Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,128,427	G→A	G197S (GGC→AGC)	murJ →	putative lipid II flippase MurJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,128,427	0	G	A	100.0%	73.9 / NA	23	G197S (GGC→AGC)	murJ	putative lipid II flippase MurJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/10);  total (13/10)

TGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGAAACAGATGGGACCGGCGATCCTTGGCGTCTCT  >  NC_000913/1128298‑1128561                                                                                                                                  |                                                                                                                                       tGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCAGCGTCCTGCa                                                                                                                              <  2:429722/140‑1 (MQ=255)     aCCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCAGCGTCCTGCAGCTg                                                                                                                          >  1:686451/1‑140 (MQ=255)         cTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCAGCGTCCTGCAGCTGgtgt                                                                                                                      >  1:573034/1‑140 (MQ=255)           tctcGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCAGCGTCCTGCAGCTGGTGTAt                                                                                                                    >  1:834395/1‑140 (MQ=255)                    cGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCAGCGTCCTGCAGCTGGTGTATCAGCTACCg                                                                                                           <  2:817758/140‑1 (MQ=255)                       cGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCAGCGTCCTGCAGCTGGTGTATCAGCTACCGCAc                                                                                                        <  2:573034/140‑1 (MQ=255)                           tGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCAGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTga                                                                                                    >  1:955493/1‑140 (MQ=255)                           tGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCAGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTga                                                                                                    >  1:174590/1‑140 (MQ=255)                                        cTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCAGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCAt                                                                                       >  1:1021490/1‑140 (MQ=255)                                                         tGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCAGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCa                                                                      <  2:398121/140‑1 (MQ=255)                                                                 cgcTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCAGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGc                                                                            <  2:204364/126‑1 (MQ=255)                                                                 cgcTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCAGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGc                                                                            >  1:204364/1‑126 (MQ=255)                                                                 cgcTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCAGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTc                                                              >  1:636651/1‑140 (MQ=255)                                                                           cGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCAGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCg                                                    >  2:687338/1‑140 (MQ=255)                                                                                 gTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCAGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCaa                                              <  2:432249/140‑1 (MQ=255)                                                                                  tACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCAGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAAt                                             >  1:659428/1‑140 (MQ=255)                                                                                       tAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCAGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGcgcg                                        <  2:126681/140‑1 (MQ=255)                                                                                       tAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCAGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGcgcg                                        <  2:659428/140‑1 (MQ=255)                                                                                             aCCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCAGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGa                                  >  1:751975/1‑140 (MQ=255)                                                                                             aCCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCAGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGa                                  >  1:247972/1‑140 (MQ=255)                                                                                                    ctggcgctggcgTGGGCTGTTACGGTCGGCAGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGAAACAGAt                           >  1:939884/1‑140 (MQ=255)                                                                                                                        tACGGTCGGCAGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGAAACAGATGGGACCGGCGATCCTTGGCg       <  2:247972/140‑1 (MQ=255)                                                                                                                             tCGGCAGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGAAACAGATGGGACCGGCGAACCTTGGCGTctct  <  2:686451/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                       TGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGAAACAGATGGGACCGGCGATCCTTGGCGTCTCT  >  NC_000913/1128298‑1128561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑