Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,820,296	T→C	S307G (AGT→GGT)	chbC ←	N,N'‑diacetylchitobiose‑specific PTS enzyme IIC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,820,296	0	T	C	100.0%	243.4 / NA	65	S307G (AGT→GGT)	chbC	N,N'‑diacetylchitobiose‑specific PTS enzyme IIC component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/36);  total (29/36)

ATTGGCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACTGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGCGCCGCTTCGACGGAACCATATTGCTGATAGGTCGCGATATTTTCCAGTGCCCACGGCGTCATAATG  >  NC_000913/1820163‑1820432                                                                                                                                      |                                                                                                                                         aTTGGCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGccccc                                                                                                                                    <  2:759839/140‑1 (MQ=255)  ttGGCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCa                                                                                                                                   >  1:215039/1‑140 (MQ=255)   tGGCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCGCCACCGCCCCCaa                                                                                                                                  <  2:858922/140‑1 (MQ=255)    ggCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAg                                                                                                                                 <  1:1061330/140‑1 (MQ=255)    ggCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAg                                                                                                                                 <  2:411513/140‑1 (MQ=255)      cAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCGGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGa                                                                                                                               <  2:467887/140‑1 (MQ=255)       aGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGaa                                                                                                                              >  2:972831/1‑140 (MQ=255)         aCCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGaaaa                                                                                                                            <  2:234256/140‑1 (MQ=255)         aCCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGaaaa                                                                                                                            >  1:422326/1‑140 (MQ=255)              aCAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAg                                                                                                                       <  1:591295/140‑1 (MQ=255)                       gTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGTAAAATaa                                                                                                                         <  1:71404/129‑1 (MQ=255)                       gTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGTAAAATaa                                                                                                                         >  2:71404/1‑129 (MQ=255)                              aaTCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCt                                                                                                       <  1:158815/140‑1 (MQ=255)                              aaTCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCt                                                                                                       <  2:924155/140‑1 (MQ=255)                                   ggAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGcc                                                                                                  <  2:676170/140‑1 (MQ=255)                                    gAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGACCAGGCCTAAAGTCGCACCCCCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGccc                                                                                                 <  2:1012873/140‑1 (MQ=255)                                         tGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCt                                                                                                       <  2:323202/129‑1 (MQ=255)                                         tGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCt                                                                                                       >  1:323202/1‑129 (MQ=255)                                         tGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGccc                                                                                                 <  1:171123/135‑1 (MQ=255)                                         tGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGccc                                                                                                 >  2:171123/1‑135 (MQ=255)                                         tGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGcc                                                                                                  >  2:550842/1‑134 (MQ=255)                                         tGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGcc                                                                                                  <  1:550842/134‑1 (MQ=255)                                         tGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGata                                                                                            >  1:853518/1‑140 (MQ=255)                                            cGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATgg                                                                                         <  1:1003832/140‑1 (MQ=255)                                            cGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATgg                                                                                         >  2:716921/1‑140 (MQ=255)                                            cGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAATCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATgg                                                                                         >  2:916192/1‑140 (MQ=255)                                               aCGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAg                                                                                      <  2:160868/140‑1 (MQ=255)                                                   cAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGa                                                                                        <  1:723449/134‑1 (MQ=255)                                                   cAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGa                                                                                        >  2:723449/1‑134 (MQ=255)                                                           gTTTTGCCACCTGACGATAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCt                                                                          >  2:1048721/1‑140 (MQ=255)                                                           gTTTTGCCACCTGACGATAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAAGTCTTACCGGCt                                                                          >  2:544075/1‑140 (MQ=255)                                                            ttttGCCACCTGACGATAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTg                                                                         >  2:216543/1‑140 (MQ=255)                                                                gCCACCTGACGATAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAg                                                                     <  1:424447/140‑1 (MQ=255)                                                                  cACCTGACGATAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAgcg                                                                   <  2:1135170/140‑1 (MQ=255)                                                                        aCGATAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGCGCCGCtt                                                             >  2:907311/1‑140 (MQ=255)                                                                           aTAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGCGCCGCTTCGa                                                          <  1:1048721/140‑1 (MQ=255)                                                                           aTAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGCGCCGCTTCGa                                                          <  1:114327/140‑1 (MQ=255)                                                                             aaTCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGCGCCGCTTCGACg                                                        <  2:853518/140‑1 (MQ=255)                                                                              aTCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGCGCCGCTTCGACgg                                                       <  2:215039/140‑1 (MQ=255)                                                                              aTCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCACAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGCGCCGCTTCGACgg                                                       >  2:1015072/1‑140 (MQ=255)                                                                                cAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGCGCCGCTTCGACGGaa                                                     <  1:216543/140‑1 (MQ=255)                                                                                  gCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAACTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGCGCCGCTTCGACGGAAcc                                                   <  2:1047468/140‑1 (MQ=255)                                                                                   cACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGCGCCGCTTCGACGGa                                                      >  2:470713/1‑136 (MQ=255)                                                                                   cACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGCGCCGCTTCGACGGa                                                      <  1:470713/136‑1 (MQ=255)                                                                                      ggCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGCGCCGCTTCGACGGAACCatat                                               >  2:773674/1‑140 (MQ=255)                                                                                        cGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGCGCCGCTTCGACGGAACCATATTg                                             >  1:1135804/1‑140 (MQ=255)                                                                                           gagGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGCGCCGCTTCGACGGaa                                                     <  1:620986/129‑1 (MQ=255)                                                                                           gagGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGCGCCGCTTCGACGGaa                                                     >  2:620986/1‑129 (MQ=255)                                                                                             ggCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGCGCCGCTTCGACGGAACCATATTGCTGAt                                        <  1:1015072/140‑1 (MQ=255)                                                                                                   aaaGATAGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGCGCCGCTTCGACGGAACCATATTGCTGATAGGTcg                                  <  2:1135804/140‑1 (MQ=255)                                                                                                         aGCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAAGTCTTACCGGCTGCCAGCGCCGCTTCGACGGAACCATATTGCTGATAAGTCGCGatat                            >  1:582257/1‑140 (MQ=255)                                                                                                          gCCAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGCGCCGCTTCGACGGAACCATATTGCTGATAGGTCGCGATAtt                           <  1:773674/140‑1 (MQ=255)                                                                                                           ccAGGATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGCGCCGCTTCGACGGAACCATATTGCTGATAGGTCGCGATAttt                          >  2:329075/1‑140 (MQ=255)                                                                                                               gATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGCGCCGCTTCGACGGAACCATATTGCTGATAGGTcg                                  <  1:880672/128‑1 (MQ=255)                                                                                                               gATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGCGCCGCTTCGACGGAACCATATTGCTGATAGGTcg                                  >  2:880672/1‑128 (MQ=255)                                                                                                               gATCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGCGCCGCTTCGACGGAACCATATTGCTGATAGGTCGCGATATTTTCCa                      >  1:312921/1‑140 (MQ=255)                                                                                                                aTCAGGCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGCGCCGCTTCGACGGAACCATATTGCTGATAGGTCGCGATATTTTCCAg                     >  1:480368/1‑140 (MQ=255)                                                                                                                    ggCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGCGCCGCTTCGACGGAACCATATTGCTGATAGGTCGCGATATTTTCCAGTGcc                 >  2:71190/1‑140 (MQ=255)                                                                                                                    ggCCTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGCGCCGCTTCGACGGAACCATATTGCTGATAGGTCGCGATATTTTCCAGTGcc                 >  1:337094/1‑140 (MQ=255)                                                                                                                     gCCTAAAGTCGCACCCCCGCCCCAAAGGAAAATAAAGGAGCCCAGCATCGGCTTGGCCCAGATATGGAAGGTC‑TACCGGCTGCCAGCGCCGCTTCGACGGAACCATATTGCTGATAGGTCGCGATATTTTCCAGTGCCCa               <  1:234705/140‑1 (MQ=255)                                                                                                                      ccTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGCGCCGCTTCGACGGAACCATATTGCTGATAGGTCGCGATATTTTCCAGTGCCCa               <  2:582257/140‑1 (MQ=255)                                                                                                                      ccTAAAGTCGCACCACCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGCGCCGCTTCGACGGAACCATATTGCTGATAGGTCGCGATATTTTCCAGTGCCCa               <  2:312921/140‑1 (MQ=255)                                                                                                                                caccacCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGCGCCGCTTCGACGGAACCATATTGCTGATAGGTCGCGATATTTTCCAGTGCCCACgg            <  1:362713/133‑1 (MQ=255)                                                                                                                                caccacCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGCGCCGCTTCGACGGAACCATATTGCTGATAGGTCGCGATATTTTCCAGTGCCCACgg            >  2:362713/1‑133 (MQ=255)                                                                                                                                caccacCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGCGCCGCTTCGACGGAACCATATTGCTGATAGGTCGCGATATTTTCCAGTGCCCACGGCg          <  2:422322/135‑1 (MQ=255)                                                                                                                                caccacCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGCGCCGCTTCGACGGAACCATATTGCTGATAGGTCGCGATATTTTCCAGTGCCCACGGCg          >  1:422322/1‑135 (MQ=255)                                                                                                                                   cacCGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGCGCCGCTTCGACGGAACCATATTGCTGATAGGTCGCGATATTTTCCAGTGCCCACGGCGTCATAATg  <  2:480368/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         ATTGGCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACTGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGCGCCGCTTCGACGGAACCATATTGCTGATAGGTCGCGATATTTTCCAGTGCCCACGGCGTCATAATG  >  NC_000913/1820163‑1820432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑