Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,276,357	A→G	T20A (ACC→GCC)	yejF →	putative oligopeptide ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,276,357	0	A	G	100.0%	295.7 / NA	79	T20A (ACC→GCC)	yejF	putative oligopeptide ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/44);  total (35/44)

TGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGA  >  NC_000913/2276222‑2276493                                                                                                                                        |                                                                                                                                         tGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCcgt                                                                                                                                      <  2:72530/140‑1 (MQ=255) tGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCcgt                                                                                                                                      <  1:705849/140‑1 (MQ=255)   gCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCcgtac                                                                                                                                    >  1:304959/1‑140 (MQ=255)    cGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCcgtacg                                                                                                                                   >  1:634151/1‑140 (MQ=255)    cGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCcgtacg                                                                                                                                   <  2:130138/140‑1 (MQ=255)      atatTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCcgtacgta                                                                                                                                 <  2:873031/140‑1 (MQ=255)      atatTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCcgtacgta                                                                                                                                 <  1:229754/140‑1 (MQ=255)      atatTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCcgtacgta                                                                                                                                 <  1:719495/140‑1 (MQ=255)      atatTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCcgtacgta                                                                                                                                 <  2:405447/140‑1 (MQ=255)        attattGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACa                                                                                                                               <  2:634151/140‑1 (MQ=255)           attGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACagta                                                                                                                            >  2:691834/1‑140 (MQ=255)                   tGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAatga                                                                                                                    >  1:833754/1‑140 (MQ=255)                   tGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAatga                                                                                                                    >  1:650469/1‑140 (MQ=255)                   tGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAatga                                                                                                                    >  2:233574/1‑140 (MQ=255)                   tGCTGATCTTTATTGGTGAAGCCGGCCGCGACGCATTAGATCCTAATAAAGCAGTGTAGCATGTCGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAatga                                                                                                                    <  2:1101374/140‑1 (MQ=255)                     ctaaTCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAatgatg                                                                                                                  <  2:1072549/137‑1 (MQ=255)                        aTCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGttt                                                                                                               <  2:394476/140‑1 (MQ=255)                         tCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCTAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTc                                                                                                              >  1:498749/1‑140 (MQ=255)                         tCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAatga                                                                                                                    <  1:239108/134‑1 (MQ=255)                         tCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAatga                                                                                                                    >  2:239108/1‑134 (MQ=255)                         tCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTc                                                                                                              >  1:94187/1‑140 (MQ=255)                         tCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTc                                                                                                              <  1:233574/140‑1 (MQ=255)                         tCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTc                                                                                                              <  1:62405/140‑1 (MQ=255)                           tttATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCAc                                                                                                            >  1:772796/1‑140 (MQ=255)                             tATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAatga                                                                                                                    <  2:50284/130‑1 (MQ=255)                             tATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAatga                                                                                                                    >  1:50284/1‑130 (MQ=255)                             tATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTa                                                                                                          >  1:1134878/1‑140 (MQ=255)                                    gAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTg                                                                                                   >  1:392131/1‑140 (MQ=255)                                    gAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTg                                                                                                   >  2:268237/1‑140 (MQ=255)                                     aaGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGa                                                                                                  >  1:724811/1‑140 (MQ=255)                                       gCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAgg                                                                                                >  1:503430/1‑140 (MQ=255)                                       gCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAgg                                                                                                >  1:76451/1‑140 (MQ=255)                                       gCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAgg                                                                                                >  1:150330/1‑140 (MQ=255)                                            ccGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGc                                                                                           >  2:537471/1‑140 (MQ=255)                                                     aTTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTG‑AAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAAcgctgg                                                                                 <  1:438673/140‑1 (MQ=255)                                                      tttGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAAcgctgg                                                                                 <  2:143758/140‑1 (MQ=255)                                                      tttGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAAcgctgg                                                                                 <  1:1082899/140‑1 (MQ=255)                                                               aataaGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCtggtgg                                                                        <  1:846486/140‑1 (MQ=255)                                                                ataaGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTggtggg                                                                       <  2:833754/140‑1 (MQ=255)                                                                ataaGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTggtggg                                                                       <  2:1134878/140‑1 (MQ=255)                                                                 taaGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTggtgggt                                                                      >  1:1041192/1‑140 (MQ=255)                                                                 taaGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTggtgggt                                                                      <  2:76451/140‑1 (MQ=255)                                                                 taaGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTggtgggt                                                                      <  2:253440/140‑1 (MQ=255)                                                                 taaGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTggtgggg                                                                      >  2:616797/1‑139 (MQ=255)                                                                    ggCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAg                                                                   >  1:331237/1‑140 (MQ=255)                                                                     gCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCtggtgg                                                                        <  1:460083/134‑1 (MQ=255)                                                                     gCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCtggtgg                                                                        >  2:460083/1‑134 (MQ=255)                                                                     gCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTggtgggt                                                                      <  1:354137/136‑1 (MQ=255)                                                                     gCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTggtgggt                                                                      >  2:354137/1‑136 (MQ=255)                                                                     gCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGt                                                                  >  1:1125270/1‑140 (MQ=255)                                                                     gCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGt                                                                  >  1:436404/1‑140 (MQ=255)                                                                         tgtAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAgg                                                              >  1:1118845/1‑140 (MQ=255)                                                                          gtAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGt                                                             >  2:97195/1‑140 (MQ=255)                                                                          gtAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGt                                                             <  2:650469/140‑1 (MQ=255)                                                                                tGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGc                                                       <  1:268237/140‑1 (MQ=255)                                                                                   cGCTAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCaaa                                                    <  2:498749/140‑1 (MQ=255)                                                                                   cGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCaaa                                                    >  1:668181/1‑140 (MQ=255)                                                                                   cGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCaaa                                                    >  1:770445/1‑140 (MQ=255)                                                                                   cGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCaaa                                                    >  2:245786/1‑140 (MQ=255)                                                                                          tctGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTa                                             <  1:811422/140‑1 (MQ=255)                                                                                              ttAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACcgc                                         <  2:94187/140‑1 (MQ=255)                                                                                               tAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACcgcg                                        <  2:772796/140‑1 (MQ=255)                                                                                               tAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACcgcg                                        <  2:1125270/140‑1 (MQ=255)                                                                                                aGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCgcgc                                       <  1:371124/140‑1 (MQ=255)                                                                                                aGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCgcgc                                       <  1:173446/140‑1 (MQ=255)                                                                                                  cGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTa                                             <  1:291245/132‑1 (MQ=255)                                                                                                  cGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTACAGGCAAAAGCGTTa                                             >  2:291245/1‑132 (MQ=255)                                                                                                   gATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGt                                    <  2:770445/140‑1 (MQ=255)                                                                                                    aTTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTc                                   <  2:503430/140‑1 (MQ=255)                                                                                                    aTTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTc                                   >  1:131517/1‑140 (MQ=255)                                                                                                           aTTTGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTa                            <  2:150330/140‑1 (MQ=255)                                                                                                              tGTCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGc                         <  2:392131/140‑1 (MQ=255)                                                                                                                tCGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCt                       <  2:331237/140‑1 (MQ=255)                                                                                                                 cGGTGGGTTTTCGCCATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTg                      <  2:1041192/140‑1 (MQ=255)                                                                                                                              ccATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTGCCGCGCTGTCAATTTTACGCCTGCTCCCTTCCccgc         <  2:848482/140‑1 (MQ=255)                                                                                                                              ccATCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTGCCccgc         <  1:616797/140‑1 (MQ=255)                                                                                                                                 tCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCccgcc        >  1:321817/1‑138 (MQ=255)                                                                                                                                 tCAGCAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCccgcc        <  2:321817/138‑1 (MQ=255)                                                                                                                                     cAAGCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGa  >  1:234818/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                         TGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGA  >  NC_000913/2276222‑2276493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑