Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,429,397	G→A	T281M (ACG→ATG)	purF ←	amidophosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,429,397	0	G	A	100.0%	180.1 / NA	51	T281M (ACG→ATG)	purF	amidophosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/28);  total (23/28)

AACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGCACATTGACGGGTAAACAACTGCCCTTC  >  NC_000913/2429261‑2429533                                                                                                                                         |                                                                                                                                         aaCGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGc                                                                                                                                       <  2:396172/140‑1 (MQ=255)   cGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGccc                                                                                                                                     <  1:953785/140‑1 (MQ=255)       gCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATg                                                                                                                                        >  2:97077/1‑133 (MQ=255)        cccTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATAtt                                                                                                                                <  1:36477/140‑1 (MQ=255)          cTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGAGCAATTTTCTCGCCCAGTTTCATGCCCATATTca                                                                                                                              <  1:137012/140‑1 (MQ=255)            ggCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTcaca                                                                                                                            <  1:16026/140‑1 (MQ=255)                gTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACAcgcg                                                                                                                        <  1:1004048/140‑1 (MQ=255)                       ttGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTa                                                                                                                 >  1:381880/1‑140 (MQ=255)                                         aTTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGAt                                                                                               >  1:393907/1‑140 (MQ=255)                                         aTTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGAt                                                                                               >  2:626964/1‑140 (MQ=255)                                                      tatCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCcggg                                                                                  >  2:1018796/1‑140 (MQ=255)                                                      tatCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCcggg                                                                                  >  2:256564/1‑140 (MQ=255)                                                         cacaCGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCcgggcgg                                                                               >  1:281140/1‑140 (MQ=255)                                                          acacGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCcgggcggg                                                                              <  1:1018796/140‑1 (MQ=255)                                                          acacGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCcgggcggg                                                                              <  1:869310/140‑1 (MQ=255)                                                           cacGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCgggcgggc                                                                             <  1:256564/140‑1 (MQ=255)                                                            acGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCa                                                                            <  2:281140/140‑1 (MQ=255)                                                             cGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCaa                                                                           <  2:256291/140‑1 (MQ=255)                                                              gAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCaaa                                                                          >  1:204950/1‑140 (MQ=255)                                                               aGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAg                                                                         <  2:381880/140‑1 (MQ=255)                                                                ggTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCcgg                                                                                   <  1:150157/129‑1 (MQ=255)                                                                ggTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCcgg                                                                                   >  2:150157/1‑129 (MQ=255)                                                                ggTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGt                                                                        >  2:285833/1‑140 (MQ=255)                                                                     cTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTataca                                                                   <  2:393907/140‑1 (MQ=255)                                                                      tGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTatacat                                                                  >  2:453435/1‑140 (MQ=255)                                                                            cgggatcACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCaa                                                            >  2:775749/1‑140 (MQ=255)                                                                             gggatcACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCaaa                                                           <  1:219378/140‑1 (MQ=255)                                                                              ggatcACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAAc                                                          >  2:399044/1‑140 (MQ=255)                                                                                  caccacGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGc                                                      >  2:937085/1‑140 (MQ=255)                                                                                       cGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGa                                                 >  2:55502/1‑140 (MQ=255)                                                                                       cGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGa                                                 >  2:1048722/1‑140 (MQ=255)                                                                                         tCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGAtt                                               >  2:1014576/1‑140 (MQ=255)                                                                                                   gATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGa                                     >  2:895873/1‑140 (MQ=255)                                                                                                    aTCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGAt                                    >  2:809921/1‑140 (MQ=255)                                                                                                       ttCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGt                                 <  2:757258/140‑1 (MQ=255)                                                                                                        tCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTc                                >  2:462398/1‑140 (MQ=255)                                                                                                                gcgGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGCACAtt                        <  2:380048/140‑1 (MQ=255)                                                                                                                   ggCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGCACATTGAc                     >  1:829419/1‑140 (MQ=255)                                                                                                                   ggCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGCACATTGAc                     >  2:224130/1‑140 (MQ=255)                                                                                                                   ggCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGCACATTGAc                     <  1:1048722/140‑1 (MQ=255)                                                                                                                        ttttCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTc                                <  1:702298/124‑1 (MQ=255)                                                                                                                        ttttCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTc                                >  2:702298/1‑124 (MQ=255)                                                                                                                        ttttCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGCACATTGACGGGTa                <  1:55502/140‑1 (MQ=255)                                                                                                                           tctcGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGCACATTGACGGGTAaac             <  1:1014576/140‑1 (MQ=255)                                                                                                                                cccAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGCACATTGACGGGTAAACAACTg        <  1:626964/140‑1 (MQ=255)                                                                                                                                   aGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGCACATTGACGGGTAAACAACTGccc     <  2:204950/140‑1 (MQ=255)                                                                                                                                   aGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGCACATTGACGGGTAAACAACTGccc     <  1:930467/140‑1 (MQ=255)                                                                                                                                   aGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGCACATTGACGGGTAAACAACTGccc     <  1:399044/140‑1 (MQ=255)                                                                                                                                   aGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGCACATTGACGGGTAAACAACTGccc     <  2:991062/140‑1 (MQ=255)                                                                                                                                     tttCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGCACATTGACGGGTAAACAACTGCCctt   <  1:285833/140‑1 (MQ=255)                                                                                                                                      ttCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGCACATTGACGGGTAAACAACTGCCcttc  <  1:809921/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         AACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGCACATTGACGGGTAAACAACTGCCCTTC  >  NC_000913/2429261‑2429533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑