Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,302,122	G→A	R314H (CGT→CAT)	oppA →	oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,302,122	0	G	A	100.0%	255.9 / NA	74	R314H (CGT→CAT)	oppA	oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/40);  total (34/40)

AATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAG  >  NC_000913/1301992‑1302246                                                                                                                                   |                                                                                                                             aaaaaCGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTAc                                                                                                                     <  1:271139/137‑1 (MQ=255) aaTAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTAc                                                                                                                     <  2:749719/140‑1 (MQ=255) aaTAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTAc                                                                                                                     <  1:181351/140‑1 (MQ=255) aaTAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTAc                                                                                                                     <  2:667881/140‑1 (MQ=255)         cATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGa                                                                                                             >  2:687487/1‑140 (MQ=255)         cATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGa                                                                                                             >  1:388879/1‑140 (MQ=255)           tGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGaaa                                                                                                           >  2:251262/1‑140 (MQ=255)           tGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGaaa                                                                                                           >  2:310157/1‑140 (MQ=255)           tGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGaaa                                                                                                           >  1:865962/1‑140 (MQ=255)            gCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtgtgcatgtgcg                                                                                                                        <  2:233937/126‑1 (MQ=255)            gCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtgtgcatgtgcg                                                                                                                        >  1:233937/1‑126 (MQ=255)            gCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAAc                                                                                                          <  2:865962/140‑1 (MQ=255)              cGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTa                                                                                                        >  2:193238/1‑140 (MQ=255)                aTCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAgg                                                                                                      >  1:398213/1‑140 (MQ=255)                aTCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAgg                                                                                                      >  2:641282/1‑140 (MQ=255)                   aaaTTTTTCCA‑AAACCGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATg                                                                                                  <  1:148190/139‑1 (MQ=255)                       tGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGAc                                                                                               <  2:57281/140‑1 (MQ=255)                       tGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGAc                                                                                               <  2:50925/140‑1 (MQ=255)                         ttCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACcg                                                                                             <  1:19877/140‑1 (MQ=255)                              gAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGata                                                                                        <  1:792640/140‑1 (MQ=255)                               aaGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACcg                                                                                             <  2:1123508/134‑1 (MQ=255)                               aaGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACcg                                                                                             >  1:1123508/1‑134 (MQ=255)                               aaGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGatat                                                                                       >  2:1067277/1‑140 (MQ=255)                                aGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCTCTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATc                                                                                      <  2:388879/140‑1 (MQ=255)                                aGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATc                                                                                      <  1:643821/140‑1 (MQ=255)                                aGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATc                                                                                      <  2:441554/140‑1 (MQ=255)                                    gAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTg                                                                                  <  1:497153/140‑1 (MQ=255)                                    gAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTg                                                                                  >  1:519538/1‑140 (MQ=255)                                        aaaGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTtaa                                                                              <  1:120340/140‑1 (MQ=255)                                         aaGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTtaat                                                                             <  2:398213/140‑1 (MQ=255)                                                 ccGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGaa                                                                     >  2:1055953/1‑140 (MQ=255)                                                         aGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTtaataa                                                                           <  1:210156/126‑1 (MQ=255)                                                         aGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTtaataa                                                                           >  2:210156/1‑126 (MQ=255)                                                                   gATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGcc                                                   >  2:230599/1‑140 (MQ=255)                                                                   gATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGcc                                                   <  1:310157/140‑1 (MQ=255)                                                                      ccATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGc                                                >  1:1068103/1‑140 (MQ=255)                                                                       cATACCTGTGCACTTACTATTCCAAAATAAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGcc                                               <  2:394159/140‑1 (MQ=255)                                                                       cATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGcc                                               <  1:1055953/140‑1 (MQ=255)                                                                          aCCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAAc                                                        <  2:218186/128‑1 (MQ=255)                                                                          aCCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAAc                                                        >  1:218186/1‑128 (MQ=255)                                                                          aCCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTAt                                            >  1:394159/1‑140 (MQ=255)                                                                            cTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATgg                                          >  1:388863/1‑140 (MQ=255)                                                                            cTGTGCACTTACTATTACGAAATTAACAACCAAAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATgg                                          <  1:1030590/140‑1 (MQ=255)                                                                             tgtgCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGt                                         <  1:35007/140‑1 (MQ=255)                                                                              gtgCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGcc                                                   <  1:205855/129‑1 (MQ=255)                                                                              gtgCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGcc                                                   >  2:205855/1‑129 (MQ=255)                                                                              gtgCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGtt                                        >  1:164675/1‑140 (MQ=255)                                                                              gtgCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGtt                                        >  2:503280/1‑140 (MQ=255)                                                                              gtgCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGtt                                        >  2:783850/1‑140 (MQ=255)                                                                               tgCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCg                                                 >  2:109963/1‑130 (MQ=255)                                                                               tgCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCg                                                 <  1:109963/130‑1 (MQ=255)                                                                                 cACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTaca                                     <  1:193238/140‑1 (MQ=255)                                                                                 cACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTaca                                     >  2:721995/1‑140 (MQ=255)                                                                                  aCTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGtt                                        >  2:732851/1‑136 (MQ=255)                                                                                  aCTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGtt                                        <  1:732851/136‑1 (MQ=255)                                                                                   cTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACt                                   >  1:903211/1‑140 (MQ=255)                                                                                   cTTACTATTACAAAATAAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACt                                   <  1:244965/140‑1 (MQ=255)                                                                                    ttACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACt                                   <  1:844538/139‑1 (MQ=255)                                                                                    ttACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACt                                   >  2:844538/1‑139 (MQ=255)                                                                                      tgtATTAAGAATTTAACCCCCAGAACCCCCCATTTACCGTTGTGCTTGTGGGTCCCGCTCTGCACCTAGGTTTGGCCCGGGTTTTAATTGTTAATAAAGTGAAAGGGCGGGGGAACGTGCCCGCCTATGGTTACACTCCa                                <  2:903210/138‑1 (MQ=255)                                                                                      attATTACAAAATTAACAACCAAAACCCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCa                                <  2:1088206/138‑1 (MQ=255)                                                                                      aCTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATgg                                          <  1:870223/130‑1 (MQ=255)                                                                                      aCTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATgg                                          >  2:870223/1‑130 (MQ=255)                                                                                      aCTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCa                                <  1:92358/140‑1 (MQ=255)                                                                                      aCTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCa                                <  1:641282/140‑1 (MQ=255)                                                                                      aCTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCa                                >  1:314912/1‑140 (MQ=255)                                                                                      aCTATTACAAAATAAACAACCAAAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCa                                <  2:903211/140‑1 (MQ=255)                                                                                              gAAATTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATAc                        >  1:1088206/1‑140 (MQ=255)                                                                                                 aTTAACAACCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGa                     >  2:968080/1‑140 (MQ=255)                                                                                                     acaacCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGgc                 >  2:872198/1‑140 (MQ=255)                                                                                                      cacccAAAACCCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGgcg                <  2:388863/137‑1 (MQ=255)                                                                                                      caacCAGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGgcg                <  1:1067277/140‑1 (MQ=255)                                                                                                           aGAACCCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCaaaa           <  2:84583/140‑1 (MQ=255)                                                                                                           aGAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCaaaa           <  1:230599/140‑1 (MQ=255)                                                                                                            gAAACCGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAAt          >  1:491531/1‑140 (MQ=255)                                                                                                                ccGCCATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGAc      <  1:503510/140‑1 (MQ=255)                                                                                                                    cATTCAACGATGTGCATGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAg  >  2:218433/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                             AATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAG  >  NC_000913/1301992‑1302246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑