Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,375,217	A→G	V249A (GTA→GCA)	menC ←	o‑succinylbenzoate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,375,217	0	A	G	100.0%	206.5 / NA	56	V249A (GTA→GCA)	menC	o‑succinylbenzoate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/34);  total (22/34)

CAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGCTCGCGCAGGCTTTCATCCCAGGCAATGGCAATGCCGGTTTCA  >  NC_000913/2375087‑2375351                                                                                                                                   |                                                                                                                                       cAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTcgc                                                                                                                               <  1:703493/140‑1 (MQ=255)  aGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTcgcg                                                                                                                              >  1:379246/1‑140 (MQ=255)   gCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGt                                                                                                                             <  1:134767/140‑1 (MQ=255)   gCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGt                                                                                                                             >  1:152846/1‑140 (MQ=255)    cGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTa                                                                                                                            >  2:782994/1‑140 (MQ=255)      tgtCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACt                                                                                                                          >  1:858840/1‑140 (MQ=255)        tCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGTAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACttt                                                                                                                        >  2:750252/1‑140 (MQ=255)        tCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACttt                                                                                                                        >  1:616952/1‑140 (MQ=255)         ccAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACtttt                                                                                                                       <  2:685293/140‑1 (MQ=255)             cccTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCa                                                                                                                   <  2:616952/140‑1 (MQ=255)               cTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGa                                                                                                                 <  2:230037/140‑1 (MQ=255)               cTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGa                                                                                                                 <  1:750252/140‑1 (MQ=255)                 ggAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACt                                                                                                               <  2:363020/140‑1 (MQ=255)                  gAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTg                                                                                                              >  2:802136/1‑140 (MQ=255)                     tGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTGccc                                                                                                           <  2:991739/140‑1 (MQ=255)                       gtgtCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACtttt                                                                                                                       <  2:278190/126‑1 (MQ=255)                       gtgtCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACtttt                                                                                                                       >  1:278190/1‑126 (MQ=255)                       gtgtCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGt                                                                                                         >  2:181041/1‑140 (MQ=255)                       gtgtCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGt                                                                                                         <  1:181041/140‑1 (MQ=255)                       gtgtCCGGCGTTAACCAGGCGGCAACCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGt                                                                                                         >  1:41458/1‑140 (MQ=255)                        tgtCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGTg                                                                                                        <  1:594167/140‑1 (MQ=255)                            cGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCg                                                                                                    <  1:808953/140‑1 (MQ=255)                            cGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCg                                                                                                    >  2:690507/1‑140 (MQ=255)                            cGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCg                                                                                                    >  1:901868/1‑140 (MQ=255)                            cGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCg                                                                                                    <  2:632809/140‑1 (MQ=255)                             ggCGTTAACCAGGCGGCAATCCGCGCCAGTGGCGTTAAGCCTAAGCGCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGt                                                                                                   <  2:705798/140‑1 (MQ=255)                                 ttAACCAGGCGGCAACCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGt                                                                                               <  2:792680/140‑1 (MQ=255)                                 tcagccAGGCGGCAACCCGCGCCAGTTCCGTTAAGCCTAAGCTCGATTCAATGAAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGt                                                                                               <  2:1096996/136‑1 (MQ=255)                                         gcggcAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAAc                                                                                       >  2:809078/1‑140 (MQ=255)                                                tCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACcgcg                                                                                <  2:780896/140‑1 (MQ=255)                                                    cgcCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCgcgcgcc                                                                            >  1:1079736/1‑139 (MQ=255)                                                      ccAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACg                                                                          <  1:782994/140‑1 (MQ=255)                                                      ccAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACg                                                                          <  1:690507/140‑1 (MQ=255)                                                       cAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGc                                                                         <  1:304154/140‑1 (MQ=255)                                                       cAGTTGCGTTAAGCCTAAGCGCGATACAAGGGAAGAACTGGTCGCCGCCGTCAGCCGCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGc                                                                         <  2:1018632/140‑1 (MQ=255)                                                                 aaGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTc                                                                  >  1:64252/1‑137 (MQ=255)                                                                 aaGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTc                                                                  <  2:64252/137‑1 (MQ=255)                                                                        aGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAg                                                        >  1:765271/1‑140 (MQ=255)                                                                              aTTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCaaaa                                                  >  1:1124862/1‑140 (MQ=255)                                                                                   aTGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCgg                                             <  2:379246/140‑1 (MQ=255)                                                                                    tGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGc                                            <  2:858840/140‑1 (MQ=255)                                                                                    tGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGc                                            >  1:710131/1‑140 (MQ=255)                                                                                          accTGATCCCCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGCTCgcgc                                      <  2:286521/138‑1 (MQ=255)                                                                                          aaCTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGCTCgcgc                                      <  1:520466/140‑1 (MQ=255)                                                                                          aaCTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGCTCgcgc                                      <  2:74880/140‑1 (MQ=255)                                                                                           aCTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGCTCGCGCa                                     <  2:765271/140‑1 (MQ=255)                                                                                             tGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGCTCGCGCAgg                                   >  2:472385/1‑140 (MQ=255)                                                                                                      ccgTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGCTCGCGCAGGCTTTCATcc                          >  2:253519/1‑140 (MQ=255)                                                                                                      ccgTCAGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGCTCGCGCAGGCTTTCATcc                          <  1:472385/140‑1 (MQ=255)                                                                                                           aGCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGCTCGCGCAGGCTTTCATCCCAggc                     <  2:152846/140‑1 (MQ=255)                                                                                                            gCCCCAGCGCGTGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGCTCGCGCAGGCTTTCATCCCAggca                    <  1:802136/140‑1 (MQ=255)                                                                                                                       tGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGCTCGCGCAGGCTTTCATCCCAggca                    >  1:821163/1‑129 (MQ=255)                                                                                                                       tGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGCTCGCGCAGGCTTTCATCCCAggca                    <  2:821163/129‑1 (MQ=255)                                                                                                                       tGCGCCGCCTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGCTCGCGCAGGCTTTCATCCCAGGCAATGGCAATGcc         >  2:340195/1‑140 (MQ=255)                                                                                                                             gccTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGCTCGCGCAGGCTTTCATCCCAGGCAATGGCAATGCCGGTTTc   <  1:809078/140‑1 (MQ=255)                                                                                                                              ccTGTGCCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGCTCGCGCAGGCTTTCATCCCAGGCAATGGCAATGCCGGTTTCa  <  2:710131/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                       CAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGCTCGCGCAGGCTTTCATCCCAGGCAATGGCAATGCCGGTTTCA  >  NC_000913/2375087‑2375351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑