Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,196,727	T→C	V85A (GTA→GCA)	yehF →	putative protein YehF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,196,727	0	T	C	100.0%	167.4 / NA	45	V85A (GTA→GCA)	yehF	putative protein YehF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/20);  total (25/20)

AAAGTTTTTCAGATGCTGCGGCAGCGGCAAAAGCGGAGCTTAAGCTGATTGCGGAGAAGGTGAAGAAGGGGTATGTGGAGCAAGCGAAGGATAATTCTTTGCAACCTTCCCAAACGGTAACGGGCTCTCTCAAGGTAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAACCCGTGCGCCTGACAAAAATACAGATGCTGTTTTACCGTGGCTGGCGAAAGATATTGCTGTCGTTTTTCCGCCCGAAGTTGTA  >  NC_000913/2196592‑2196860                                                                                                                                        |                                                                                                                                      aaaGTTTTTCAGATGCTGCGGCAGCGGCAAAAGCGGAGCTTAAGCTGATTGCGGAGAAGGTGAAGAAGGGGTATGTGGAGCAAGCGAAGGATAATTCTTTGCAACCTTCCCAAACGGTAACGGGCTCTCTCAAGGCAGCg                                                                                                                                   <  2:82216/140‑1 (MQ=255)    gTTTTTCAGATGCTGCGGCAGCGGCAAAAGCGGAGCTTAAGCTGATTGCGGAGAAGGTGAAGAAGGGGTATGTGGAGCAAGCGAAGGATAATTCTTTGCAACCTTCCCAAACGGTAACGGGCTCTCTCAAGGCAGCGGAc                                                                                                                                <  2:929793/140‑1 (MQ=255)            gATGCTGCGGCAGCGGCAAAAGCGGAGCTTAAGCTGATTGCGGAGAAGGTGAAGAAGGGGTATGTGGAGCAAGCGAAGGATAATTCTTTGCAACCTTCCCAAACGGTAACGGGCTCTCTCAAGGCAGCGGACTTATccac                                                                                                                        <  2:1025225/140‑1 (MQ=255)              tgctgcGGCAGCGGCAAAAGCGGAGCTTAAGCTGATTGCGGAGAAGGTGAAGAAGGGGTATGTGGAGCAAGCGAAGGATAATTCTTTGCAACCTTCCCAAACGGTAACGGGCTCTCTCAAGGCAGCGGACTTATccacca                                                                                                                      <  1:818239/140‑1 (MQ=255)                   cggcagcggcaAAAGCGGAGCTTAAGCTGATTGCGGAGAAGGTGAAGAAGGGGTATGTGGAGCAAGCGAAGGATAATTCTTTGCAACCTTCCCAAACGGTAACGGGCTCTCTCAAGGCAGCGGACTTATCCACCattatt                                                                                                                 >  1:55575/1‑140 (MQ=255)                         cggcaAAAGCGGAGCTTAAGCTGATTGCGGAGAAGGTGAAGAAGGGGTATGTGGAGCAAGCGAAGGATAATTCTTTGCAACCTTCCCAAACGGTAACGGGCTCTCTCAAGGCAGCGGACTTATCCACCATTATTCAGGaa                                                                                                           >  1:512163/1‑140 (MQ=255)                            caAAAGCGGAGCTTAAGCTGATTGCGGAGAAGGTGAAGAAGGGGTATGTGGAGCAAGCGAAGGATAATTCTTTGCAACCTTCCCAAACGGTAACGGGCTCTCTCAAGGCAGCGGACTTATCCACCATTATTCAGGaacaa                                                                                                        <  1:431337/140‑1 (MQ=255)                                          aaGCTGATTGCGGAGAAGGTGAAGAAGGGGTATGTGGAGCAAGCGAAGGATAATTCTTTGCAACCTTCCCAAACGGTAACGGGCTCTCTCAAGGCAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGc                                                                                          <  2:512163/140‑1 (MQ=255)                                                 ttGCGGAGAAGGTGAAGAAGGGGTATGTGGAGCAAGCGAAGGATAATTCTTTGCAACCTTCCCAAACGGTAACGGGCTCTCTCAAGGCAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAAcc                                                                                   >  1:2406/1‑140 (MQ=255)                                                 ttGCGGAGAAGGTGAAGAAGGGGTATGTGGAGCAAGCGAAGGATAATTCTTTGCAACCTTCCCAAACGGTAACGGGCTCTCTCAAGGCAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAAcc                                                                                   >  2:934010/1‑140 (MQ=255)                                                 ttGCGGAGAAGGTGAAGAAGGGGTATGTGGAGCAAGCGAAGGATAATTCTTTGCAACCTTCCCAAACGGTAACGGGCTCTCTCAAGGCAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAAcc                                                                                   >  2:260812/1‑140 (MQ=255)                                                 ttGCGGAGAAGGTGAAGAAGGGGTATGTGGAGCAAGCGAAGGATAATTCTTTGCAACCTTCCCAAACGGT‑ACGGGCTCTCTCAAGGCAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAAccc                                                                                  >  2:919739/1‑140 (MQ=255)                                                     ggAGAAGGTGAAGAAGGGGTATGTGGAGCAAGCGAAGGATAATTCTTTGCAACCTTCCCAAACGGTAACGGGCTCTCTCAAGGCAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAACCCGTg                                                                               <  2:718871/140‑1 (MQ=255)                                                          aGGTGAAGAAGGGGTATGTGGAGCAAGCGAAGGATAATTCTTTGCAACCTTCCCAAACGGTAACGGGCTCTCTCGAGGCAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAACCCGTGCGCCt                                                                          >  2:407090/1‑140 (MQ=255)                                                            gTGAAGAAGGGGTATGTGGAGCAAGCGAAGGATAATTCTTTGCAACCTTCCCAAACGGTAACGGGCTCTCTCAAGGCAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAACCCGTGCGCCTGa                                                                        <  2:140134/140‑1 (MQ=255)                                                                   agGGGTATGTGGAGCAAGCGAAGGATAATTCTTTGCAACCTTCCCAAACGGTAACGGGCTCTCTCAAGGCAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAACCCGTGCGCCTGACAAAAAt                                                                 >  1:890051/1‑140 (MQ=255)                                                                   agGGGTATGTGGAGCAAGCGAAGGATAATTCTTTGCAACCTTCCCAAACGGTAACGGGCTCTCTCAAGGCAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAACCCGTGCGCCTGACAAAAAt                                                                 <  1:934010/140‑1 (MQ=255)                                                                     gggTATGTGGAGCAAGCGAAGGATAATTCTTTGCAACCTTCCCAAACGGTAACGGGCTCTCTCAAGGCAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAACCCGTGCGCCt                                                                          >  1:950728/1‑129 (MQ=255)                                                                     gggTATGTGGAGCAAGCGAAGGATAATTCTTTGCAACCTTCCCAAACGGTAACGGGCTCTCTCAAGGCAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAACCCGTGCGCCt                                                                          <  2:950728/129‑1 (MQ=255)                                                                     gggTATGTGGAGCAAGCGAAGGATAATTCTTTGCAACCTTCCCAAACGGTAACGGGCTCTCTCAAGGCAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAACCCGTGCGCCTGACAAAAATAc                                                               >  1:759656/1‑140 (MQ=255)                                                                     gggTATGTGGAGCAAGCGAAGGATAATTCTTTGCAACCTTCCCAAACGGTAACGGGCTCTCTCAAGGCAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAACCCGTGCGCCTGACAAAAATAc                                                               >  1:975595/1‑140 (MQ=255)                                                                      ggTATGTGGAGCAAGCGAAGGATAATTCTTTGCAACCTTCCCAAACGGTAACGGGCTCTCTCAAGGCAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAACCCGTGCGCCTGAc                                                                       <  1:58162/131‑1 (MQ=255)                                                                      ggTATGTGGAGCAAGCGAAGGATAATTCTTTGCAACCTTCCCAAACGGTAACGGGCTCTCTCAAGGCAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAACCCGTGCGCCTGAc                                                                       >  2:58162/1‑131 (MQ=255)                                                                        tATGTGGAGCAAGCGAAGGATAATTCTTTGCAACCTTCCCAAACGGTAACGGGCTCTCTCGAGGCAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAACCCGTGCGCCTGACAAAAATACAGa                                                            <  1:407090/140‑1 (MQ=255)                                                                                   aGCGAAGGATAATTCTTTGCAACCTTCCCAAACGGTAAAGGGTTCTCTCAAGGCAGCGTACTTATGGACCATTATTAAGGAACAATCTTCTTTAGTAGCAGAAACGCGTGCGCCTGACAAAAATACAGATGCTGTTTTAc                                                 >  2:1069735/1‑140 (MQ=255)                                                                                    gCGAAGGATAATTCTTTGCAACCTTCCCAAACGGTAACGGGCTCTCTCAAGGCAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAACCCGTGCGCCTGACAAAAATACAGATGCTGTTTTAcc                                                >  1:531969/1‑140 (MQ=255)                                                                                    gCGAAGGATAATTCTTTGCAACCTTCCCAAACGGTAACGGGCTCTCTCAAGGCAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAACCCGTGCGCCTGACAAAAATACAGATGCTGTTTTAcc                                                >  1:421887/1‑140 (MQ=255)                                                                                        aGGATAATTCTTTGCAACCTTCCCAAACGGT‑ACGGGCTCTCTCAAGGCAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAACCCGTGCGCCTGACAAAAATACAGATGCTGTTTTACCGtggc                                           <  1:919739/140‑1 (MQ=255)                                                                                              aTTCTTTGCAACCTTCCCAAACGGTAACGGGCTCTCTCAAGGCAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAACCCGTGCGCCTGACAAAAATACAGATGCTGTTTTACCGTGGCTGGCg                                      <  2:719657/140‑1 (MQ=255)                                                                                                    tGCAACCTTCCCAAACGGTAACGGGCTCTCTCAAGGCAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAACCCGTGCGCCTGACAAAAATACAGATGCTGTTTTACCGTGGCTGGCGAAAGat                                >  2:463282/1‑140 (MQ=255)                                                                                                    tGCAACCTTCCCAAACGGTAACGGGCTCTCTCAAGGCAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAACCCGTGCGCCTGACAAAAATACAGATGCTGTTTTACCGTGGCTGGCGAAAGat                                >  2:593545/1‑140 (MQ=255)                                                                                                    tGCAACCTTCCCAAACGGTAACGGGCTCTCTCAAGGCAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAACCCGTGCGCCTGACAAAAATACAGATGCTGTTTTACCGTGGCTGGCGAAAGat                                >  2:727730/1‑140 (MQ=255)                                                                                                          cTTCCCAAACGGTAACGGGCTCTCTCAAGGCAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAACCCGTGCGCCTGACAAAAATACAGATGCTGTTTTACCGTGGCTGGCGAAAGATATTGCt                          <  1:463282/140‑1 (MQ=255)                                                                                                            tCCCAAACGGTAACGGGCTCTCTCAAGGCAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAACCCGTGCGCCTGACAAAAATACAGATGCTGTTTTACCGTGGCTGGCGAAAGATATTGCTGt                        >  1:438202/1‑140 (MQ=255)                                                                                                                   cGGTAACGGGCTCTCTCAAGGCAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAACCCGTGCGCCTGACAAAAATACAGATGCTGTTTTACCGTGGCTGGCGAAAGATATTGCTGTCGttttt                 <  2:975595/140‑1 (MQ=255)                                                                                                                      tAACGGGCTCTCTCAAGGCAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAACCCGTGCGCCTGACAAAAATACAGATGCTGTTTTACCGTGGCTGGCGAAAGATATTGCTGTCGTTTTTCCg              >  1:398308/1‑140 (MQ=255)                                                                                                                          gggCTCTCTCAAGGCAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAACCCGTGCGCCTGACAAAAATACAGATGCTGTTTTACCGTGGCTGGCGAAAGATATTGCTGTCGTTTTTCCGCCCg          <  1:153822/140‑1 (MQ=255)                                                                                                                          gggCTCTCTCAAGGCAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAACCCGTGCGCCTGACAAAAATACAGATGCTGTTTTACCGTGGCTGGCGAAAGATATTGCTGTCGTTTTTCCGCCCg          <  1:727730/140‑1 (MQ=255)                                                                                                                            gCTCTCTCAAGGCAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAACCCGTGCGCCTGACAAAAATACAGATGCTGTTTTACCGTGGCTGGCGAAAGATATTGCTGTCGTTTTTCCGCCCGaa        <  2:531969/140‑1 (MQ=255)                                                                                                                              tctctcAAGGCAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAACCCGTGCGCCTGACAAAAATACAGATGCTGTTTTACCGTGGCTGGCGAAAGATATTGCTGTCGTTTTTCCGCCCGAAGt      >  1:490384/1‑140 (MQ=255)                                                                                                                               ctctcAAGGCAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAACCCGTGCGCCTGACAAAAATACAGATGCTGTTTTACCGTGGCTGGCGAAAGATATTGCTGTCGTTTTTCCGCCCGAAGtt     >  1:845897/1‑140 (MQ=255)                                                                                                                                tctcAAGGCAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAACCCGTGCGCCTGACAAAAATACAGATGCTGTTTTACCGTGGCTGGCGAAAGATATTGCTGTCGTTTTTCCGCCCGAAGTTg    >  1:505235/1‑140 (MQ=255)                                                                                                                                tctcAAGGCAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAACCCGTGCGCCTGACAAAAATACAGATGCTGTTTTACCGTGGCTGGCGAAAGATATTGCTGTCGTTTTTCCGCCCGAAGTTg    >  2:889868/1‑140 (MQ=255)                                                                                                                                tctcAAGGCAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAACCCGTGCGCCTGACAAAAATACAGATGCTGTTTTACCGTGGCTGGCGAAAGATATTGCTGTCGTTTTTCCGCCCGAAGTTg    >  2:712971/1‑140 (MQ=255)                                                                                                                                  tcAAGGCAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAACCCGTGCGCCTGACAAAAATACAGATGCTGTTTTACCGTGGCTGGCGAAAGATATTGCTGTCGTTTTTCCGCCCGAAGTTGTa  <  2:398308/140‑1 (MQ=255)                                                                                                                                  tcAAGGCAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAACCCGCGCGCCTGACAAAAATACAGATGCTGTTTTACCGTGGCTGGCGAAAGATATTGCTGTCGTTTTTCCGCCCGAAGTTGTa  >  2:397883/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      AAAGTTTTTCAGATGCTGCGGCAGCGGCAAAAGCGGAGCTTAAGCTGATTGCGGAGAAGGTGAAGAAGGGGTATGTGGAGCAAGCGAAGGATAATTCTTTGCAACCTTCCCAAACGGTAACGGGCTCTCTCAAGGTAGCGGACTTATCCACCATTATTCAGGAACAACCCTCTTTCGTAGCAGAAACCCGTGCGCCTGACAAAAATACAGATGCTGTTTTACCGTGGCTGGCGAAAGATATTGCTGTCGTTTTTCCGCCCGAAGTTGTA  >  NC_000913/2196592‑2196860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑