Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,902,492	G→A	A149T (GCT→ACT)	manX →	mannose‑specific PTS enzyme IIAB component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,902,492	0	G	A	100.0%	153.2 / NA	45	A149T (GCT→ACT)	manX	mannose‑specific PTS enzyme IIAB component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/23);  total (22/23)

TGATGATGACCCAAGCTTTGATGAACTGGTGGCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGGCTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTGCGCGTATCGACGACCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGAAACCAATGTCTCCCGTATTATT  >  NC_000913/1902362‑1902623                                                                                                                                   |                                                                                                                                    tgatgatgaCCCAAGCTTTGATGAACTGGTGGCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTc                                                                                                                            >  1:722007/1‑140 (MQ=255)        gaCCCAAGCTTTGATGAACTGGTGGCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCaaa                                                                                                                     >  1:31108/1‑140 (MQ=255)              aGCTTTGATGAACTGGTGGCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAAt                                                                                                               <  1:254703/140‑1 (MQ=255)              aGCTTTGATGAACTGGTGGCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAAt                                                                                                               >  1:72821/1‑140 (MQ=255)               gCTTTGATGAACTGGTGGCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATg                                                                                                              >  1:982966/1‑140 (MQ=255)                       gaACTGGTGGCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCaaa                                                                                                      <  2:31108/140‑1 (MQ=255)                       gaACTGGTGGCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCaaa                                                                                                      <  1:247639/140‑1 (MQ=255)                       gaACTGGTGGCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCAGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCaaa                                                                                                      <  1:858638/140‑1 (MQ=255)                          cTGGTGGCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGa                                                                                                   <  1:975030/140‑1 (MQ=255)                          cTGGTGGCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGa                                                                                                   <  1:946/140‑1 (MQ=255)                           tggtggCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGAc                                                                                                  >  2:799254/1‑140 (MQ=255)                             gtggCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCaa                                                                                                                >  2:678454/1‑124 (MQ=255)                             gtggCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCaa                                                                                                                <  1:678454/124‑1 (MQ=255)                             gtggCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTa                                                                                                >  2:606995/1‑140 (MQ=255)                             gtggCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTa                                                                                                >  1:825917/1‑140 (MQ=255)                             gtggCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTa                                                                                                >  1:321539/1‑140 (MQ=255)                              tggCACTGGCAGTACAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTAc                                                                                               <  2:982966/140‑1 (MQ=255)                               ggCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACa                                                                                              <  1:771231/140‑1 (MQ=255)                               ggCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACa                                                                                              <  1:606995/140‑1 (MQ=255)                               ggCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACa                                                                                              <  2:72821/140‑1 (MQ=255)                                gCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACAt                                                                                             <  2:321539/140‑1 (MQ=255)                                gCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACAt                                                                                             >  2:473075/1‑140 (MQ=255)                                   cTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACATGGt                                                                                          <  1:473075/140‑1 (MQ=255)                                    tGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACa                                                                                              <  2:1049445/135‑1 (MQ=255)                                    tGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACa                                                                                              >  1:1049445/1‑135 (MQ=255)                                       cAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTAtt                                                                                      >  2:319428/1‑140 (MQ=255)                                       cAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTAtt                                                                                      >  2:290393/1‑140 (MQ=255)                                          tAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTATTGGc                                                                                   >  2:180839/1‑140 (MQ=255)                                                cAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTgcg                                                                             >  2:265485/1‑140 (MQ=255)                                                 aGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTgcgc                                                                            <  2:273542/140‑1 (MQ=255)                                                 aGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTgcgc                                                                            >  2:253536/1‑140 (MQ=255)                                                  ggCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAA‑GGGGCCAAACGACTACATGGTTATTGGCCTTGCGCGt                                                                          >  2:312988/1‑140 (MQ=255)                                                    ccGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTGCGCGTa                                                                         >  1:763633/1‑140 (MQ=255)                                                       tGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGACAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTGCGCGTATcg                                                                      >  1:954387/1‑140 (MQ=255)                                                       tGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAAATCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTGCGCGTATcg                                                                      >  2:355600/1‑140 (MQ=255)                                                        gAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTGAGCGTATcga                                                                     <  2:214347/140‑1 (MQ=255)                                                         aaGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTGCGCGTATcgac                                                                    >  1:938357/1‑140 (MQ=255)                                                                gAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAATTCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTGCGCGTATCGACGACCGTc                                                             <  2:998179/140‑1 (MQ=255)                                                                 aaaGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTGCGCGTATCGACGACCGTCt                                                            <  1:253536/140‑1 (MQ=255)                                                                     cACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTGCGCGTATCGACGACCGTCTGAtt                                                        <  2:938357/140‑1 (MQ=255)                                                                               cAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGAAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTGCGCGTATCGACGACCGTCTGATTCACGGTCAgg                                              <  2:574475/140‑1 (MQ=255)                                                                                         gAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTGCGCGTATCGACGACCGTCTGATTCACGGTCAGGTCGCCACCCg                                    <  1:799254/140‑1 (MQ=255)                                                                                         gAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGACAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTGCGCGTATCGACGACCGTCTGATTCACGGTCAGGTCGCCACCCg                                    <  2:954387/140‑1 (MQ=255)                                                                                                       aGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTGCGCGTATCGACGACCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGaaa                      >  2:1073421/1‑140 (MQ=255)                                                                                                                      agcaCCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTGCGCGTATCGACGACCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGAAACCAATGTCTCCCGta       <  2:834176/140‑1 (MQ=255)                                                                                                                         aCCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTGCGCGTATCGACGACCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGAAACCAATGTCTCCCGtatta    <  1:265485/140‑1 (MQ=255)                                                                                                                           cAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTGCGCGTATCGACGACCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGAAACCAATGTCTCCCGTattatt  <  1:149893/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                    TGATGATGACCCAAGCTTTGATGAACTGGTGGCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGGCTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTGCGCGTATCGACGACCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGAAACCAATGTCTCCCGTATTATT  >  NC_000913/1902362‑1902623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑