Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	308,745	C→T	S196S (TCG→TCA)	ecpC ←	putative fimbrial usher protein EcpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	308,745	0	C	T	100.0%	131.0 / NA	37	S196S (TCG→TCA)	ecpC	putative fimbrial usher protein EcpC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/3);  total (34/3)

CCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTACGG  >  NC_000913/308608‑308878                                                                                                                                          |                                                                                                                                      ccGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACG‑TATTCAGTGAc                                                                                                                                    <  2:605019/140‑1 (MQ=255)      aaaTCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGTGACAGAt                                                                                                                                <  1:684044/140‑1 (MQ=255)                       aaTCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGTGACAGATAGCTGGATGTGTTGCTc                                                                                                               >  1:611586/1‑140 (MQ=255)                                       cGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGTGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACt                                                                                               >  1:115932/1‑140 (MQ=255)                                         ttttATATATTTCCCTGTCTTGTTCCCCGCCCCCGCCCCCGAACAGCGACCCGCCGACCACCAAATGTTTTCCGCGCAGTGCAGTATTTTTTTTAAGAGACAGATAGCTGGTTGTTTCCCTCCCGCCGTTACGCAACTgg                                                                                             <  2:1125986/139‑1 (MQ=255)                                         cTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGTGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTgg                                                                                             >  2:715166/1‑140 (MQ=255)                                         cTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGTGACAGAAAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTgg                                                                                             >  1:531020/1‑140 (MQ=255)                                           ttATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGTGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGgtt                                                                                           >  1:166906/1‑140 (MQ=255)                                            tatataATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGTGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGgttg                                                                                          >  1:869342/1‑140 (MQ=255)                                             atataATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGTGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGgttgt                                                                                         >  1:906314/1‑140 (MQ=255)                                             atataATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGTGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGgttgt                                                                                         >  1:1125986/1‑140 (MQ=255)                                                 aaTTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGTGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTtata                                                                                     >  2:181219/1‑140 (MQ=255)                                                   ttCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGTGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGa                                                                                   >  1:896747/1‑140 (MQ=255)                                                   ttCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGTGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGa                                                                                   >  1:562271/1‑140 (MQ=255)                                                        tGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGTGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAgctgtct                                                                              >  1:26442/1‑134 (MQ=255)                                                        tGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGTGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGccc                                                                              >  2:599019/1‑140 (MQ=255)                                                                    cGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGTGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTc                                                                  >  2:486543/1‑140 (MQ=255)                                                                    cGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGTGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTc                                                                  >  2:43235/1‑140 (MQ=255)                                                                      cTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGTGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAg                                                                >  1:470516/1‑140 (MQ=255)                                                                            aTCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGTGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGAttctg                                                          >  1:1060459/1‑137 (MQ=255)                                                                                cgtccggCGGGCCGGCGACCCCCACATGTTGTTCGCGAAGGGCAGTATCTTTATTAAGTGAAAGATAGGTGGTTGTTTTCCCCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTActgctg                                                      <  2:359706/134‑1 (MQ=255)                                                                                 gTACGGCGCGCCGGCGAACACCACTGGTTGTCCGGGAAGTGAATTAATTTTATTAAGTGAGAAATAGGGGTTTGTGTTGCCCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGa                                                     <  2:533198/140‑1 (MQ=255)                                                                                  tACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGTGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAg                                                    >  2:305537/1‑140 (MQ=255)                                                                                  aaCAGGGCGCCGCCGACCACCACATGATGTTCGCGCGGTGCAGTATCTTTATTCGGAAAAAGATAGTGGGTTGTGTTCCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAg                                                    <  2:1059522/139‑1 (MQ=255)                                                                                   aCAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGTGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAgg                                                   >  1:533198/1‑140 (MQ=255)                                                                                     ggCGACCCGCCGAGCCCAAAATGTTGTTGGCGCAGTGCAGTAACTTTATTCAGTGAAAGATGGGGGGTTGTGTTGCCCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGgt                                                 <  2:906314/139‑1 (MQ=255)                                                                                       gggcccgccgACCACCACATGTTTTGCGCGCGGTGCAGTAATTTTATTCAGTGAAAGATGGTGGGTTGTGTTCCCCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGgtgt                                               <  2:869342/136‑1 (MQ=255)                                                                                       cGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGTGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGgtgt                                               >  1:1059522/1‑140 (MQ=255)                                                                                       cGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGTGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGgtgt                                               >  1:201498/1‑140 (MQ=255)                                                                                         aGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGTGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTa                                             >  1:494433/1‑140 (MQ=255)                                                                                                        caTGATGTTCGCGCAGTGCAGTAACGTTATTCAGTGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCg                              >  1:981172/1‑140 (MQ=255)                                                                                                           gatgTTCGCGCAGTGCAGTAACGTTATTCAGTGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATg                           >  1:299265/1‑140 (MQ=255)                                                                                                                tCGCGCAGTGCAGTAACGTTATTCAGTGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAGCACTGGACTGCCCGATGTCTTc                      >  1:803616/1‑140 (MQ=255)                                                                                                                tCGCGCAGTGCAGTAACGTTATTCAGTGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTc                      >  2:43694/1‑140 (MQ=255)                                                                                                                  gcgcAGTGCAGTAACGTTATTCAGTGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGc                    >  1:1042304/1‑140 (MQ=255)                                                                                                                          aagtATCTTTATTCAGGGACAGATGGTTGGTTGTGTTCCCCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAg            <  2:76212/139‑1 (MQ=255)                                                                                                                            ttatttttATTCAGTGACAGATGGCGGGTTGTGTTCCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCg          <  2:488675/134‑1 (MQ=255)                                                                                                                                  ttATTCAGTGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTAc    >  1:488675/1‑140 (MQ=255)                                                                                                                                  ttATTCAGTGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTAc    >  1:466873/1‑140 (MQ=255)                                                                                                                                  ttATTCAGTGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTAc    >  1:658657/1‑140 (MQ=255)                                                                                                                                  ttATTCAGTGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTAc    >  1:222908/1‑140 (MQ=255)                                                                                                                                  ttATTCAGTGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTAc    >  2:93906/1‑140 (MQ=255)                                                                                                                                  ttATTCAGTGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTAc    >  2:963488/1‑140 (MQ=255)                                                                                                                                   tATTCAGTGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTACg   <  1:181219/140‑1 (MQ=255)                                                                                                                                   tATTCAGTAAAAGATAGGTGGTTGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTACg   <  2:896747/140‑1 (MQ=255)                                                                                                                                    aTTCAGTGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTACgg  >  1:76212/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      CCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTACGG  >  NC_000913/308608‑308878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑