Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,755,515	G→A	intergenic (‑374/‑183)	fumD ← / → pykF	fumarase D/pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,755,515	0	G	A	100.0%	183.7 / NA	52	intergenic (‑374/‑183)	fumD/pykF	fumarase D/pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/29);  total (23/29)

TGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCA  >  NC_000913/1755392‑1755641                                                                                                                            |                                                                                                                               tGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCAc                                                                                                                <  1:213846/140‑1 (MQ=255)    cTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCTATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCa                                                                                                             >  2:404292/1‑140 (MQ=255)      aaCAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCCCTCACg                                                                                                           <  2:173295/140‑1 (MQ=255)       aCAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGt                                                                                                          >  2:754269/1‑140 (MQ=255)            ttGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGc                                                                                                     <  1:654955/140‑1 (MQ=255)            ttGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGc                                                                                                     <  2:1130189/140‑1 (MQ=255)             tGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACt                                                                                                               >  1:19898/1‑129 (MQ=255)             tGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACt                                                                                                               <  2:19898/129‑1 (MQ=255)             tGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCt                                                                                                    >  1:123183/1‑140 (MQ=255)             tGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCt                                                                                                    >  1:366679/1‑140 (MQ=255)               tataTTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAgg                                                                                                                   >  2:474249/1‑123 (MQ=255)               tataTTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAgg                                                                                                                   <  1:474249/123‑1 (MQ=255)                 tatTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCTgaga                                                                                                <  2:377308/140‑1 (MQ=255)                 tatTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCTgaga                                                                                                >  1:542674/1‑140 (MQ=255)                   ttttttGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCTATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCTGAGaca                                                                                              <  1:404292/140‑1 (MQ=255)                      tttGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCTGAGACACaa                                                                                           <  1:623637/140‑1 (MQ=255)                         gAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGtt                                                                                                         >  1:1131457/1‑123 (MQ=255)                         gAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGtt                                                                                                         <  2:1131457/123‑1 (MQ=255)                                     ttgttttCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTg                                                                            <  2:1078764/140‑1 (MQ=255)                                       gttttCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCa                                                                          >  2:1000429/1‑140 (MQ=255)                                        ttttCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCTGAGACACaa                                                                                           >  2:199362/1‑122 (MQ=255)                                        ttttCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCTGAGACACaa                                                                                           <  1:199362/122‑1 (MQ=255)                                        ttttCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCAc                                                                         <  2:123183/140‑1 (MQ=255)                                         tttCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACg                                                                        <  2:123287/140‑1 (MQ=255)                                            ccTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCtt                                                                     >  1:954323/1‑140 (MQ=255)                                                  ggATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGa                                                               >  2:822254/1‑140 (MQ=255)                                                     ttAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGt                                                            <  2:205888/140‑1 (MQ=255)                                                     ttAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGt                                                            <  1:754269/140‑1 (MQ=255)                                                       aaTTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCa                                                          <  1:896373/140‑1 (MQ=255)                                                          ttCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCt                                                       <  1:822254/140‑1 (MQ=255)                                                              gCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATcc                                                   >  1:1136157/1‑140 (MQ=255)                                                                  ataATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCAcc                                                        >  2:983951/1‑131 (MQ=255)                                                                  ataATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCAcc                                                        <  1:983951/131‑1 (MQ=255)                                                                    aaTGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTagag                                             >  1:341826/1‑140 (MQ=255)                                                                         gcgcCAATTGACTCTTTAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAgg                                        >  1:116703/1‑140 (MQ=255)                                                                         gcgcCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTagag                                             >  1:690357/1‑135 (MQ=255)                                                                         gcgcCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTagag                                             <  2:690357/135‑1 (MQ=255)                                                                         gcgcCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAgg                                        >  1:29087/1‑140 (MQ=255)                                                                         gcgcCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAgg                                        <  2:575453/140‑1 (MQ=255)                                                                           gcCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCt                                                       <  1:484887/123‑1 (MQ=255)                                                                           gcCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCt                                                       >  2:484887/1‑123 (MQ=255)                                                                           gcCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTa                                                >  1:260791/1‑130 (MQ=255)                                                                           gcCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTa                                                <  2:260791/130‑1 (MQ=255)                                                                            ccAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGcac                                     >  1:694829/1‑140 (MQ=255)                                                                             cAATTGACTCTTTAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGcacc                                    <  2:116703/140‑1 (MQ=255)                                                                              aaTTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGcacca                                   <  1:1000429/140‑1 (MQ=255)                                                                               aTTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGcaccac                                  <  2:1136157/140‑1 (MQ=255)                                                                                    ctctTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCTGCGACACAAGCACACATGCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACtt                             >  2:982485/1‑140 (MQ=255)                                                                                       ttGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCg                          >  1:753193/1‑140 (MQ=255)                                                                                        tGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGt                         <  1:988517/140‑1 (MQ=255)                                                                                                     cACTTTGGACTGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCg            <  2:341826/140‑1 (MQ=255)                                                                                                               tGTAGAACTCAACAACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCa  <  2:954323/140‑1 (MQ=255)                                                                                                                            |                                                                                                                               TGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCA  >  NC_000913/1755392‑1755641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑