Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,656,054	T→C	N102S (AAC→AGC)	pepB ←	aminopeptidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,656,054	0	T	C	100.0%	219.5 / NA	59	N102S (AAC→AGC)	pepB	aminopeptidase B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/26);  total (33/26)

ATAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACAT  >  NC_000913/2655927‑2656186                                                                                                                                |                                                                                                                                     aTAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCAAGTTCCt                                                                                                                          >  1:294341/1‑140 (MQ=255)           cGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGc                                                                                                                >  1:519878/1‑140 (MQ=255)            gATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCa                                                                                                               <  2:356661/140‑1 (MQ=255)               cGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCa                                                                                                               >  1:507749/1‑137 (MQ=255)               cGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCa                                                                                                               <  2:507749/137‑1 (MQ=255)               cGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCg                                                                                                            >  2:1029630/1‑140 (MQ=255)                gCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGt                                                                                                           >  1:98445/1‑140 (MQ=255)                        cGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATcc                                                                                                   >  1:26454/1‑140 (MQ=255)                                   agatcaACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCAcaccac                                                                                        >  2:156753/1‑140 (MQ=255)                                     atcaACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACtt                                                                                      >  1:92238/1‑140 (MQ=255)                                          cAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCgg                                                                                                 <  2:177345/124‑1 (MQ=255)                                          cAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCgg                                                                                                 >  1:177345/1‑124 (MQ=255)                                          cAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCcaca                                                                                            <  1:186639/129‑1 (MQ=255)                                          cAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCcaca                                                                                            >  2:186639/1‑129 (MQ=255)                                          cAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTAcgc                                                                                 >  2:884848/1‑140 (MQ=255)                                          cAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTAcgc                                                                                 >  1:499158/1‑140 (MQ=255)                                            gCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGt                                                                               >  2:51304/1‑140 (MQ=255)                                             cACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTg                                                                              >  2:36596/1‑140 (MQ=255)                                               cGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGcc                                                                            >  1:736517/1‑140 (MQ=255)                                               cGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGcc                                                                            >  1:397424/1‑140 (MQ=255)                                                    tgCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCttt                                                                         <  1:470061/138‑1 (MQ=255)                                                    tgCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCttt                                                                         >  2:470061/1‑138 (MQ=255)                                                     gCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCgg                                                                      <  2:229164/140‑1 (MQ=255)                                                     gCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCgg                                                                      <  1:264253/140‑1 (MQ=255)                                                     gCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCgg                                                                      >  1:661510/1‑140 (MQ=255)                                                      ccAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCggg                                                                     <  1:364943/140‑1 (MQ=255)                                                      ccAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCAAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCggg                                                                     <  2:294341/140‑1 (MQ=255)                                                            gCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTg                                                               >  1:228387/1‑140 (MQ=255)                                                             cGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGt                                                              <  2:1010004/140‑1 (MQ=255)                                                               aTGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTaa                                                            <  1:884848/140‑1 (MQ=255)                                                                 ggTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAAcc                                                          >  1:12991/1‑140 (MQ=255)                                                                     ccAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGc                                                      >  1:391820/1‑140 (MQ=255)                                                                         ttcttcTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGa                                                  <  1:725225/140‑1 (MQ=255)                                                                         ttcttcTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGa                                                  <  1:221083/140‑1 (MQ=255)                                                                         ttcttcTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCAGGGCTTTGTAACCTTGCCAGa                                                  <  1:744218/140‑1 (MQ=255)                                                                             tctGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGc                                              >  2:111878/1‑140 (MQ=255)                                                                                 ccGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCcag                                          >  2:860721/1‑140 (MQ=255)                                                                                   ggTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCcagca                                        >  1:482520/1‑140 (MQ=255)                                                                                    gTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCcagcag                                       <  1:581393/140‑1 (MQ=255)                                                                                     tGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCagcagc                                      <  2:12991/140‑1 (MQ=255)                                                                                      gCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCg                                     <  2:26454/140‑1 (MQ=255)                                                                                               gtgtCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCg                                <  1:1107692/136‑1 (MQ=255)                                                                                               gtgtCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCg                                >  2:1107692/1‑136 (MQ=255)                                                                                               gtgtCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATc                            <  1:649885/140‑1 (MQ=255)                                                                                                 gtCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATccc                          >  1:588938/1‑140 (MQ=255)                                                                                                      gCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCt                     >  1:287412/1‑140 (MQ=255)                                                                                                       cACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCtt                    >  2:1048761/1‑140 (MQ=255)                                                                                                       cACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCtt                    <  2:397424/140‑1 (MQ=255)                                                                                                        ccccAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTc                   <  2:391820/139‑1 (MQ=255)                                                                                                          ccAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCAc                 >  2:678536/1‑140 (MQ=255)                                                                                                            aGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCAGCCCAGCCTTCACCg               >  2:697039/1‑140 (MQ=255)                                                                                                                 atgatCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTaa          <  1:1129582/140‑1 (MQ=255)                                                                                                                        tcaGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCg               >  1:551021/1‑128 (MQ=255)                                                                                                                        tcaGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCg               <  2:551021/128‑1 (MQ=255)                                                                                                                        tcaGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACa   >  1:348392/1‑140 (MQ=255)                                                                                                                        tcaGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACa   >  1:711099/1‑140 (MQ=255)                                                                                                                        tcaGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACa   <  1:156753/140‑1 (MQ=255)                                                                                                                        tcaGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACa   <  2:92238/140‑1 (MQ=255)                                                                                                                        tcaGGCCGCTATCTAGTTCCTGGCGCTGGGCATCGTCGAGATGCGGCCACACCACTTTACGCGTGCCTGTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATTTGCATCGCAGCCTTCACCGCTTAACTGATCa   >  2:993530/1‑140 (MQ=255)                                                                                                                         caGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACAt  <  1:1010938/140‑1 (MQ=255)                                                                                                                                |                                                                                                                                     ATAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACAT  >  NC_000913/2655927‑2656186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑