Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,082,980	G→A	R75R (CGG→CGA)	sbcB →	exodeoxyribonuclease I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,082,980	0	G	A	100.0%	131.9 / NA	38	R75R (CGG→CGA)	sbcB	exodeoxyribonuclease I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/14);  total (24/14)

CGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTT  >  NC_000913/2082848‑2083114                                                                                                                                     |                                                                                                                                       cGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAg                                                                                                                                 >  2:80249/1‑140 (MQ=255)                  aTAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCcgcg                                                                                                                >  1:857151/1‑140 (MQ=255)                       gAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTCTgc                                                                                                           <  1:467664/140‑1 (MQ=255)                        aaTTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTgcc                                                                                                          >  2:74583/1‑140 (MQ=255)                          ttCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTgccgc                                                                                                        <  1:896400/140‑1 (MQ=255)                                gTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTAt                                                                                                  >  1:385077/1‑140 (MQ=255)                                   aTCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCa                                                                                               >  1:135055/1‑140 (MQ=255)                                   aTCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCa                                                                                               >  1:867359/1‑140 (MQ=255)                                   aTCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCACCCGTATTCa                                                                                               >  2:813395/1‑140 (MQ=255)                                           aCCCGAAGTCTTTTACTGCAAGCCTGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCttt                                                                                       >  2:913306/1‑140 (MQ=255)                                           aCCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCttt                                                                                       >  2:57938/1‑140 (MQ=255)                                            cccGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCtttt                                                                                      <  1:80249/140‑1 (MQ=255)                                                aaGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCACCCGTATTCACTCGCTTTTtacc                                                                                  <  1:813395/140‑1 (MQ=255)                                                        tACTGCAAGCCTGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGaa                                                                          <  1:913306/140‑1 (MQ=255)                                                             cAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCt                                                                     >  2:970671/1‑140 (MQ=255)                                                                   cGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTc                                                               <  1:495798/140‑1 (MQ=255)                                                                          gaCTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTa                                                        >  1:1022570/1‑140 (MQ=255)                                                                                       agccagGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGt                                           >  1:103184/1‑140 (MQ=255)                                                                                       agccagGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGt                                           >  2:589057/1‑140 (MQ=255)                                                                                       agccagGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGt                                           >  2:448142/1‑140 (MQ=255)                                                                                         ccagGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGttt                                         >  1:56287/1‑140 (MQ=255)                                                                                               gCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgacga                                   <  1:589057/140‑1 (MQ=255)                                                                                               gCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgacga                                   <  1:448142/140‑1 (MQ=255)                                                                                                   tATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgacgacg                                 >  1:356300/1‑138 (MQ=255)                                                                                                   tATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgacgacg                                 <  2:356300/138‑1 (MQ=255)                                                                                                            ccGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACg                       >  2:532258/1‑139 (MQ=255)                                                                                                            ccGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACg                       <  1:532258/139‑1 (MQ=255)                                                                                                             cGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgacga                                   <  2:1032470/126‑1 (MQ=255)                                                                                                             cGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgacga                                   <  2:112720/126‑1 (MQ=255)                                                                                                             cGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgacga                                   >  1:112720/1‑126 (MQ=255)                                                                                                             cGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgacga                                   >  1:1032470/1‑126 (MQ=255)                                                                                                                  ttACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACAtt                >  1:742395/1‑140 (MQ=255)                                                                                                                          cAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCg        >  1:370709/1‑140 (MQ=255)                                                                                                                          cAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCg        >  1:134382/1‑140 (MQ=255)                                                                                                                          cAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCg        >  1:1067215/1‑140 (MQ=255)                                                                                                                           aGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCCGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGt       <  2:135055/140‑1 (MQ=255)                                                                                                                              aaGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAAt    <  2:385077/140‑1 (MQ=255)                                                                                                                               aGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAAtt   >  1:19555/1‑140 (MQ=255)                                                                                                                                gCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAAttt  >  2:476398/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                       CGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTT  >  NC_000913/2082848‑2083114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑