Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,262,984	T→C	N171S (AAC→AGC)	fruB ←	fructose‑specific PTS multiphosphoryl transfer protein FruB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,262,984	0	T	C	100.0%	178.5 / NA	48	N171S (AAC→AGC)	fruB	fructose‑specific PTS multiphosphoryl transfer protein FruB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/19);  total (29/19)

AATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCGGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAG  >  NC_000913/2262851‑2263120                                                                                                                                      |                                                                                                                                         aaTCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCGGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGCTCAgcg                                                                                                                                    >  1:307260/1‑140 (MQ=255)   tCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCGGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGCTCAGCGcc                                                                                                                                  >  1:696784/1‑140 (MQ=255)      cgcTACGCAGTTTGCCTTCGGCGCTACCGCTCAGCCAGATCCCCTGTCCGAGATTCAGCGTTTGTTCATTGATGGCTTTGGGGACAAAAGTGGCGTCACCTCCCCCCGCCTCTTTCAGACGCGCAGCGCTCAGCGCCTGa                                                                                                                               <  2:696784/140‑1 (MQ=255)                     cTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCGGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCg                                                                                                                >  1:897997/1‑140 (MQ=255)                               aTCGCTCAGCCAGATCCCCTGTCCGAGATTCAGCGTTTGTTCATTGATGGTTTTGGTGACGAAGGTGGCGTCACCCCCCCCCGCCTCTTTCAGACGCGCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGcgacga                                                                                                      <  2:616890/140‑1 (MQ=255)                                          aGATACCCTGTCCGAGATTCAGCGGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTc                                                                                           >  1:616890/1‑140 (MQ=255)                                                  tGTCCGAGATTCAGCGGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTc                                                                                   >  2:450205/1‑140 (MQ=255)                                                  tGTCCGAGATTCAGCGGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTc                                                                                   >  1:926900/1‑140 (MQ=255)                                                    tCCGAGATTCAGCGGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGt                                                                                 >  1:627584/1‑140 (MQ=255)                                                    cccGAGTTTCGGGGGTTGTTCATTGATGGTTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGt                                                                                 <  2:897997/139‑1 (MQ=255)                                                      cGAGATTCAGCGGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTg                                                                               >  1:59639/1‑140 (MQ=255)                                                       gagtTTGAGGGGTTTTTTTTTGATGGTTTGGGTGACAAAGGTGGCGTCAACGCCCCCCGCCTCTTTCAGACGCGCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGt                                                                              <  1:1125180/140‑1 (MQ=255)                                                       gagaTTCAGCGGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGt                                                                              <  1:937954/140‑1 (MQ=255)                                                         gaTTCAGCGGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCg                                                                            >  1:358064/1‑140 (MQ=255)                                                          aTTCAGCGGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGa                                                                           >  2:761811/1‑140 (MQ=255)                                                              aGCGGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCtt                                                                       >  2:384887/1‑140 (MQ=255)                                                              aGCGGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCtt                                                                       >  1:773026/1‑140 (MQ=255)                                                                cGGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCa                                                                     <  1:450205/140‑1 (MQ=255)                                                                   ttgttCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAgct                                                                  >  1:784673/1‑140 (MQ=255)                                                                    tgttCATTGATGGTTTTGGTGACGAAAGGGGCGTCACCTGCCCCCGCCTCTTTCAGACGCGCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAgctg                                                                 <  1:323773/140‑1 (MQ=255)                                                                      ttatttGAGGCCTTTGGTGAAAAAAGGGGCCCCATCCGCCTCCGCCTCTTTCAGACGCGCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAgctgct                                                               <  2:16848/136‑1 (MQ=255)                                                                              tGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGc                                                       >  1:448515/1‑140 (MQ=255)                                                                                gCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGGGCTTCAGCTGCTCACTCTGCtt                                                     >  1:804705/1‑140 (MQ=255)                                                                                gCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCtt                                                     >  1:586769/1‑140 (MQ=255)                                                                                gCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCtt                                                     >  1:16848/1‑140 (MQ=255)                                                                                gCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCtt                                                     >  2:445974/1‑140 (MQ=255)                                                                                gCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCtt                                                     >  1:1042716/1‑140 (MQ=255)                                                                                  tttGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCtttt                                                   >  1:575543/1‑140 (MQ=255)                                                                                  ttgggTGACGAAGGTGGCGTCACCTGCCCCCGCCTCTTTCAGACGCGCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCtttt                                                   <  2:627584/137‑1 (MQ=255)                                                                                    gggTGACGAAAGTGGGGACACCTGCCCCCGCCTCTTTCAGACGCGCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCg                                                 <  1:448463/139‑1 (MQ=255)                                                                                           cgaaGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCtttt                                                   >  1:828878/3‑131 (MQ=255)                                                                                           cgaaGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCtttt                                                   <  2:828878/129‑1 (MQ=255)                                                                                            aaaGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAg                                         >  2:119078/1‑140 (MQ=255)                                                                                               gTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTaa                                      <  1:119078/140‑1 (MQ=255)                                                                                                   cGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATgcg                                  >  2:534030/1‑140 (MQ=255)                                                                                                     ccaccTGCCCCCGCCTCTTTCAGACGCGCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGcgcg                                <  1:589487/136‑1 (MQ=255)                                                                                                      caccgccccccGCCTCTTTCAGACGCGCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGa                               <  2:804705/134‑1 (MQ=255)                                                                                                            cccccGCCTCTTTCAGACGCGCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGttct                         <  1:384887/140‑1 (MQ=255)                                                                                                            cccccGCCTCTTTCAGACGCGCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGttct                         >  1:830363/1‑140 (MQ=255)                                                                                                                cGCCTCTTTCAGACGCGCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTctg                     <  1:445974/140‑1 (MQ=255)                                                                                                                           gACGCGCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCt          <  1:534030/140‑1 (MQ=255)                                                                                                                             cgcgCAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGa        >  2:613375/1‑140 (MQ=255)                                                                                                                              gcgcAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGAc       <  2:830363/140‑1 (MQ=255)                                                                                                                               cgcAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGAc       <  2:369235/139‑1 (MQ=255)                                                                                                                               cgcAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGAc       >  1:369235/1‑139 (MQ=255)                                                                                                                                gcAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACtt     >  2:64177/1‑140 (MQ=255)                                                                                                                                gcAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACtt     >  1:806141/1‑140 (MQ=255)                                                                                                                                 cAGCGCTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTc    >  1:16760/1‑140 (MQ=255)                                                                                                                                   gcgcTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAg  <  1:487428/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         AATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCGGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAG  >  NC_000913/2262851‑2263120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑