Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,860,630	(C)5→4	coding (348/894 nt)	yidL →	putative DNA‑binding transcriptional regulator YidL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,860,626	0	C	.	100.0%	215.1 / NA	50	coding (344/894 nt)	yidL	putative DNA‑binding transcriptional regulator YidL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (26/24);  total (26/24)

CAAGGATGTGGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTACCCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAGCAATAAAAAATAATCTGGCAGTCG  >  NC_000913/3860487‑3860760                                                                                                                                            |                                                                                                                                       cAAGGATGTGGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTAc                                                                                                                                        <  2:751440/140‑1 (MQ=255)    ggATGTGGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTAcccc                                                                                                                                     >  2:689028/1‑140 (MQ=255)    ggATGTGGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTAcccc                                                                                                                                     <  2:800509/140‑1 (MQ=255)       tgtgGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTAccccac                                                                                                                                   <  2:316310/139‑3 (MQ=255)       tgtgGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTAccccac                                                                                                                                   >  1:316310/1‑137 (MQ=255)        gtgGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTAccccacca                                                                                                                                 <  2:131897/140‑5 (MQ=255)         tgGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAg                                                                                                                               >  2:318221/1‑140 (MQ=255)         tgGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAg                                                                                                                               >  2:618954/1‑140 (MQ=255)         tgGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAg                                                                                                                               >  1:837422/1‑140 (MQ=255)             aaaaTTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTatg                                                                                                                           <  2:474654/140‑1 (MQ=255)             aaaaTTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTatg                                                                                                                           <  2:111941/140‑1 (MQ=255)             aaaaTTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTatg                                                                                                                           <  1:689028/140‑1 (MQ=255)                   cTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGat                                                                                                                     <  2:957652/140‑1 (MQ=255)                          aGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCg                                                                                                              <  1:618954/140‑1 (MQ=255)                          aGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCg                                                                                                              <  1:201389/140‑1 (MQ=255)                          aGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCg                                                                                                              <  1:318221/140‑1 (MQ=255)                          aGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCg                                                                                                              <  1:867070/140‑1 (MQ=255)                              tGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTgg                                                                                                          <  1:566292/140‑1 (MQ=255)                               ggCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGt                                                                                                         <  1:512159/140‑1 (MQ=255)                                   aaCAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGc                                                                                                     >  2:975347/1‑140 (MQ=255)                                                gCATGGCGACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGt                                                                                                         >  2:439212/1‑123 (MQ=255)                                                gCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGt                                                                                                         <  1:439212/123‑1 (MQ=255)                                                gCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGttattta                                                                                        >  2:695353/1‑140 (MQ=255)                                                 cATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGt                                                                                                         <  2:91477/122‑1 (MQ=255)                                                 cATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGt                                                                                                         >  1:91477/1‑122 (MQ=255)                                                   tGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATaa                                                                                     >  2:207930/1‑140 (MQ=255)                                                    ggCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCaaa                                                                                                  <  1:864043/126‑1 (MQ=255)                                                    ggCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCaaa                                                                                                  >  2:864043/1‑126 (MQ=255)                                                        aCTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCg                                                                                <  1:695353/140‑1 (MQ=255)                                                         cTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGa                                                                               <  2:16360/140‑1 (MQ=255)                                                               tataTTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTta                                                                         <  1:566196/140‑1 (MQ=255)                                                                    tCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATc                                                                    >  1:904463/1‑140 (MQ=255)                                                                    tCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATc                                                                    >  1:474022/1‑140 (MQ=255)                                                                                tGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCg                                                        >  2:139475/1‑140 (MQ=255)                                                                                tGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCg                                                        >  1:309876/1‑140 (MQ=255)                                                                                tGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCg                                                        <  1:714194/140‑1 (MQ=255)                                                                                      ttCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTg                                                  >  2:284794/1‑140 (MQ=255)                                                                                        cACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCt                                                <  1:139475/140‑1 (MQ=255)                                                                                            ctTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGt                                            >  1:855845/1‑140 (MQ=255)                                                                                             ttATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGtt                                           >  1:215717/1‑140 (MQ=255)                                                                                               aTCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGc                                                 >  1:764241/1‑132 (MQ=255)                                                                                               aTCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGc                                                 <  2:764241/132‑1 (MQ=255)                                                                                                    tgtgAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCt                                                >  1:77542/1‑128 (MQ=255)                                                                                                    tgtgAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCt                                                <  2:77542/128‑1 (MQ=255)                                                                                                         aGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCAcc                               >  1:59651/1‑140 (MQ=255)                                                                                                            tttAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGa                            <  2:837422/140‑1 (MQ=255)                                                                                                              tAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAg                          <  2:474022/140‑1 (MQ=255)                                                                                                              tAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAg                          <  2:855845/140‑1 (MQ=255)                                                                                                              tAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAg                          <  2:904463/140‑1 (MQ=255)                                                                                                              tAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAg                          >  2:933494/1‑140 (MQ=255)                                                                                                                gTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAGCa                        >  1:445620/1‑140 (MQ=255)                                                                                                                           gTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAGCAATAAAAaataa             >  2:231199/1‑140 (MQ=255)                                                                                                                           gTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAGCAATAAAAaataa             >  1:978487/1‑140 (MQ=255)                                                                                                                                      aaTTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAGCAATAAAAAATAATCTGGCAGTCg  >  2:98289/1‑140 (MQ=255)                                                                                                                                      aaTTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAGCAATAAAAAATAATCTGGCAGTCg  >  1:235617/1‑140 (MQ=255)                                                                                                                                      aaTTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAGCAATAAAAAATAATCTGGCAGTCg  >  1:200759/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                       CAAGGATGTGGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTACCCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAGCAATAAAAAATAATCTGGCAGTCG  >  NC_000913/3860487‑3860760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑