Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,761,634	G→A	P53L (CCG→CTG)	sufD ←	Fe‑S cluster scaffold complex subunit SufD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,761,634	0	G	A	100.0%	161.8 / NA	46	P53L (CCG→CTG)	sufD	Fe‑S cluster scaffold complex subunit SufD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/17);  total (29/17)

CGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCAAACAAGTGATGCCACTGTTGCAGCGCGTTA  >  NC_000913/1761497‑1761768                                                                                                                                          |                                                                                                                                       cGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCagcagc                                                                                                                                      >  1:339404/1‑140 (MQ=255)  gCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAgcagcg                                                                                                                                     <  2:1055070/140‑1 (MQ=255)        tGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATAtt                                                                                                                               <  1:761439/140‑1 (MQ=255)          cGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTc                                                                                                                             >  1:823511/1‑140 (MQ=255)                cGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGttt                                                                                                                       <  1:955275/140‑1 (MQ=255)                  tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAgcagcg                                                                                                                                     >  2:660777/1‑124 (MQ=255)                  tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAgcagcg                                                                                                                                     <  1:660777/124‑1 (MQ=255)                  tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTc                                                                                                                     >  2:614828/1‑140 (MQ=255)                  tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTc                                                                                                                     >  1:588227/1‑140 (MQ=255)                  tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGGGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTc                                                                                                                     >  2:998607/1‑140 (MQ=255)                       cccATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGtt                                                                                                                <  1:278973/140‑1 (MQ=255)                         cATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTa                                                                                                              <  2:453646/140‑1 (MQ=255)                           tCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACg                                                                                                            >  1:703901/1‑140 (MQ=255)                           tCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACg                                                                                                            >  1:445875/1‑140 (MQ=255)                                 aaCACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCgg                                                                                                      <  2:588227/140‑1 (MQ=255)                                   cacCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCa                                                                                                    <  2:1091989/140‑1 (MQ=255)                                      cAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTc                                                                                                 >  2:984345/1‑140 (MQ=255)                                      cAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGCGATATCTCTCCCGCAATGCTGACAAACTCGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTc                                                                                                 >  2:457281/1‑140 (MQ=255)                                          cGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGt                                                                                             <  1:324229/140‑1 (MQ=255)                                            cACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTAc                                                                                           >  2:11173/1‑140 (MQ=255)                                                           tAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTAc                                                                                           <  2:482375/125‑1 (MQ=255)                                                           tAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTAc                                                                                           >  1:482375/1‑125 (MQ=255)                                                              cGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAAt                                                                         <  2:703901/140‑1 (MQ=255)                                                                  aaGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAAtgctg                                                                     >  2:749310/1‑140 (MQ=255)                                                                   aGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAAtgctgc                                                                    <  2:396863/140‑1 (MQ=255)                                                                               gctgTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCggg                                                        >  1:756931/1‑140 (MQ=255)                                                                                          atCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGt                                             >  2:835939/1‑140 (MQ=255)                                                                                          atCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGt                                             >  1:822141/1‑140 (MQ=255)                                                                                                 ccGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCa                                      >  2:297329/1‑140 (MQ=255)                                                                                                 ccGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCa                                      >  1:6471/1‑140 (MQ=255)                                                                                                    cAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCt                                   <  2:822141/140‑1 (MQ=255)                                                                                                      aTGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTc                                 >  2:561114/1‑140 (MQ=255)                                                                                                       tGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCa                                >  1:118824/1‑140 (MQ=255)                                                                                                       tGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCa                                <  1:749310/140‑1 (MQ=255)                                                                                                         cTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCaaa                              >  1:449195/1‑140 (MQ=255)                                                                                                                 cTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCAAACAAGTGAt                      >  2:595312/1‑140 (MQ=255)                                                                                                                 cTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCAAACAAGTGAt                      <  1:297329/140‑1 (MQ=255)                                                                                                                 cTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCAAACAAGTGAt                      >  2:680451/1‑140 (MQ=255)                                                                                                                 cTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCAAACAAGTGAt                      >  2:52400/1‑140 (MQ=255)                                                                                                                 cTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCAAACAAGTGAt                      >  1:873812/1‑140 (MQ=255)                                                                                                                   ggCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCAAACAAGTGATGc                    >  2:629871/1‑140 (MQ=255)                                                                                                                   ggCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCAAACAAGTGATGc                    <  1:998607/140‑1 (MQ=255)                                                                                                                              aGCCTTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCAAACAAGTGATGCCACTGTTGCAg         >  2:42342/1‑140 (MQ=255)                                                                                                                              aGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCAAACAAGTGATGCCACTGTTGCAg         >  1:602676/1‑140 (MQ=255)                                                                                                                              aGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCAAACAAGTGATGCCACTGTTGCAg         >  2:757529/1‑140 (MQ=255)                                                                                                                                cccTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCAAACAAGTGATGCCACTGTTGCAgcg       >  2:561662/1‑140 (MQ=255)                                                                                                                                cccTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCAAACAAGTGATGCCACTGTTGCAgcg       >  2:545721/1‑140 (MQ=255)                                                                                                                                cccTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCAAACAAGTGATGCCACTGTTGCAgcg       <  2:873812/140‑1 (MQ=255)                                                                                                                                   ttCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCAAACAAGTGATGCCACTGTTGCAGCGCGt    <  1:757529/140‑1 (MQ=255)                                                                                                                                   ttCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCAAACAAGTGATGCCACTGTTGCAGCGCGt    <  2:602676/140‑1 (MQ=255)                                                                                                                                     ccAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCAAACAAGTGATGCCACTGTTGCAGCGCGTTa  >  2:1058888/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       CGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCAAACAAGTGATGCCACTGTTGCAGCGCGTTA  >  NC_000913/1761497‑1761768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑