Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,117,718	G→A	D229D (GAC→GAT)	wcaK ←	putative colanic acid biosynthesis pyruvyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,117,718	0	G	A	100.0%	276.7 / NA	77	D229D (GAC→GAT)	wcaK	putative colanic acid biosynthesis pyruvyl transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (42/35);  total (42/35)

CTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACGTCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATT  >  NC_000913/2117588‑2117854                                                                                                                                   |                                                                                                                                         cTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATccagccag                                                                                                                                 >  1:484804/1‑140 (MQ=255) cTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATccagccag                                                                                                                                 >  2:209321/1‑140 (MQ=255)   tCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAgtg                                                                                                                               >  2:656033/1‑140 (MQ=255)    cATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAgtgt                                                                                                                              >  1:663069/1‑140 (MQ=255)     aTCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGtt                                                                                                                             >  2:191429/1‑140 (MQ=255)      tCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTg                                                                                                                            <  1:1029096/140‑1 (MQ=255)       cGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGa                                                                                                                           >  2:392402/1‑140 (MQ=255)        gagaATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGaa                                                                                                                          <  1:209321/140‑1 (MQ=255)        gagaATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGaa                                                                                                                          >  2:98443/1‑140 (MQ=255)         agaATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAAc                                                                                                                         >  1:577482/1‑140 (MQ=255)            aTGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGc                                                                                                                      <  2:224007/140‑1 (MQ=255)             tGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCa                                                                                                                     >  2:695849/1‑140 (MQ=255)             tGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCa                                                                                                                     <  1:695849/140‑1 (MQ=255)                     accaccCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGc                                                                                                             >  2:135743/1‑140 (MQ=255)                         ccccGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTg                                                                                                         >  1:730649/1‑140 (MQ=255)                            cGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAg                                                                                                      >  2:874532/1‑140 (MQ=255)                             ggCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGGAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGt                                                                                                     <  1:10776/140‑1 (MQ=255)                             ggCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGt                                                                                                     <  2:854713/140‑1 (MQ=255)                             ggCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGt                                                                                                     <  1:98443/140‑1 (MQ=255)                               cAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCg                                                                                                            >  1:990803/1‑131 (MQ=255)                               cAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGtct                                                                                                   >  1:170835/1‑140 (MQ=255)                               cAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGCCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCg                                                                                                            <  2:990803/131‑1 (MQ=255)                                aaaGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGtctt                                                                                                  <  1:886438/140‑1 (MQ=255)                                aaaGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGtctt                                                                                                  <  1:656033/140‑1 (MQ=255)                                aaaGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGtctt                                                                                                  <  2:730649/140‑1 (MQ=255)                                 aaGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGtcttc                                                                                                 <  2:577482/140‑1 (MQ=255)                                  aGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGtcttct                                                                                                <  2:484804/140‑1 (MQ=255)                                    gCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCtgt                                                                                              >  1:372425/1‑140 (MQ=255)                                    gCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCtgt                                                                                              >  1:1022213/1‑140 (MQ=255)                                    gCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCtgt                                                                                              >  2:639231/1‑140 (MQ=255)                                        tttCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTgtgg                                                                                          >  2:884949/1‑140 (MQ=255)                                         ttCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTgtggt                                                                                         <  1:135743/140‑1 (MQ=255)                                              aCGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGTCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCg                                                                                    >  1:1085202/1‑140 (MQ=255)                                                   tgttgAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAg                                                                               >  1:248536/1‑140 (MQ=255)                                                    gttgAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGc                                                                              >  1:908100/1‑140 (MQ=255)                                                    gttgAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGc                                                                              >  2:1125186/1‑140 (MQ=255)                                                          tggtgCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCAcgcg                                                                        >  2:377556/1‑140 (MQ=255)                                                          tggtgCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCAcgcg                                                                        <  2:1022213/140‑1 (MQ=255)                                                            gtgCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCTGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGt                                                                      >  2:975374/1‑140 (MQ=255)                                                            gtgCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGCACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATAGACGAGCCACGCggg                                                                      >  2:827063/1‑139 (MQ=255)                                                            gtgCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGt                                                                      >  2:723919/1‑140 (MQ=255)                                                               ccGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATc                                                                   >  2:193085/1‑140 (MQ=255)                                                               ccGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATc                                                                   >  1:11886/1‑140 (MQ=255)                                                               ccGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGGGCGGCAACATCCCGCCAGTGTTGAACTGCATAGCTGGCGGTGAAGTCTTCTGTGTGGAGATGGATCAGCCACGCGGCATc                                                                   >  2:168788/1‑140 (MQ=255)                                                                    aCGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGc                                                              <  1:884949/140‑1 (MQ=255)                                                                       tttGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCAt                                                           >  2:398868/1‑140 (MQ=255)                                                                       tttGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCAt                                                           >  2:395285/1‑140 (MQ=255)                                                                       tttGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCAt                                                           >  2:689322/1‑140 (MQ=255)                                                                           tCAAACGGTGCCAGTTCGCGCAGGGTAATGGTCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTc                                                       <  2:1085202/140‑1 (MQ=255)                                                                           tCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTc                                                       <  2:170835/140‑1 (MQ=255)                                                                           tCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTc                                                       <  1:689322/140‑1 (MQ=255)                                                                                   tGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTg                                               >  1:590327/1‑140 (MQ=255)                                                                                   tGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTg                                               <  2:372425/140‑1 (MQ=255)                                                                                   tGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTg                                               <  1:1125186/140‑1 (MQ=255)                                                                                   tGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTg                                               <  1:91935/140‑1 (MQ=255)                                                                                    gCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGc                                              <  1:398868/140‑1 (MQ=255)                                                                                    gCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGc                                              <  2:11886/140‑1 (MQ=255)                                                                                       aGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCggt                                           <  1:639231/140‑1 (MQ=255)                                                                                         ttCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCggtgg                                         <  1:605015/140‑1 (MQ=255)                                                                                             cgcAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGat                                     >  2:826848/1‑140 (MQ=255)                                                                                                  ggTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATtgc                                <  1:17598/140‑1 (MQ=255)                                                                                                            aCCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCg                            >  2:304811/1‑134 (MQ=255)                                                                                                            aCCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCg                            <  1:304811/134‑1 (MQ=255)                                                                                                            aCCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTtcat                      >  1:1057385/1‑140 (MQ=255)                                                                                                            aCCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTtcat                      >  1:288310/1‑140 (MQ=255)                                                                                                            aCCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTtcat                      <  1:651452/140‑1 (MQ=255)                                                                                                            aCCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTtcat                      >  1:633592/1‑140 (MQ=255)                                                                                                            aCCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTtcat                      <  1:168788/140‑1 (MQ=255)                                                                                                              cGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTtcatca                    >  2:487177/1‑140 (MQ=255)                                                                                                              cGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTtcatca                    >  1:869389/1‑140 (MQ=255)                                                                                                                     tgttgtGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCaaa             <  1:395285/140‑1 (MQ=255)                                                                                                                     tgttgtGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCaaa             <  1:191429/140‑1 (MQ=255)                                                                                                                            cGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGAcc      >  2:1076574/1‑140 (MQ=255)                                                                                                                            cGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGAcc      >  1:979543/1‑140 (MQ=255)                                                                                                                            cGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGAcc      >  1:974203/1‑140 (MQ=255)                                                                                                                               cAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGAt   <  2:633592/140‑1 (MQ=255)                                                                                                                               cAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGAt   <  2:590327/140‑1 (MQ=255)                                                                                                                                aaCATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGAtt  <  1:193085/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                         CTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACGTCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATT  >  NC_000913/2117588‑2117854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑