Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,080,189	T→C	T135A (ACA→GCA)	yeeA ←	putative transporter YeeA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,080,189	0	T	C	100.0%	201.7 / NA	55	T135A (ACA→GCA)	yeeA	putative transporter YeeA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/30);  total (25/30)

GGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGTCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTA  >  NC_000913/2080055‑2080320                                                                                                                                       |                                                                                                                                    ggTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCgggg                                                                                                                                <  2:808983/140‑1 (MQ=255) ggTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCgggg                                                                                                                                <  2:1022102/140‑1 (MQ=255) ggTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCgggg                                                                                                                                <  2:488454/140‑1 (MQ=255) ggTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCgggg                                                                                                                                <  1:410312/140‑1 (MQ=255)   tCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGaa                                                                                                                              >  2:90527/1‑140 (MQ=255)   tCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGaa                                                                                                                              >  1:790837/1‑140 (MQ=255)   tCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGaa                                                                                                                              >  1:338641/1‑140 (MQ=255)     aGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAAcc                                                                                                                            <  2:1085301/140‑1 (MQ=255)        cTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCcac                                                                                                                         <  1:826991/140‑1 (MQ=255)                 aGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGc                                                                                                                <  2:338641/140‑1 (MQ=255)                 aGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGc                                                                                                                <  2:42425/140‑1 (MQ=255)                  gTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGTTCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGcc                                                                                                               <  2:345276/140‑1 (MQ=255)                  gTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGcc                                                                                                               <  1:939732/140‑1 (MQ=255)                      aaTGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCAGCg                                                                                                           <  2:353029/140‑1 (MQ=255)                             cAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCAcccc                                                                                                    <  1:655705/140‑1 (MQ=255)                             cAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCCTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCAcccc                                                                                                    <  2:167697/140‑1 (MQ=255)                             cAGTGGATGAACGCCCGTTGTGGCAAGATACCGGTAAACAACATTGCCAATAAAGAGCCGAGGGACACATCGCCGCTTCGCCATCACACCGTATCAATTTCACCTGCAGAGCAACCCACAACAATTGCCAGCGTCatcgc                                                                                                    >  2:7726/1‑136 (MQ=255)                                       aCGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCGGCGTCACCCCAATCAATaaa                                                                                          >  2:996961/1‑140 (MQ=255)                                       aCGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATaaa                                                                                          <  2:790837/140‑1 (MQ=255)                                          cccGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCt                                                                                       <  2:1081737/140‑1 (MQ=255)                                           ccGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCtt                                                                                      >  2:15646/1‑140 (MQ=255)                                            cGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTg                                                                                     >  2:896023/1‑140 (MQ=255)                                                 tgGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCGGCGTCACCCCAATCAATAAACCTTGATACg                                                                                <  1:996961/140‑1 (MQ=255)                                                   gCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGt                                                                              >  2:403534/1‑140 (MQ=255)                                                           ccGGTAAACAACATTGCCAGTAAAGAGCCGAGGGTCACATCGCGGCTTCGCAATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCAGCCCAATCAATAAACCTTGATACGGTTTCTTGcc                                                                      >  2:1129711/1‑140 (MQ=255)                                                           ccGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGcc                                                                      <  1:72446/140‑1 (MQ=255)                                                               tAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAgc                                                                  >  2:173089/1‑140 (MQ=255)                                                                  acaacaTTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCgcc                                                               >  2:735075/1‑140 (MQ=255)                                                                      caTTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCgccagcc                                                           >  2:1070859/1‑140 (MQ=255)                                                                         tGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAAc                                                        <  1:896023/140‑1 (MQ=255)                                                                         tGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAAc                                                        >  1:923025/1‑140 (MQ=255)                                                                         tGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAAc                                                        >  2:233006/1‑140 (MQ=255)                                                                           ccAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCg                                                      >  2:29370/1‑140 (MQ=255)                                                                              gTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCaa                                                   >  1:332275/1‑140 (MQ=255)                                                                              gTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCaa                                                   <  1:403534/140‑1 (MQ=255)                                                                                      ccGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACAt                                           >  1:37346/1‑140 (MQ=255)                                                                                       cGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATg                                          >  1:531923/1‑140 (MQ=255)                                                                                        gAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATgg                                         >  1:994996/1‑140 (MQ=255)                                                                                         aGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGgc                                        <  1:173089/140‑1 (MQ=255)                                                                                           gATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGgccg                                      >  1:609652/1‑140 (MQ=255)                                                                                                caTCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCcgcg                                 >  1:1002611/1‑140 (MQ=255)                                                                                                  tCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCa                               <  1:735075/140‑1 (MQ=255)                                                                                                   cgccgcTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCAc                              <  2:923025/140‑1 (MQ=255)                                                                                                     ccgcTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGccgccg                                   >  2:361552/1‑133 (MQ=255)                                                                                                     ccgcTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGccgccg                                   <  1:361552/133‑1 (MQ=255)                                                                                                     ccgcTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCAc                              <  1:777997/138‑1 (MQ=255)                                                                                                     ccgcTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCAc                              >  2:777997/1‑138 (MQ=255)                                                                                                      cgcTTCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAg                           <  1:1093518/140‑1 (MQ=255)                                                                                                          tCGCCATAACGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTa                       >  2:212546/1‑140 (MQ=255)                                                                                                                  aCGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGc               <  2:994996/140‑1 (MQ=255)                                                                                                                   cGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCg              <  2:256231/140‑1 (MQ=255)                                                                                                                   cGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCg              <  2:1002611/140‑1 (MQ=255)                                                                                                                   cGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCg              >  2:891989/1‑140 (MQ=255)                                                                                                                   cGCCGTATCAATTTCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCg              <  1:1070859/140‑1 (MQ=255)                                                                                                                             aTTTCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGAt    >  2:118926/1‑140 (MQ=255)                                                                                                                               ttCACCTGCCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTa  <  1:118926/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                    GGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGTCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTA  >  NC_000913/2080055‑2080320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑