Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,432,226	G→A	S239S (AGC→AGT)	folC ←	bifunctional folylpolyglutamate synthetase/dihydrofolate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,432,226	0	G	A	100.0%	176.9 / NA	51	S239S (AGC→AGT)	folC	bifunctional folylpolyglutamate synthetase/dihydrofolate synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/33);  total (18/33)

TGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCACCGACAATTGCCGGTTTTTCG  >  NC_000913/2432091‑2432361                                                                                                                                        |                                                                                                                                        tGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTaa                                                                                                                                     <  1:379211/140‑1 (MQ=255)    gcTGGCAATCCCGTCGCGAATGGCGCTTTCAATGCCTTCCAGCCGGCTGGCACGCAGTGCGGCCAGCGCTGTTGCGGCATTCGGCTGCGGGCCACGCGGCCACGGCCGAGTTTCCCGCCTGCCGTGAGCATCGCTATAcc                                                                                                                                  <  2:160749/140‑2 (MQ=255)    gcTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACg                                                                                                                                  <  1:876202/140‑1 (MQ=255)    gcTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACg                                                                                                                                  <  2:615138/140‑1 (MQ=255)    gcTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACg                                                                                                                                  <  2:286187/140‑1 (MQ=255)     cTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACGc                                                                                                                                 <  2:717217/140‑1 (MQ=255)     cTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACGc                                                                                                                                 <  1:398113/140‑1 (MQ=255)      tGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACGcc                                                                                                                                <  1:714173/140‑1 (MQ=255)      tGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACGcc                                                                                                                                <  2:219655/140‑1 (MQ=255)                gTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACGCCCAGTCATGAt                                                                                                                      <  2:1097498/140‑1 (MQ=255)                  cgcgAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACGCCCAGTCATGATCg                                                                                                                    >  2:5997/1‑140 (MQ=255)                  cgcgAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAAC‑CCCAGTCATGATCgg                                                                                                                   >  2:853897/1‑140 (MQ=255)                   gcgAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACGCCCAGTCATGATCgg                                                                                                                   <  2:448983/140‑1 (MQ=255)                     gAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAAC‑CCCAGTCATGATCGGTGa                                                                                                                <  1:853897/140‑1 (MQ=255)                      aaTGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACg                                                                                                                                  <  2:167773/122‑1 (MQ=255)                      aaTGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACg                                                                                                                                  >  1:167773/1‑122 (MQ=255)                                     gACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACt                                                                                                 >  2:115930/1‑140 (MQ=255)                                         tCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAAc                                                                                             >  2:1103367/1‑140 (MQ=255)                                              cccGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACGCCCGGTCATGATCGGTGACGGAATAGTTCCACTCAACGcccc                                                                                        <  2:236357/140‑1 (MQ=255)                                              cccGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGcccc                                                                                        <  1:329137/140‑1 (MQ=255)                                                cGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGa                                                                                      <  1:36407/140‑1 (MQ=255)                                                cGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGa                                                                                      <  1:754221/140‑1 (MQ=255)                                                cGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGa                                                                                      >  2:188357/1‑140 (MQ=255)                                                cGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACGCACAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGa                                                                                      <  1:504722/140‑1 (MQ=255)                                                  cTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACg                                                                                    >  1:730228/1‑140 (MQ=255)                                                    ggCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGtt                                                                                  >  2:873555/1‑140 (MQ=255)                                                                cgccAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCAc                                                                      >  2:199424/1‑140 (MQ=255)                                                                  ccAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCt                                                                    <  1:117382/140‑1 (MQ=255)                                                                  ccAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCt                                                                    <  1:84538/140‑1 (MQ=255)                                                                           ttGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTgc                                                           <  1:359523/140‑1 (MQ=255)                                                                            tGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCCTTTTCCTgcg                                                          <  1:205772/140‑1 (MQ=255)                                                                                gCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCcac                                                      >  1:282936/1‑140 (MQ=255)                                                                                 cATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCcaca                                                     <  1:199424/140‑1 (MQ=255)                                                                                  aTTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACGCCCAGTCATGATCGGTCACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACAt                                                    <  2:1015334/140‑1 (MQ=255)                                                                                   ttCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATc                                                   <  2:900476/140‑1 (MQ=255)                                                                                   ttCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATc                                                   <  2:282936/140‑1 (MQ=255)                                                                                   ttCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATc                                                   >  1:240081/1‑140 (MQ=255)                                                                                        ttGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCaa                                              <  2:274915/140‑1 (MQ=255)                                                                                         tGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAAt                                             <  2:730228/140‑1 (MQ=255)                                                                                           cGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGTCGTGAGCATCACTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATgg                                           >  1:356628/1‑140 (MQ=255)                                                                                             ggACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAg                                                 >  1:914891/1‑132 (MQ=255)                                                                                             ggACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAg                                                 <  2:914891/132‑1 (MQ=255)                                                                                                cAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATGGTAGaa                                      >  2:36727/1‑140 (MQ=255)                                                                                                           ggCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCgg                           >  1:245720/1‑140 (MQ=255)                                                                                                           ggCAGATTTTCCAGCGTGCCGTGAGCATCACTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCgg                           <  2:84515/140‑1 (MQ=255)                                                                                                                aTTTTCCAGCGTGCCGTGAGCATCACTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATGGTAGAAg                                     <  2:598349/125‑1 (MQ=255)                                                                                                                aTTTTCCAGCGTGCCGTGAGCATCACTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATGGTAGAAg                                     >  1:598349/1‑125 (MQ=255)                                                                                                                         cGTGCCGTGAGCATCACTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCACCGACAATTg             >  2:289174/1‑140 (MQ=255)                                                                                                                          gTGCCGTGAGCATCACTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCACCGACAATTGc            <  1:1103367/140‑1 (MQ=255)                                                                                                                            gCCGTGAGCATCACTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCACCGACAATTGCCg          >  2:433122/1‑140 (MQ=255)                                                                                                                                    cATCACTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCACCGACAATTGCCGGTTTTTCg  <  2:240081/140‑1 (MQ=255)                                                                                                                                    cATCACTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCACCGACAATTGCCGGTTTTTCg  <  1:873555/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        TGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCACCGACAATTGCCGGTTTTTCG  >  NC_000913/2432091‑2432361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑