Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,211,471	G→A	A83T (GCC→ACC)	yehR →	DUF1307 domain‑containing lipoprotein YehR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,211,471	0	G	A	100.0%	171.7 / NA	50	A83T (GCC→ACC)	yehR	DUF1307 domain‑containing lipoprotein YehR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/21);  total (29/21)

CGGCACTGAAATTGCCATTACCTATGTCTACAAAGGTGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCGCCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATGGAAAAAGTGGATTTTAAAGCCCTGCAGGGTATTTCAGGAATCAACGTTTC  >  NC_000913/2211335‑2211604                                                                                                                                         |                                                                                                                                      cGGCACTGAAATTGCCATTACCTATGTCTACAAAGGTGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCa                                                                                                                                    <  2:132464/140‑1 (MQ=255)      cTGAAATTGCCATTACCTATGTCTACAAAGGTGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATAc                                                                                                                               >  2:474212/1‑140 (MQ=255)              gCCATTACCTATGTCTACAAAGGTGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACAt                                                                                                                       >  1:180923/1‑140 (MQ=255)              gCCATTACCTATGTCTACAAAGGTGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACAt                                                                                                                       >  2:199212/1‑140 (MQ=255)              gCCATTACCTATGTCTACAAAGGTGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACAt                                                                                                                       >  1:686854/1‑140 (MQ=255)                cATTACCTATGTCTACAAAGGTGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATcg                                                                                                                     >  2:316279/1‑140 (MQ=255)                   tACCTATGTCTACAAAGGTGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATcgcag                                                                                                                  >  2:672357/1‑138 (MQ=255)                            cTACAAAGGTGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTgaag                                                                                                         <  2:251621/140‑1 (MQ=255)                             tACAAAGGTGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTgaaga                                                                                                        >  1:423254/1‑140 (MQ=255)                               cAAAGGTGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATTGCGGGTGTTGAAGaaa                                                                                                      >  1:304768/1‑140 (MQ=255)                               cAAAGGTGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGaaa                                                                                                      >  1:834078/1‑140 (MQ=255)                                 aaGGTGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGaaaaa                                                                                                    <  2:834078/140‑1 (MQ=255)                                 aaGGTGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGaaaaa                                                                                                    <  1:316279/140‑1 (MQ=255)                                  aGGTGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAAt                                                                                                   <  2:631270/140‑1 (MQ=255)                                  aGGTGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAAt                                                                                                   <  1:551596/140‑1 (MQ=255)                                     tGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTaa                                                                                                >  2:56325/1‑140 (MQ=255)                                     tGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTaa                                                                                                >  2:540952/1‑140 (MQ=255)                                     tGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTaa                                                                                                >  1:233874/1‑140 (MQ=255)                                      gACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAAc                                                                                               <  2:686854/140‑1 (MQ=255)                                        cAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCt                                                                                             <  1:178855/140‑1 (MQ=255)                                        cAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCt                                                                                             <  2:857069/140‑1 (MQ=255)                                        cAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCt                                                                                             <  1:199212/140‑1 (MQ=255)                                            gTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATAc                                                                                         <  1:791257/140‑1 (MQ=255)                                              gCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCg                                                                                       >  1:405550/1‑140 (MQ=255)                                              gCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCg                                                                                       >  2:288727/1‑140 (MQ=255)                                                             tGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAgg                                                                        <  2:1113512/140‑1 (MQ=255)                                                                    aaaaTTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGt                                                                 >  2:1067414/1‑140 (MQ=255)                                                                    aaaaTTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGt                                                                 >  1:701344/1‑140 (MQ=255)                                                                     aaaTTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTg                                                                <  2:423254/140‑1 (MQ=255)                                                                      aaTTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTAcg                                                                              <  1:941836/125‑1 (MQ=255)                                                                      aaTTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTAcg                                                                              >  2:941836/1‑125 (MQ=255)                                                                                  cTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATgg                                                   >  1:817591/1‑140 (MQ=255)                                                                                  cTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATgg                                                   <  2:318949/140‑1 (MQ=255)                                                                                           tGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATGGAAAAAGTgg                                          >  2:766583/1‑140 (MQ=255)                                                                                                         aagaTGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATGGAAAAAGTGGATTTTAAAGCCCTg                            <  1:346948/140‑1 (MQ=255)                                                                                                          agaTGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATGGAAAAAGTGGATTTTAAAGCCCTGc                           >  2:593598/1‑140 (MQ=255)                                                                                                           gaTGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATGGAAAAAGTGGATTTTAAAGCCCTGCa                          <  1:537627/140‑1 (MQ=255)                                                                                                              gctgcCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATGGAAAAAGTGGATTTTAAAGCCCTGCAggg                       <  1:288727/140‑1 (MQ=255)                                                                                                                  ccAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATGGAAAAAGTGGATTTTAAAGCCCTg                            >  2:358646/1‑131 (MQ=255)                                                                                                                  ccAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATGGAAAAAGTGGATTTTAAAGCCCTg                            <  1:358646/131‑1 (MQ=255)                                                                                                                   cAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATGGAAAAAGTGGATTTTAAAGCCCTGCAGGGTAttt                  >  1:761738/1‑140 (MQ=255)                                                                                                                     aGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATGGAAAAAGTGGATTTTAAAGCCCTGCAGGGTATTTCa                >  1:547172/1‑140 (MQ=255)                                                                                                                      gACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATGGAAAAAGTGGATTTTAAAGCCCTGCAGGGTATTTCAg               >  2:363404/1‑140 (MQ=255)                                                                                                                          cTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATGGAAAAAGTGGATTTTAAAGCCCTGCAg                         <  1:1040304/126‑1 (MQ=255)                                                                                                                          cTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATGGAAAAAGTGGATTTTAAAGCCCTGCAg                         >  2:1040304/1‑126 (MQ=255)                                                                                                                           ttGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATGGAAAAAGTGGATTTTAAAGCCCTGCAGGGTATTTCAGGAATc          <  2:180923/140‑1 (MQ=255)                                                                                                                            tGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATGGAAAAAGTGGATTTTAAAGCCCTGCAGGGTATTTCAGGAATCa         >  1:386506/1‑140 (MQ=255)                                                                                                                                ccGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATGGAAAAAGTGGATTTTAAAGCCCTGCAGGGTATTTCAGGAATCAACGt     >  2:981357/1‑140 (MQ=255)                                                                                                                                 cGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATGGAAAAAGTGGATTTTAAAGCCCTGCAGGGTATTTCAGGAATCAACGtt    >  1:931705/1‑140 (MQ=255)                                                                                                                                   ttAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATGGAAAAAGTGGATTTTAAAGCCCTGCAGGGTATTTCAGGAATCAACGTTTc  >  2:1040631/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      CGGCACTGAAATTGCCATTACCTATGTCTACAAAGGTGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCGCCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATGGAAAAAGTGGATTTTAAAGCCCTGCAGGGTATTTCAGGAATCAACGTTTC  >  NC_000913/2211335‑2211604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑