Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,771,496	A→G	G142G (GGA→GGG)	ydiM →	putative exporter YdiM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,771,496	0	A	G	100.0%	217.2 / NA	58	G142G (GGA→GGG)	ydiM	putative exporter YdiM
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/30);  total (28/30)

ATCGCTTATGTTTTTGGCTTTCTGGCGGGAATGGCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGGGCTGAACTGTGGTTCGGTTGGTCCTTTATGATTGCTGCAGGCATTATGTTTATTAACGCTCTGTTTTTATACCGTTGTACGTTC  >  NC_000913/1771359‑1771619                                                                                                                                          |                                                                                                                            aTCGCTTATGTTTTTGGCTTTCTGGCGGGAATGGCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCa                                                                                                                           >  1:721271/1‑140 (MQ=255)     cTTATGTTTTTGGCTTTCTGGCGGGAATGGCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAAttt                                                                                                                       >  2:329648/1‑140 (MQ=255)       tATGTTTTTGGCTTTCTGGCGGGAATGGCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAAttttt                                                                                                                     >  1:597614/1‑140 (MQ=255)        aTGTTTTTGGCTTTCTGGCGGGAATGGCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTtta                                                                                                                    >  2:73719/1‑140 (MQ=255)        aTGTTTTTGGCTTTCTGGCGGGAATGGCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTtta                                                                                                                    >  1:809365/1‑140 (MQ=255)        aTGTTTTTGGCTTTCTGGCGGGAATGGCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTtta                                                                                                                    >  2:357781/1‑140 (MQ=255)         tGTTTTTGGCTTTCTGGCGGGAATGGCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTttat                                                                                                                   <  1:357781/140‑1 (MQ=255)          gTTTTTGGCTTTCTGGCGGGAATGGCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTttatt                                                                                                                  <  2:321589/140‑1 (MQ=255)            ttttGGCTTTCTGGCGGGAATGGCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTAc                                                                                                                <  1:112651/140‑1 (MQ=255)              ttGGCTTTCTGGCGGGAATGGCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCg                                                                                                              <  2:1069892/140‑1 (MQ=255)              ttGGCTTTCTGGCGGGAATGGCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCg                                                                                                              >  1:876426/1‑140 (MQ=255)              ttGGCTTTCTGGCGGGAATGGCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCg                                                                                                              <  2:676702/140‑1 (MQ=255)               tGGCTTTCTGGCGGGAATGGCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGc                                                                                                             <  1:1121608/140‑1 (MQ=255)                ggCTTTCTGGCGGGAATGGCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTttat                                                                                                                   <  1:423983/133‑1 (MQ=255)                ggCTTTCTGGCGGGAATGGCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTttat                                                                                                                   >  2:423983/1‑133 (MQ=255)                ggCTTTCTGGCGGGAATGGCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCt                                                                                                            <  1:1015403/140‑1 (MQ=255)                      cTGGCGGGAATGGCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCAt                                                                                                      <  2:389077/140‑1 (MQ=255)                        ggCGGGAATGGCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTa                                                                                                    <  1:73719/140‑1 (MQ=255)                            ggAATGGCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCt                                                                                                >  1:922850/1‑140 (MQ=255)                                tGGCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTa                                                                                            >  1:350650/1‑140 (MQ=255)                                  gCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAgt                                                                                          <  2:350650/140‑1 (MQ=255)                                            ttCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGGGCTGaa                                                                                <  2:174915/140‑1 (MQ=255)                                            ttCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGGGCTGaa                                                                                >  1:932950/1‑140 (MQ=255)                                                  aTGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGAGCTGAACTGTgg                                                                          >  2:641749/1‑140 (MQ=255)                                                    gCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTggg                                                                                     <  1:1063417/127‑1 (MQ=255)                                                    gCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTggg                                                                                     >  2:1063417/1‑127 (MQ=255)                                                    gCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGGGCTGAACTGTGGtt                                                                        >  2:1110199/1‑140 (MQ=255)                                                          aCTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGGGCTGAACTGTGGTTCGGTTg                                                                  <  1:140354/140‑1 (MQ=255)                                                           cTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGGGCTGAACTGTGGTTCGGTTgg                                                                 <  1:1110199/140‑1 (MQ=255)                                                            ttATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGGGCTGAACTGTGGTTCGGTTGGt                                                                <  1:645536/140‑1 (MQ=255)                                                             tATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGGGCTGAACTGTGGt                                                                         >  1:191292/1‑130 (MQ=255)                                                             tATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGGGCTGAACTGTGGt                                                                         <  2:191292/130‑1 (MQ=255)                                                                cccAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGGGCTGAACTGTGGTTCGGTTGGTCCtt                                                            <  2:818033/140‑1 (MQ=255)                                                                     tttGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGGGCTGAACTGTGGTTCGGTTGGTCCTTTATGa                                                       >  2:1104936/1‑140 (MQ=255)                                                                      ttGATGGAAGCTTTTCCACCCTCACCCGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGGGCTGAACTGTGGTTCGGTTGGTCCTTTATGAt                                                      <  2:53177/140‑1 (MQ=255)                                                                         aTGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGGGCTGAACTGTGGTTCGGTTGGTCCTTTATGATtgc                                                   <  1:329648/140‑1 (MQ=255)                                                                          tGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGGGCTGAACTGTGGTTCGGTTGGTCCTTTATGATtgct                                                  >  2:291243/1‑140 (MQ=255)                                                                           ggAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGGGCTGAACTGTGGTTCGGTTGGTCCTTTATGATtgctg                                                 <  1:939801/140‑1 (MQ=255)                                                                               gCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGGGCTGAACTGTGGTTCGGTTGGTCCTTTATGATTGCTGCAgg                                             >  2:85389/1‑140 (MQ=255)                                                                                         gCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGGGCTGAACTGTGGTTCGGTTGGTCCTTTATGATTGCTGCAGGCATTATGttt                                   >  1:958635/1‑140 (MQ=255)                                                                                           tCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCTAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGGGCTGAACTGTGGTTCGGTTGGTCCTTTATGATTGCTGCAGGCATTATGTttat                                 <  1:12347/140‑1 (MQ=255)                                                                                             aCCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGGGCTGAACTGTGGTTCGGTTGGTCCTTTATGATTGCTGCAGGCATTATGTttatta                               >  2:175068/1‑140 (MQ=255)                                                                                             aCCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGGGCTGAACTGTGGTTCGGTTGGTCCTTTATGATTGCTGCAGGCATTATGTttatta                               >  1:903282/1‑140 (MQ=255)                                                                                                  ggACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGGGCTGAACTGTGGTTCGGTTGGTCCTTTATGATTGCTGCAGGCATTATGTTTATTAACGct                          >  1:794605/1‑140 (MQ=255)                                                                                                   gACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGGGCTGAACTGTGGTTCGGTTGGTCCTTTATGATTGCTGCAGGCATTATGTTTATTAACGctc                         <  1:244299/140‑1 (MQ=255)                                                                                                     cAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGGGCTGAACTGTGGTTCGGTTGGTCCTTTATGATTGCTGCAGGCATTATGTTTATTAACGCTCTg                       >  2:1133494/1‑140 (MQ=255)                                                                                                      aGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGGGCTGAACTGTGGTTCGGTTGGTCCTTTATGATTGCTGCAGGCATTATGTTTATTAACGCTCTGt                      <  1:867282/140‑1 (MQ=255)                                                                                                       gCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGGGCTGAACTGTGGTTCGGTTGGTCCTTTATGATTGCTGCAGGCATTATGTttatta                               >  1:473002/1‑130 (MQ=255)                                                                                                       gCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGGGCTGAACTGTGGTTCGGTTGGTCCTTTATGATTGCTGCAGGCATTATGTttatta                               <  2:473002/130‑1 (MQ=255)                                                                                                       gCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGGGCTGAACTGTGGTTCGGTTGGTCCTTTATGATTGCTGCAGGCATTATGTTTATTAACGCTCTGt                      <  1:980386/139‑1 (MQ=255)                                                                                                       gCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGGGCTGAACTGTGGTTCGGTTGGTCCTTTATGATTGCTGCAGGCATTATGTTTATTAACGCTCTGt                      >  2:980386/1‑139 (MQ=255)                                                                                                         cAATATTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGGGCTGAACTGTGGTTCGGTTGGTCCTTTATGATTGCTGCAGGCATTATGTTTATTAACGCTCTGtttt                   >  1:958103/1‑140 (MQ=255)                                                                                                           atatTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGGGCTGAACTGTGGTTCGGTTGGTCCTTTATGATTGCTGCAGGCATTATGTTTATTAACGCTCTGTTTTta                 <  2:992583/140‑1 (MQ=255)                                                                                                             atTTTAATTAAAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGGGCTGAACTGTGGTTCGGTTGGTCCTTTATGATTGCTGCAGGCATTATGTTTATTAACGCTCTGTTTTtata               <  2:922850/140‑1 (MQ=255)                                                                                                                ttaattaaAGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGGGCTGAACTGTGGTTCGGTTGGTCCTTTATGATTGCTGCAGGCATTATGTTTATTAACGCTCTGTTTTTATACCg            <  1:1104936/140‑1 (MQ=255)                                                                                                                        aGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGGGCTGAACTGTGGTTCGGTTGGTCCTTTATGATTGCTGCAGGCATTATGTTTATTAACGCTCTGTTTTTATACCGTTg         <  2:494379/135‑1 (MQ=255)                                                                                                                        aGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGGGCTGAACTGTGGTTCGGTTGGTCCTTTATGATTGCTGCAGGCATTATGTTTATTAACGCTCTGTTTTTATACCGTTg         >  1:494379/1‑135 (MQ=255)                                                                                                                        aGCATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGGGCTGAACTGTGGTTCGGTTGGTCCTTTATGATTGCTGCAGGCATTATGTTTATTAACGCTCTGTTTTTATACCGTTGTACGt    >  2:848135/1‑140 (MQ=255)                                                                                                                          cATTTGTTTCCAGCGGGCAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGGGCTGAACTGTGGTTCGGTTGGTCCTTTATGATTGCTGCAGGCATTATGTTTATTAACGCTCTGTTTTTATACCGTTGTACGTTc  <  1:291243/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                            ATCGCTTATGTTTTTGGCTTTCTGGCGGGAATGGCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGGGCTGAACTGTGGTTCGGTTGGTCCTTTATGATTGCTGCAGGCATTATGTTTATTAACGCTCTGTTTTTATACCGTTGTACGTTC  >  NC_000913/1771359‑1771619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑