGCGAAGATCACTTACCTGGAACAAGAAAATTATcc                                                   >  2:1068344/1‑140 (MQ=255)                                                                                   gcggcAGCTCTTTGTCTGGGTAGCAATGATATTGTGCTGACCGGTGAGCCGCGCATGAAAGAACGCCCGATTGGTCATCTGGTGGATGCGCTGCGCCTGGGCGGGGCGAAGATCACTTACCTGGAACAAGAAAATTATcc                                                   >  1:461410/1‑140 (MQ=255)                                                                                      gcAGCTCTTTGTCTGGGTAGCAATGATATTGTGCTGACCGGTGAGCCGCACATGAAAGAACGCCCGATTGGTCATCTGGTGGATGCGCTGCGCCTGGGCGGGCCGAAGAGCTCTTACCTGGAACAAGAAAATTATccgcc                                                <  2:623491/140‑1 (MQ=255)                                                                                        aGCTCTTTGTCTGGGTAGCAATGATATTGTGCTGACCTGTGAGCCGCGCATGAAAGAACGCCCGATTGGTCATCTGGTGGATGCGCTGCGCCTGGGCGGGGCGAAGATCACTTACCTGGAACAAGAAAATTATCCGCCGt                                              <  2:893534/140‑1 (MQ=255)                                                                                        aGCTCTTTGTCTGGGTAGCAATGATATTGTGCTGACCGGTGAGCCGCGCATGAAAGAACGCCCGATTGGTCATCTGGTGGATGCGCTGCGCCTGGGCGGGGCGAAGATCACTTACCTGGAACAAGAAAATTATCCGCCGt                                              <  1:22038/140‑1 (MQ=255)                                                                                                gTCTGGGTAGCAATGATATTGTGCTGACCGGTGAGCCGCGCATGAAAGAACGCCCGATTGGTCATCTGGTGGATGCGCTGCGCCTGGGCGGGGCGAAGATCACTTACCTGGAACAAGAAAATTATCCGCCGTTGCGTTTa                                      <  2:1119763/140‑1 (MQ=255)                                                                                                    gggTAGCAATGATATTGTGCTGACCGGTGAGCCGCGCATGAAAGAACGCCCGATTGGTCATCTGGTGGATGCGCTGCGCCTGGGCGGGGCGAAGATCACTTACCTGGAACAAGAAAATTATCCGCCGTTGCGTTTACAgg                                  >  1:865661/1‑140 (MQ=255)                                                                                                     ggTAGCAATGATATTGTGCTGACCGGTGAGCCGCGCATGAAAGAACGCCCGATTGGTCATCTGGTGGATGCGCTGCGCCTGGGCGGGGCGAAGATCACTTACCTGGAACAAGAAAATTATCCGCCGTTGCGTTTACAggg                                 >  2:996950/1‑140 (MQ=255)                                                                                                           aaTGATATTGTGCTGACCGGTGAGCCGCGCATGAAAGAACGCCCGATTGGTCATCTGGTGGATGCGCTGCGCCTGGGCGGGGCGAAGATCACTTACCTGGAACAAGAAAATTATCCGCCGTTGCGTTTACAGGGCGGCtt                           >  1:436169/1‑140 (MQ=255)                                                                                                                     tgCTGACCGGTGAGCCGCGCATGAAAGAACGCCCGATTGGTCATCTGGTGGATGCGCTGCGCCTGGGCGGGGCGAAGATCACTTACCTGGAACAAGAAAATTATCCGCCGTTGCGTTTACAGGGCGGCTTTACTggcggc                 <  1:214492/140‑1 (MQ=255)                                                                                                                          aCCGGTGAGCCGCGCATGAAAGAACGCCCGATTGGTCATCTGGTGGATGCGCTGCGCCTGGGCGGGGCGAAGATCACTTACCTGGAACAAGAAAATTATCCGCCGTTGCGTTTACAGGGCGGCTTTACTGGCGGCAacgt            >  2:132556/1‑140 (MQ=255)                                                                                                                          aCCGGTGAGCCGCGCATGAAAGAACGCCCGATTGGTCATCTGGTGGATGCGCTGCGCCTGGGCGGGGCGAAGATCACTTACCTGGAACAAGAAAATTATCCGCCGTTGCGTTTACAGGGCGGCTTTACTGGCGGCAacgt            >  1:478373/1‑140 (MQ=255)                                                                                                                          aCCGGTGAGCCGCGCATGAAAGAACGCCCGATTGGTCATCTGGTGGATGCGCTGCGCCTGGGCGGGGCGAAGATCACTTACCTGGAACAAGAAAATTATCCGCCGTTGCGTTTACAGGGCGGCTTTACTGGCGGCAacgt            >  1:1050979/1‑140 (MQ=255)                                                                                                                               tGAGCCGCGCATGAAAGAACGCCCGATTGGTCATCTGGTGGATGCGCTGCGCCTGGGCGGGGCGAAGATCACTTACCTGGAACAAGAAAATTATCCGCCGTTGCGTTTACAGGGCGGCTTTACTGGCGGCAacgttgacg       >  2:330808/1‑140 (MQ=255)                                                                                                                               tGAGCCGCGCATGAAAGAACGCCCGATTGGTCATCTGGTGGATGCGCTGCGCCTGGGCGGGGCGAAGATCACTTACCTGGAACAAGAAAATTATCCGCCGTTGCGTTTACAGGGCGGCTTTACTGGCGGCAacgttg          >  2:365441/1‑137 (MQ=255)                                                                                                                               tGAGCCGCGCATGAAAGAACGCCCGATTGGTCATCTGGTGGATGCGCTGCGCCTGGGCGGGGCGAAGATCACTTACCTGGAACAAGAAAATTATCCGCCGTTGCGTTTACAGGGCGGCTTTACTGGCGGCAacgttg          <  1:365441/137‑1 (MQ=255)                                                                                                                                    cgcgCATGAAAGAACGCCCGATTGGTCATCTGGTGGATGCGCTGCGCCTGGGCGGGGCGAAGATCACTTACCTGGAACAAGAAAATTATCCGCCGTTGCGTTTACAGGGCGGCTTTACTGGCGGCAacgtt           >  2:294723/1‑131 (MQ=255)                                                                                                                                    cgcgCATGAAAGAACGCCCGATTGGTCATCTGGTGGATGCGCTGCGCCTGGGCGGGGCGAAGATCACTTACCTGGAACAAGAAAATTATCCGCCGTTGCGTTTACAGGGCGGCTTTACTGGCGGCAacgtt           <  1:294723/131‑1 (MQ=255)                                                                                                                                    cgcgCATGAAAGAACGCCCGATTGGTCATCTGGTGGATGCGCTGCGCCTGGGCGGGGCGAAGATCACTTACCTGGAACAAGAAAATTATCCGCCGTTGCGTTTACAGGGCGGCTTTACTGGCGGCAACGTTGACGTTGAt  <  1:869556/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        TATCGGTAACGGCGGTCCATTACACGCAGAAGGTGCCCTGGAGTTGTTCCTCGGTAACGCCGGAACGGCAATGCGTCCGCTGGCGGCAGCTCTTTGTCTGGGTAGCAATGATATTGTGCTGACCGGTGAGCCGCGTATGAAAGAACGCCCGATTGGTCATCTGGTGGATGCGCTGCGCCTGGGCGGGGCGAAGATCACTTACCTGGAACAAGAAAATTATCCGCCGTTGCGTTTACAGGGCGGCTTTACTGGCGGCAACGTTGACGTTGAT  >  NC_000913/959036‑959306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑