Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,426,387	T→C	K134K (AAA→AAG)	hisJ ←	histidine ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,426,387	0	T	C	100.0%	135.1 / NA	37	K134K (AAA→AAG)	hisJ	histidine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/12);  total (25/12)

CGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGA  >  NC_000913/2426251‑2426523                                                                                                                                         |                                                                                                                                         cgGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGCTTg                                                                                                                                       >  2:1067612/1‑140 (MQ=255) cgGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGCTTg                                                                                                                                       >  1:857422/1‑140 (MQ=255)  ggCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGAGGTGCCCTGCAATACGCCTACCCGCTTGc                                                                                                                                      >  1:1091565/1‑140 (MQ=255)                               aaTAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGCTTGCCTTTCAGCGACTCGACTGTCGGCTGAATg                                                                                                         >  2:672891/1‑140 (MQ=255)                                    aTGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGCTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGa                                                                                                    >  2:619612/1‑140 (MQ=255)                                    aTGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGCTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGa                                                                                                    >  1:683435/1‑140 (MQ=255)                                        tgtCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGCTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAAttt                                                                                                >  1:732595/1‑140 (MQ=255)                                         gtCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGCTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAAtttt                                                                                               >  1:146800/1‑140 (MQ=255)                                         gtCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGCTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAAtttt                                                                                               <  1:672891/140‑1 (MQ=255)                                              gCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATCCGCCTACCCGCTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGcc                                                                                          <  2:510081/140‑1 (MQ=255)                                                  cTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGCTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGccacca                                                                                      <  2:683435/140‑1 (MQ=255)                                                  cTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGCTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGccacca                                                                                      <  1:619612/140‑1 (MQ=255)                                                          agaCGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGCTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGc                                                                                           <  2:451553/127‑1 (MQ=255)                                                          agaCGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGCTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGc                                                                                           >  1:451553/1‑127 (MQ=255)                                                          agaCGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGCTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACga                                                                              >  1:908946/1‑140 (MQ=255)                                                               aTTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGCTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATc                                                                         >  1:929984/1‑140 (MQ=255)                                                                tttCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGCTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCg                                                                        >  1:18653/1‑140 (MQ=255)                                                                     aTGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGCTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGc                                                                   >  1:123256/1‑140 (MQ=255)                                                                      tGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGCTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCg                                                                  <  2:857422/140‑1 (MQ=255)                                                                        ccTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGCTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTa                                                                <  2:146800/140‑1 (MQ=255)                                                                                  cccAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGCTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCg                                                      >  2:724101/1‑140 (MQ=255)                                                                                     aaTGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGCTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTg                                                   >  1:409226/1‑140 (MQ=255)                                                                                               aCCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGCTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTAttt                                         >  1:169055/1‑140 (MQ=255)                                                                                                 cGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTGCCCGCTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCt                                       >  2:1130341/1‑140 (MQ=255)                                                                                                 cGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGCTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCt                                       >  1:582621/1‑140 (MQ=255)                                                                                                   aaCGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGCTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTg                                     >  1:1006390/1‑140 (MQ=255)                                                                                                       gctcCTGGGTGGTGTCCTGCAATACGCGTACCCGCTTGGCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGa                                 >  2:844859/2‑140 (MQ=255)                                                                                                                  gtgCCCTGCAATACGCCTACCCGCTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGt                      >  1:233750/1‑140 (MQ=255)                                                                                                                        tGCAATACGCCTACCCGCTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCCGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGa                >  1:337074/1‑140 (MQ=255)                                                                                                                            aTACGCCTACCCGCTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCCGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGc            <  2:337074/140‑1 (MQ=255)                                                                                                                            aTACGCCTACCCGCTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGc            <  2:169055/140‑1 (MQ=255)                                                                                                                             tACGCCTACCCGCTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCg           >  1:996152/1‑140 (MQ=255)                                                                                                                              aCGCCTACCCGCTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGa          <  2:650540/140‑1 (MQ=255)                                                                                                                                  cTACCCGCTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGAc      >  1:134755/1‑140 (MQ=255)                                                                                                                                    aCCCGCTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACat    >  1:670111/1‑140 (MQ=255)                                                                                                                                    aCCCGCTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACat    >  2:1050313/1‑140 (MQ=255)                                                                                                                                      ccGCTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACatga  <  2:908946/140‑1 (MQ=255)                                                                                                                                      ccGCTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACatga  <  2:409226/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGA  >  NC_000913/2426251‑2426523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑