Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	984,521	A→G	*397Q (TAA→CAA)	aspC ←	aspartate aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	984,521	0	A	G	100.0%	161.7 / NA	43	*397Q (TAA→CAA)	aspC	aspartate aminotransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/22);  total (21/22)

ACAAAATCGTGCAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTACAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATCTGGTGTCATCCCGGCCACATTTACGCGACCAGAAGCAACCGCATATACGCCAAACTCTTCGCGCAGACGCAGCACTTGTTCTTTTGT  >  NC_000913/984393‑984650                                                                                                                                 |                                                                                                                                  aCAAAATCGTGCAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTGCAGCACTGCca                                                                                                                        >  1:732787/1‑140 (MQ=255)   aaaaTCGTGCAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTGCAGCACTGCcaca                                                                                                                      >  1:42157/1‑140 (MQ=255)          tGCAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTGCAGCACTGCCACaa                                                                                                                     >  2:175282/1‑134 (MQ=255)          tGCAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTGCAGCACTGCCACaa                                                                                                                     <  1:175282/134‑1 (MQ=255)            cAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCGGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCg                                                                                                             >  2:699428/1‑140 (MQ=255)            cAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCg                                                                                                             <  1:1031060/140‑1 (MQ=255)              aaTTCAAAATATTGAAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCGca                                                                                                           <  1:226913/140‑1 (MQ=255)               aTTCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCGcac                                                                                                          <  1:974628/140‑1 (MQ=255)                 tCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCGCACAg                                                                                                        <  1:744045/140‑1 (MQ=255)                 tCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCGCACAg                                                                                                        <  1:699428/140‑1 (MQ=255)                   aaaaTATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCACATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCGCACAGCg                                                                                                      <  2:907437/140‑1 (MQ=255)                     aaTATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCGCACAGCGGa                                                                                                    <  2:354264/140‑1 (MQ=255)                      atatTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCGCACAGCGGAg                                                                                                   <  1:30807/140‑1 (MQ=255)                          tGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCGCACAGCGGAGCCAt                                                                                               <  1:542434/140‑1 (MQ=255)                                   cgcgTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATc                                                                                         <  1:404140/137‑1 (MQ=255)                                   cgcgTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATc                                                                                         >  2:404140/1‑137 (MQ=255)                                               aTAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATCTGGTGTCATCCCGGc                                                                          >  1:177648/1‑140 (MQ=255)                                                         cgcATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATCTGGTGTCATCCCGGCCACATTTAcg                                                                >  2:1010571/1‑140 (MQ=255)                                                         cgcATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATCTGGTGTCATCCCGGCCACATTTAcg                                                                >  1:700502/1‑140 (MQ=255)                                                             tCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATCTGGTGTCATCCCGGCCACATTTACGCGAc                                                            >  1:1036059/1‑140 (MQ=255)                                                              cAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATCTGGTGTCATCCCGGCCACATTTACGCGAcc                                                           >  1:905330/1‑140 (MQ=255)                                                                   aaTGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATCTGGTGTCATCCCGGCCACATTTACGCGACCAGAAg                                                      <  2:336884/140‑1 (MQ=255)                                                                    aTGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATCTGGTGTCATCCCGGCCACATTTACGCGACCAGAAGc                                                     <  1:909509/140‑1 (MQ=255)                                                                       ttGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATCTGGTGTCATCCCGGCCACATTTACGCGACCAGAAGCAAc                                                  <  2:732787/140‑1 (MQ=255)                                                                       ttGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATCTGGTGTCATCCCGGCCACATTTACGCGACCAGAAGCAAc                                                  <  1:256071/140‑1 (MQ=255)                                                                               gTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATCTGGTGTCATCCCGGCCACATTTACGCGACCAGAAGCAACCGCAtata                                          >  1:1025131/1‑140 (MQ=255)                                                                                            gCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATCTGGTGTCATCCCGGCCACATTTACGCGACCAGAAGCAACCGCATATACGCCAAACTCTTc                             >  1:840439/1‑140 (MQ=255)                                                                                            gCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATCTGGTGTCATCCCGGCCACATTTACGCGACCAGAAGCAACCGCATATACGCCAAACTCTTc                             >  2:187187/1‑140 (MQ=255)                                                                                                gCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATCTGGTGTCATCCCGGCCACATTTACGCGACCAGAAGCAACCGCATATACGCCAAACTCTTCgcgc                         <  2:730526/140‑1 (MQ=255)                                                                                                 cTTTTCAGCGGGCTTCATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATCTGGTGTCATCCCGGCCACATTTACGCGACCAGAAGCAACCGCATATACGCCAAACTCTTCGCGCa                        >  2:986247/1‑140 (MQ=255)                                                                                                 cTTTTCAGCGGGCTTCATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATCTGGTGTCATCCCGGCCACATTTACGCGACCAGAAGCAACCGCATATACGCCAAACTCTTCGCGCa                        >  2:817849/1‑140 (MQ=255)                                                                                                 cTTTTCAGCGGGCTTCATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATCTGGTGTCATCCCGGCCACATTTACGCGACCAGAAGCAACCGCATATACGCCAAACTCTTCGCGCa                        >  2:648482/1‑140 (MQ=255)                                                                                                 cTTTTCAGCGGGCTTCATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATCTGGTGTCATCCCGGCCACATTTACGCGACCAGAAGCAACCGCATATACGCCAAACTCTTCGCGCa                        >  2:964119/1‑140 (MQ=255)                                                                                                 cTTTTCAGCGGGCTTCATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATCTGGTGTCATCCCGGCCACATTTACGCGACCAGAAGCAACCGCATATACGCCAAACTCTTCGCGCa                        >  1:748792/1‑140 (MQ=255)                                                                                                     tCAGCGGGCTTCATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATCTGGTGTCATCCCGGCCACATTTACGCGACCAGAAGCAACCGCATATACGCCAAACTCTTCGCGCAGACg                    <  2:1025131/140‑1 (MQ=255)                                                                                                        gCGGGCTTCATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATCTGGTGTCATCCCGGCTACATTTACGCGACCAGAAGCAACCGCATATACGCCAAACTCTTCGCGCAGACgcag                 <  2:748792/140‑1 (MQ=255)                                                                                                        gCGGGCTTCATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATCTGGTGTCATCCCGGCCACATTTACGCGACCAGAAGCAACCGCATATACGCCAAACTCTTCGCGCAGACgcag                 <  2:177648/140‑1 (MQ=255)                                                                                                           ggCTTCATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATCTGGTGTCATCCCGGCCACATTTACGCGACCAGAAGCAACCGCATATACGCCAAACTCTTCGCGCAGACGCAGCAc              <  1:846717/140‑1 (MQ=255)                                                                                                            gCTTCATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATCTGGTGTCATCCCGGCCACATTTACGCGACCAGAAGCAACCGCATATACGCCAAACTCTTCGCGCAGACGCAGCACt             >  2:72537/1‑140 (MQ=255)                                                                                                            gCTTCATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATCTGGTGTCATCCCGGCCACATTTACGCGACCAGAAGCAACCGCATATACGCCAAACTCTTCGCGCAGACGCAGCACt             >  1:1017885/1‑140 (MQ=255)                                                                                                              ttCATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATCTGGTGTCATCCCGGCCACATTTACGCGACCAGAAGCAACCGCATATACGCCAAACTCTTCGCGCAGACGCAGCAc              >  2:308290/1‑137 (MQ=255)                                                                                                              ttCATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATCTGGTGTCATCCCGGCCACATTTACGCGACCAGAAGCAACCGCATATACGCCAAACTCTTCGCGCAGACGCAGCAc              <  1:308290/137‑1 (MQ=255)                                                                                                                cATTGTTTTTAATGCTTGCAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATCTGGTGTCATCCCGGCCACATTTACGCGACCAGAAGCAACCGCATATACGCCAAACTCTTCGCGCAGACGCAGCACTTGtt         <  1:1010571/140‑1 (MQ=255)                                                                                                                       tttAATGCTTGCAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATCTGGTGTCATCCCGGCCACATTTACGCGACCAGAAGCAACCGCATATACGCCAAACTCTTCGCGCAGACGCAGCACTTGTTCTTTTGt  <  1:187187/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                  ACAAAATCGTGCAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTACAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATCTGGTGTCATCCCGGCCACATTTACGCGACCAGAAGCAACCGCATATACGCCAAACTCTTCGCGCAGACGCAGCACTTGTTCTTTTGT  >  NC_000913/984393‑984650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑