Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,956,138	T→C	K290K (AAA→AAG)	torZ ←	trimethylamine N‑oxide reductase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,956,138	0	T	C	100.0%	205.4 / NA	55	K290K (AAA→AAG)	torZ	trimethylamine N‑oxide reductase 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/32);  total (23/32)

CTGCGCTTCCGGTACGCCAGTAATTTCCGCCGCCCAGACCGCGCTCTTCGGCGTATTATCGCTCTTACCTGTCAGATACTCTTCAAACTGCGGATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGTTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCATATTCGGCGCGATCCAGGTGGCATTATCGTCAAAGAATTCGATGGTTTCGGAGCGGATAGGATCAATGGCAATCACT  >  NC_000913/1956006‑1956273                                                                                                                                     |                                                                                                                                        cTGCGCTTCCGGTACGCCAGTAATTTCCGCCGCCCAGACCGCGCTCTTCGGCGTATTATCGCTCTTACCTGTCAGATACTCTTCAAACTGCGGATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCtt                                                                                                                                  >  2:46807/1‑140 (MQ=255)  tGCGCTTCCGGTACGCCAGTAATTTCCGCCGCCCAGACCGCGCTCTTCGGCGTATTATCGCTCTTACCTGTCAGATACTCTTCAAACTGCGGATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTtg                                                                                                                                 >  1:875659/1‑140 (MQ=255)    cgcTTCCGGTACGCCAGTAATTTCCGCCGCCCAGACCGCGCTCTTCGGCGTATTATCGCTCTTACCTGTCAGATACTCTTCAAACTGCGGATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTtgtg                                                                                                                               <  1:221191/140‑1 (MQ=255)        tCCGGTACGCCAGTAATTTCCGCCGCCCAGACCGCGCTCTTCGGCGTATTATCGCTCTTACCTGTCAGATACTCTTCAAACTGCGGATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGtcat                                                                                                                           <  1:1060950/140‑1 (MQ=255)         ccGGTACGCCAGTAATTTCCGCCGCCCAGACCGCGCTCTTCGGCGTATTATCGCTCTTACCTGTCAGATACTCTTCAAACTGCGGATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGtcatc                                                                                                                          >  2:660626/1‑140 (MQ=255)         ccGGTACGCCAGTAATTTCCGCCGCCCAGACCGCGCTCTTCGGCGTATTATCGCTCTTACCTGTCAGATACTCTTCAAACTGCGGATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGtcatc                                                                                                                          <  1:404595/140‑1 (MQ=255)          cGGTACGCCAGTAATTTCCGCCGCCCAGACCGCGCTCTTCGGCGTATTATCGCTCTTACCTGTCAGATACTCTTCAAACTGCGGATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGtcatca                                                                                                                         >  1:883330/1‑140 (MQ=255)            gTACGCCAGTAATTTCCGCCGCCCAGACCGCGCTCTTCGGCGTATTATCGCTCTTACCTGTCAGATACTCTTCAAACTGCGGATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAgg                                                                                                                       >  1:1076387/1‑140 (MQ=255)                   aGTAATTTCCGCCGCCCAGACCGCGCTCTTCGGCGTATTATCGCTCTTACCTGTCAGATACTCTTCAAACTGCGGATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATgcg                                                                                                                <  2:875659/140‑1 (MQ=255)                   aGTAATTTCCGCCGCCCAGACCGCGCTCTTCGGCGTATTATCGCTCTTACCTGTCAGATACTCTTCAAACTGCGGATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATgcg                                                                                                                <  2:719752/140‑1 (MQ=255)                     tAATTTCCGCCGCCCAGACCGCGCTCTTCGGCGTATTATCGCTCTTACCTGTCAGATACTCTTCAAACTGCGGATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCa                                                                                                              <  1:10078/140‑1 (MQ=255)                     tAATTTCCGCCGCCCAGACCGCGCTCTTCGGCGTATTATCGCTCTTACCTGTCAGATACTCTTCAAACTGCGGATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCa                                                                                                              <  1:117560/140‑1 (MQ=255)                      aaTTTCCGCCGCCCAGACCGCGCTCTTCGGCGTATTATCGCTCTTACCTGTCAGATACTCTTCAAACTGCGGATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCaa                                                                                                             <  1:46807/140‑1 (MQ=255)                         ttCCGCCGCCCAGACCGCGCTCTTCGGCGTATTATCGCTCTTACCTGTCAGATACTCTTCAAACTGCGGATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATcc                                                                                                          >  2:270777/1‑140 (MQ=255)                              ccgccCAGACCGCGCTCTTCGGCGTATTATCGCTCTTACCTGTCAGATACTCTTCAAACTGCGGATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAAc                                                                                                     >  2:913514/1‑140 (MQ=255)                                gccCAGACCGCGCTCTTCGGCGTATTATCGCTCTTACCTGTCAGATACTCTTCAAACTGCGGATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACAt                                                                                                   <  2:1121989/140‑1 (MQ=255)                                           gcTCTTCGGCGTATTATCGCTCTTACCTGTCAGATACTCTTCAAACTGCGGATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGt                                                                                        >  1:242797/1‑140 (MQ=255)                                              ctTCGGCGTATTATCGCTCTTACCTGTCAGATACTCTTCAAACTGCGGATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCgg                                                                                     <  2:242797/140‑1 (MQ=255)                                                    cGTATTATCGCTCTTACCTGTCAGATACTCTTCAAACTGCGGATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCa                                                                               >  2:613282/1‑140 (MQ=255)                                                         tatCGCTCTTACCTGTCAGATACTCTTCAAACTGCGGATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCATATTc                                                                          >  2:490213/1‑140 (MQ=255)                                                           tCGCTCTTACCTGTCAGATACTCTTCAAACTGCGGATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTGACATCAGTGCCACGTCGGTGCCCATATTCgg                                                                        <  2:188271/140‑1 (MQ=255)                                                             gCTCTTACCTGTCAGATACTCTTCAAACTGCGGATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCATATTCGgcg                                                                      <  2:883330/140‑1 (MQ=255)                                                             gCTCTTACCTGTCAGATACTCTTCAAACTGCGGATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCATATTCGgcg                                                                      <  2:1076387/140‑1 (MQ=255)                                                                  tACCTGTCAGATACTCTTCAAACTGCGGATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCATATTCGGCGCGATc                                                                 <  2:1075287/140‑1 (MQ=255)                                                                   aCCTGTCAGATACTCTTCAAACTGCGGATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCATATTCGGCGCGa                                                                   >  1:1041835/1‑137 (MQ=255)                                                                   aCCTGTCAGATACTCTTCAAACTGCGGATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCATATTCGGCGCGa                                                                   <  2:1041835/137‑1 (MQ=255)                                                                   aCCTGTCAGATACTCTTCAAACTGCGGATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGGATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCATATTCGGCGCGATcc                                                                >  1:73887/1‑140 (MQ=255)                                                                       gTCAGATACTCTTCAAACTGCGGATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCACATATTCGGCGCGATCCAGGt                                                            <  1:758686/140‑1 (MQ=255)                                                                        tCAGATACTCTTCAAACTGCGGATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCATATTCGGCGCGATCCAGGTg                                                           >  1:939967/1‑140 (MQ=255)                                                                         cAGATACTCTTCAAACTGCGGATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCATATTCGGCGCGATCCAGGTgg                                                          >  1:674334/1‑140 (MQ=255)                                                                           gATACTCTTCAAACTGCGGATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCATATTCGGCGCGATCCAGGTGGCa                                                        >  2:384939/1‑140 (MQ=255)                                                                            aTACTCTTCAAACTGCGGATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCATATTCGGCGCGATCCAGGTGGCAt                                                       <  2:328616/140‑1 (MQ=255)                                                                                tctTCAAACTGCGGATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCATATTCGGCGCGATCCAGGTGGCATTATc                                                   >  2:150256/1‑140 (MQ=255)                                                                                 ctTCAAACTGCGGATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCATATTCGGCGCGATCCAGGTGGCATTATCg                                                  <  1:29662/140‑1 (MQ=255)                                                                                  ttCAAACTGCGGATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCATATTCGGCGCGATCCAGGTGGCATTATCGt                                                 >  1:922073/1‑140 (MQ=255)                                                                                  ttCAAACTGCGGATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCATATTCGGCGCGATCCAGGTGGCATTATCGt                                                 >  1:434159/1‑140 (MQ=255)                                                                                      aaCTGCGGATACCCGGTAGTGTATTTTTCAAGGAACCCTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCATATTCGGCGCGATCCAGGTGGCATTATCGTCaaa                                             <  2:73887/140‑1 (MQ=255)                                                                                            ggATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCATATTCGGCGCGATCCAGGTGGCATTATCGTCAAAGAATTc                                       >  1:550109/1‑140 (MQ=255)                                                                                              aTACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCATATTCGGCGCGATCCAGGTGGCATTATCGTCAAAGAATTCGa                                     <  1:195799/140‑1 (MQ=255)                                                                                                   cGGTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCATATTCGGCGCGATCCAGGTGGCATTATCGTCAAAGAATTCGATGGtt                                <  1:151745/140‑1 (MQ=255)                                                                                                    ggTAGTGTATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCATATTCGGCGCGATCCAGGTGGCATTATCGTCAAAGAATTCGATGGttt                               <  1:384939/140‑1 (MQ=255)                                                                                                        gtgtATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCATATTCGGCGCGATCCAGGTGGCATTATCGTCAAAGAATTCGATGGTTTCGGa                           <  2:939967/140‑1 (MQ=255)                                                                                                        gtgtATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCATATTCGGCGCGATCCAGGTGGCATTATCGTCAAAGAATTCGATGGTTTCGGa                           <  1:843842/140‑1 (MQ=255)                                                                                                         tgtATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCATATTCGGCGCGATCCAGGTGGCATTATCGTCAAAGAATTCGATGGTTTCGGAg                          <  1:913514/140‑1 (MQ=255)                                                                                                         tgtATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCATATTCGGCGCGATCCAGGTGGCATTATCGTCAAAGAATTCGATGGTTTCGGAg                          <  1:613282/140‑1 (MQ=255)                                                                                                          gtATTTTTCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCATATTCGGCGCGATCCAGGTGGCATTATCGTCAAAGAATTCGATGGTTTCGGAGc                         >  2:739487/1‑140 (MQ=255)                                                                                                              ttttCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCATATTCGGCGCGATCCAGGTGGCATTATCGTCAAAGAATTCGATGGTTTCGGAGCGGAt                     <  2:187723/140‑1 (MQ=255)                                                                                                               tttCAAGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCATATTCGGCGCGATCCAGGTGGCATTATCGTCAAAGAATTCGATGGTTTCGGAGCGGATa                    <  1:967459/140‑1 (MQ=255)                                                                                                                   aaGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCATATTCGGCGCGATCCAGGTGGCATTATCGTCAAAGAATTCGATGGTTTCGGAg                          >  2:842019/1‑130 (MQ=255)                                                                                                                   aaGGAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCATATTCGGCGCGATCCAGGTGGCATTATCGTCAAAGAATTCGATGGTTTCGGAg                          <  1:842019/130‑1 (MQ=255)                                                                                                                     ggAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCATATTCGGCGCGATCCAGGTGGCATTATCGTCAAAGAATTCGATGGTTTCGGAGCGGAt                     >  2:766806/1‑133 (MQ=255)                                                                                                                     ggAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCATATTCGGCGCGATCCAGGTGGCATTATCGTCAAAGAATTCGATGGTTTCGGAGCGGAt                     <  1:766806/133‑1 (MQ=255)                                                                                                                     ggAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCATATTCGGCGCGATCCAGGTGGCATTATCGTCAAAGAATTCGATGGTTTCGGAGCGGAt                     <  1:108032/133‑1 (MQ=255)                                                                                                                     ggAACACTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCATATTCGGCGCGATCCAGGTGGCATTATCGTCAAAGAATTCGATGGTTTCGGAGCGGAt                     >  2:108032/1‑133 (MQ=255)                                                                                                                         cacTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCATATTCGGCGCGATCCAGGTGGCATTATCGTCAAAGAATTCGATGGTTTCGGAGCGGATAGGATCAATgg          <  2:674334/140‑1 (MQ=255)                                                                                                                          acTTTATCGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCATATTCGGCGCGATCCAGGTGGCATTATCGTCAAAGAATTCGATGGTTTCGGAGCGGATAGGATCAATGGc         <  1:490213/140‑1 (MQ=255)                                                                                                                                 cGTGCTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCATATTCGGCGCGATCCAGGTGGCATTATCGTCAAAGAATTCGATGGTTTCGGAGCGGATAGGATCAATGGCAATCACt  >  1:399306/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                        CTGCGCTTCCGGTACGCCAGTAATTTCCGCCGCCCAGACCGCGCTCTTCGGCGTATTATCGCTCTTACCTGTCAGATACTCTTCAAACTGCGGATACCCGGTAGTGTATTTTTCAAGGAACACTTTATCGTGTTTGCCTTGTGTCATCAGGGTATGCGCAATCCCTAACATCAGTGCCACGTCGGTGCCCATATTCGGCGCGATCCAGGTGGCATTATCGTCAAAGAATTCGATGGTTTCGGAGCGGATAGGATCAATGGCAATCACT  >  NC_000913/1956006‑1956273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑