Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,948,451	A→G	V61A (GTC→GCC)	nudB ←	dihydroneopterin triphosphate diphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,948,451	0	A	G	100.0%	144.9 / NA	40	V61A (GTC→GCC)	nudB	dihydroneopterin triphosphate diphosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/16);  total (24/16)

CGCAAGACAGAACCATGATTCCGTATTACGCGTCACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGACCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCACGCCGCTGCAACATCAGCACCCGTTTCGTATCTTGTGCGTAGAT  >  NC_000913/1948315‑1948587                                                                                                                                         |                                                                                                                                         cgcAAGACAGAACCATGATTCCGTATTACGCGTCACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCct                                                                                                                                       <  1:964749/140‑1 (MQ=255)      gACAGAACCATGATTCCGTATTACGCGTCACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTcc                                                                                                                                  >  1:1132961/1‑140 (MQ=255)           aaCCATGATTCCGTATTACGCGTCACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTAc                                                                                                                             >  1:1078653/1‑140 (MQ=255)                tGATTCCGTATTACGCGTCACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTcgc                                                                                                                        >  2:79128/1‑140 (MQ=255)                tGATTCCGTATTACGCGTCACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTcgc                                                                                                                        >  2:368476/1‑140 (MQ=255)                 gATTCCGTATTACGCGTCACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTcgcg                                                                                                                       <  1:79128/140‑1 (MQ=255)                 gATTCCGTATTACGCGTCACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTcgcg                                                                                                                       <  2:1050141/140‑1 (MQ=255)                     ccGTATTACGCGTCACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATg                                                                                                                   >  2:591420/1‑140 (MQ=255)                             cgcgTCACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTg                                                                                                           >  1:225709/1‑140 (MQ=255)                             cgcgTCACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTg                                                                                                           >  2:506168/1‑140 (MQ=255)                             cgcgTCACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTg                                                                                                           >  2:161464/1‑140 (MQ=255)                                gTCACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTgcgg                                                                                                        >  1:143832/1‑140 (MQ=255)                                  cACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTgcggcg                                                                                                      <  1:298931/140‑1 (MQ=255)                                  cACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTgcggcg                                                                                                      <  2:466131/140‑1 (MQ=255)                                  cACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTgcggcg                                                                                                      >  1:540649/1‑140 (MQ=255)                                       ccGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGtt                                                                                                 <  1:591420/140‑1 (MQ=255)                                          gcgcATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTgcggcg                                                                                                      <  2:666745/132‑1 (MQ=255)                                          gcgcATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTgcggcg                                                                                                      >  1:666745/1‑132 (MQ=255)                                                  cGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTc                                                                                      <  1:495135/140‑1 (MQ=255)                                                                  aaaaaaTTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCACTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCa                                                                                    <  1:135217/126‑1 (MQ=255)                                                                  aaaaaaTTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCACTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCa                                                                                    >  2:135217/1‑126 (MQ=255)                                                                    aaaaTTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGa                                                                    <  1:161464/140‑1 (MQ=255)                                                                                          gcTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGaa                                                           <  1:264648/127‑1 (MQ=255)                                                                                          gcTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGaa                                                           >  2:264648/1‑127 (MQ=255)                                                                                          gcTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCa                                              >  2:1102916/1‑140 (MQ=255)                                                                                          gcTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCa                                              >  1:293895/1‑140 (MQ=255)                                                                                          gcTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCa                                              >  1:331815/1‑140 (MQ=255)                                                                                                    aaTTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCACGCCGCTGCa                                    <  1:881772/140‑1 (MQ=255)                                                                                                          ggTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCACGCCGCTGCAACATCa                              >  2:221259/1‑140 (MQ=255)                                                                                                              aGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCACGCCGCTGCAACATCAGCAc                          >  2:1118716/1‑140 (MQ=255)                                                                                                                ttgttCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCACGCCGCTGCAACATCAGCAccc                        >  2:333921/1‑140 (MQ=255)                                                                                                                  gttCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCACGCCGCTGCAACATCAGCACCCGt                      <  2:723737/140‑1 (MQ=255)                                                                                                                     cAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCACGCCGCTGCAACATCAGCACCCGTTTc                   >  2:224054/1‑140 (MQ=255)                                                                                                                             acaTCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCACGCCGCTGCAACATCAGCACCCGTTTCGTATCTtg           >  1:534956/1‑140 (MQ=255)                                                                                                                             acaTCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGCTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCACGCCGCTGCAACATCAGCACCCGTTTCGTATCTtg           >  1:189214/1‑140 (MQ=255)                                                                                                                               aTCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCACGCCGCTGCAACATCAGCACCCGTTTCGTATCTtgtg         <  1:676209/140‑1 (MQ=255)                                                                                                                               aTCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCACGCCGCTGCAACATCAGCACCCGTTTCGTATCTtgtg         <  1:221259/140‑1 (MQ=255)                                                                                                                                  aaTGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCACGCCGCTGCAACATCAGCACCCGTTTCGTATCTTGTGCGt      >  2:484129/1‑140 (MQ=255)                                                                                                                                   aTGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCACGCCGCTGCAACATCAGCACCCGTTTCGTATCTTGTGCGTa     <  2:126959/140‑1 (MQ=255)                                                                                                                                      gTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCACGCCGCTGCAACATCAGCACCCGTTTCGTATCTTGTGCGTAGAt  >  1:655044/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         CGCAAGACAGAACCATGATTCCGTATTACGCGTCACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGACCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCACGCCGCTGCAACATCAGCACCCGTTTCGTATCTTGTGCGTAGAT  >  NC_000913/1948315‑1948587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑