Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,338,468	T→C	N318S (AAC→AGC)	gyrA ←	DNA gyrase subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,338,468	0	T	C	100.0%	200.9 / NA	54	N318S (AAC→AGC)	gyrA	DNA gyrase subunit A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/23);  total (31/23)

GGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCACGCGTTTTTCTTTTACCAGTTCCGCAATC  >  NC_000913/2338332‑2338593                                                                                                                                         |                                                                                                                              ggTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGa                                                                                                                            >  1:365907/1‑140 (MQ=255)  gTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAg                                                                                                                           >  1:175459/1‑140 (MQ=255)    caccacTTCACGGTGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGca                                                                                                                         <  1:372875/140‑1 (MQ=255)    caccacTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGc                                                                                                                          >  2:446737/1‑139 (MQ=255)    caccacTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGc                                                                                                                          <  1:446737/139‑1 (MQ=255)            cACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCa                                                                                                                 >  1:1001088/1‑140 (MQ=255)            cACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGGTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCCGACCACAAATTcc                                                                                                                 >  2:749802/1‑139 (MQ=255)                 cggTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCaccgac                                                                                                            <  2:1001088/140‑1 (MQ=255)                    tGACGAACAAACGCCGCGATGATGTCTTTCAGGGTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGc                                                                                                         >  2:469784/1‑140 (MQ=255)                                  cgcgATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACtt                                                                                           <  2:175459/140‑1 (MQ=255)                                      atgatgTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAAt                                                                                       >  2:511235/1‑140 (MQ=255)                                        gatgTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCa                                                                                     <  1:6260/140‑1 (MQ=255)                                            tCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGt                                                                                                  <  1:129370/123‑1 (MQ=255)                                            tCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGt                                                                                                  >  2:129370/1‑123 (MQ=255)                                            tCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGAt                                                                                 >  2:380760/1‑140 (MQ=255)                                             cTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATg                                                                                <  1:511235/140‑1 (MQ=255)                                              tttCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCAc                                                                                    >  2:614136/1‑135 (MQ=255)                                              tttCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCAc                                                                                    <  1:614136/135‑1 (MQ=255)                                                 cAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCa                                                                            >  2:873991/1‑140 (MQ=255)                                                   ggTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATa                                                                          <  2:667588/140‑1 (MQ=255)                                                   ggTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATa                                                                          <  2:914419/140‑1 (MQ=255)                                                   ggTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATa                                                                          <  1:749802/140‑1 (MQ=255)                                                      tCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCg                                                                       >  2:1401/1‑140 (MQ=255)                                                      tCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCg                                                                       >  1:1087738/1‑140 (MQ=255)                                                       cATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGt                                                                      >  1:895397/1‑140 (MQ=255)                                                              ttCGGCTGACCATGGTGCAATGCTACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTc                                                               >  2:537351/1‑140 (MQ=255)                                                               tCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCa                                                              >  2:295182/1‑140 (MQ=255)                                                                cGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAg                                                             <  1:469784/140‑1 (MQ=255)                                                                cGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAg                                                             <  2:447102/140‑1 (MQ=255)                                                                cGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAg                                                             >  1:837721/1‑140 (MQ=255)                                                                      aCCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAg                                                             >  2:649594/1‑134 (MQ=255)                                                                      aCCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAg                                                             <  1:649594/134‑1 (MQ=255)                                                                               cAATGCTACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAgcg                                              <  1:537351/140‑1 (MQ=255)                                                                                 aTGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAgcgcg                                            <  2:365907/140‑1 (MQ=255)                                                                                            ttGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTc                                 >  2:413924/1‑140 (MQ=255)                                                                                            ttGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTc                                 >  2:530641/1‑140 (MQ=255)                                                                                                 aCCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCAcgc                            >  2:662120/1‑140 (MQ=255)                                                                                                  ccGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCAcgcg                           >  2:419884/1‑140 (MQ=255)                                                                                                  ccGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCAcgcg                           >  2:538011/1‑140 (MQ=255)                                                                                                    gaaagaaaCCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCACGCGtt                         >  2:813662/1‑140 (MQ=255)                                                                                                    gaaagaaaCCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCACGCGtt                         <  2:892589/140‑1 (MQ=255)                                                                                                     aaagaaaCCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCACGCGttt                        >  2:1132523/1‑140 (MQ=255)                                                                                                      aagaaaCCTGCAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCACGCGtttt                       >  1:354615/1‑140 (MQ=255)                                                                                                                cAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCACGCGtt                         <  2:883718/128‑1 (MQ=255)                                                                                                                cAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCACGCGtt                         >  1:883718/1‑128 (MQ=255)                                                                                                                cAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCACGCGTTTTTCTTTTACCa             >  1:716850/1‑140 (MQ=255)                                                                                                                cAACTGGGTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCACGCGTTTTTCTTTTACCa             >  2:1039594/1‑140 (MQ=255)                                                                                                                      ggTCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCACGCGTTTTTCTTTTACCAGTTCCg       <  1:19142/140‑1 (MQ=255)                                                                                                                        tCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCACGCGTTTTTCTTTTACCAGTTCCGCa     <  1:295182/140‑1 (MQ=255)                                                                                                                        tCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCACGCGTTTTTCTTTTACCAGTTCCGCa     <  1:742534/140‑1 (MQ=255)                                                                                                                        tCTGGGAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCACGCGTTTTTCTTTTACCAGTTCCGCa     >  1:932850/1‑140 (MQ=255)                                                                                                                           gggAGTAGAGGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCACGCGTTTTTCTTTTACCAGTTCCGCAAtc  >  1:834578/1‑140 (MQ=255)                                                                                                                                 agagGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCACGCGTTTTTCTTTTACCAGTTCCGCa     <  1:435267/131‑1 (MQ=255)                                                                                                                                 agagGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCACGCGTTTTTCTTTTACCAGTTCCGCa     <  1:373915/131‑1 (MQ=255)                                                                                                                                 agagGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCACGCGTTTTTCTTTTACCAGTTCCGCa     >  2:435267/1‑131 (MQ=255)                                                                                                                                 agagGTTGCTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCACGCGTTTTTCTTTTACCAGTTCCGCa     >  2:373915/1‑131 (MQ=255)                                                                                                                                         |                                                                                                                              GGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCACGCGTTTTTCTTTTACCAGTTCCGCAATC  >  NC_000913/2338332‑2338593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑