Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,889,776	T→C	intergenic (‑30/+175)	fadD ← / ← yeaY	fatty acyl‑CoA synthetase/Slp family lipoprotein YeaY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,889,776	0	T	C	100.0%	247.2 / NA	67	intergenic (‑30/+175)	fadD/yeaY	fatty acyl‑CoA synthetase/Slp family lipoprotein YeaY
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/40);  total (27/40)

GTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGTCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTA  >  NC_000913/1889639‑1889896                                                                                                                                          |                                                                                                                         gTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCa                                                                                                                        >  2:1125494/1‑140 (MQ=255)    gcgcgcGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCCCTAAAATGCGTGTTCGTCGCCATCg                                                                                                                     <  1:1097827/140‑1 (MQ=255)     cgcgcgACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGc                                                                                                                    <  1:363143/140‑1 (MQ=255)      gcgcgACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCa                                                                                                                   <  2:249305/140‑1 (MQ=255)       cgcgACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCaa                                                                                                                  <  1:804472/140‑1 (MQ=255)          gaccgacTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAAccc                                                                                                               >  1:1067133/1‑140 (MQ=255)          gaccgacTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAAccc                                                                                                               >  1:429188/1‑140 (MQ=255)          gaccgacTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAAccc                                                                                                               >  2:161658/1‑140 (MQ=255)          gaccgacTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAAccc                                                                                                               >  1:272792/1‑140 (MQ=255)           accgacTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAAcccc                                                                                                              <  1:1055733/140‑1 (MQ=255)           accgacTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAAcccc                                                                                                              <  2:429188/140‑1 (MQ=255)           accgacTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAAcccc                                                                                                              <  1:298764/140‑1 (MQ=255)           accgacTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAAcccc                                                                                                              <  1:845771/140‑1 (MQ=255)           accgacTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAAcccc                                                                                                              <  2:854722/140‑1 (MQ=255)           accgacTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAAcccc                                                                                                              <  2:1067133/140‑1 (MQ=255)           accgacTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAAcccc                                                                                                              <  1:1125494/140‑1 (MQ=255)            ccgacTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCa                                                                                                             <  1:431502/140‑1 (MQ=255)             cgacTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCaa                                                                                                            <  2:671199/140‑1 (MQ=255)                cTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATg                                                                                                         <  2:660766/140‑1 (MQ=255)                 tGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGa                                                                                                        <  1:832793/140‑1 (MQ=255)                      aaaCATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATAc                                                                                                   >  2:815701/1‑140 (MQ=255)                           tatCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCACCCCCAAATGATATACATGcc                                                                                              <  2:225557/140‑1 (MQ=255)                             tCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCACCCCCAAATGATATACATGCCGt                                                                                            <  1:161658/140‑1 (MQ=255)                             tCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCTCAAATGATATACATGCCGt                                                                                            >  2:800327/1‑140 (MQ=255)                             tCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGt                                                                                            >  2:300289/1‑140 (MQ=255)                             tCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGt                                                                                            <  1:815701/140‑1 (MQ=255)                             tCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGt                                                                                            >  2:747907/1‑140 (MQ=255)                             tCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGt                                                                                            >  1:655327/1‑140 (MQ=255)                             tCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGt                                                                                            <  2:138110/140‑1 (MQ=255)                             tCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGt                                                                                            >  1:138110/1‑140 (MQ=255)                              cTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGtt                                                                                           >  1:792712/1‑140 (MQ=255)                                  cAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCACCCCCAAATGATATACATGCCGTTAACa                                                                                       <  2:730951/140‑1 (MQ=255)                                  cAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGTTAACa                                                                                       >  2:342052/1‑140 (MQ=255)                                   agagATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGTTAACat                                                                                      <  1:8371/140‑1 (MQ=255)                                   agagATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGTTAACat                                                                                      <  1:498730/140‑1 (MQ=255)                                   agagATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCAATCGCAACCCAAAATGATATCCATGCCGTTAACat                                                                                      <  2:1111686/140‑1 (MQ=255)                                     agaTTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGt                                                                                            <  1:653058/132‑1 (MQ=255)                                     agaTTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGt                                                                                            >  2:653058/1‑132 (MQ=255)                                     agaTTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGTTAACataa                                                                                    >  2:842167/1‑140 (MQ=255)                                      gaTTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGTTAACataat                                                                                   >  1:547576/1‑140 (MQ=255)                                       aTTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGTTAACa                                                                                       >  2:861140/1‑135 (MQ=255)                                       aTTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGTTAACa                                                                                       <  1:861140/135‑1 (MQ=255)                                           aTAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCTCAAATGATATACATGCCGTTAACATAATATTaa                                                                              <  1:800327/140‑1 (MQ=255)                                           aTAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTaa                                                                              <  1:747907/140‑1 (MQ=255)                                           aTAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTaa                                                                              <  2:547576/140‑1 (MQ=255)                                           aTAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTaa                                                                              <  1:300289/140‑1 (MQ=255)                                           aTAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCAAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCACCCCCAAATGATATACATGCCGTTAACATAATATTaa                                                                              <  2:564264/140‑1 (MQ=255)                                               cGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACtca                                                                          >  1:431528/1‑140 (MQ=255)                                                 gTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACtcatc                                                                        >  2:189435/1‑140 (MQ=255)                                                 gTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACtcatc                                                                        >  1:197475/1‑140 (MQ=255)                                                    aGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATa                                                                     <  1:189250/140‑1 (MQ=255)                                                     gggTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATAc                                                                    <  1:189435/140‑1 (MQ=255)                                                     gggTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATAc                                                                    <  2:792712/140‑1 (MQ=255)                                                         tGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGc                                                                <  1:718456/140‑1 (MQ=255)                                                         tGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCAAAACCTTCTTCAATTCTTCCCCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGc                                                                <  2:114283/140‑1 (MQ=255)                                                           aTCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCtt                                                              >  1:755480/1‑140 (MQ=255)                                                                cGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATaa                                                         <  1:1049825/140‑1 (MQ=255)                                                                      aaCGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTAccc                                                   <  2:755480/140‑1 (MQ=255)                                                                                     aCGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCg                                    <  1:842167/140‑1 (MQ=255)                                                                                           aaGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAgcgc                              >  1:57485/1‑140 (MQ=255)                                                                                               cAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATATATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCa                          <  1:51177/140‑1 (MQ=255)                                                                                                 aaCCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACt                        >  1:857466/1‑140 (MQ=255)                                                                                                  aCCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACta                       >  2:501699/1‑140 (MQ=255)                                                                                                   ccTTCTTCAATTTTTCACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACtat                      <  2:655327/140‑1 (MQ=255)                                                                                                                ttcACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCtt         >  1:497062/1‑140 (MQ=255)                                                                                                                ttcACCTCTAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCtt         >  1:261465/1‑140 (MQ=255)                                                                                                                      tctAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGt   <  2:431528/140‑1 (MQ=255)                                                                                                                       ctAAAATGCGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTa  <  2:57485/140‑1 (MQ=255)                                                                                                                               cGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGt   <  2:644805/131‑1 (MQ=255)                                                                                                                               cGTGTTCGTCGCCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGt   >  1:644805/1‑131 (MQ=255)                                                                                                                                          |                                                                                                                         GTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTCGTCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTA  >  NC_000913/1889639‑1889896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑