Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 379,921 (G)6→7 intergenic (‑40/+148) frmR ← / ← yaiO DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913379,9151.G79.5% 106.0 / 24.2 39intergenic (‑34/+154)frmR/yaiODNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO
Reads supporting (aligned to +/- strand):  ref base . (3/5);  new base G (12/19);  total (15/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.84e-02

GGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATA‑GGGGGG‑TATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAG  >  NC_000913/379776‑380038
                                                                                                                                            |                                                                                                                            
ggCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACtata                                                                                                                               >  1:176269/1‑140 (MQ=255)
ggCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACtata                                                                                                                               >  2:215906/1‑140 (MQ=255)
  cATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATA‑gg                                                                                                                            >  2:38602/1‑140 (MQ=255)
    ttCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATA‑gggg                                                                                                                          <  2:708119/140‑1 (MQ=255)
      cGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATA‑gggggg                                                                                                                        <  2:289464/140‑1 (MQ=255)
         cATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATA‑gggggggta                                                                                                                     <  1:874226/140‑4 (MQ=255)
         cATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATA‑gggggggta                                                                                                                     <  2:716443/140‑4 (MQ=255)
         cATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATA‑gggggggta                                                                                                                     <  1:251157/140‑4 (MQ=255)
         cATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATA‑gggggggta                                                                                                                     <  1:318239/140‑4 (MQ=255)
         cATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATA‑gggggggta                                                                                                                     <  1:551497/140‑4 (MQ=255)
         cATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATA‑gggggggta                                                                                                                     <  1:385892/140‑4 (MQ=255)
            cACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATA‑gggg                                                                                                                          <  2:491647/132‑1 (MQ=255)
            cACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATA‑gggg                                                                                                                          >  1:491647/1‑132 (MQ=255)
              cccTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGG‑TATTCta                                                                                                                <  1:936741/140‑1 (MQ=255)
                      cGACCGTTCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGG‑TATTCTATATGTCaa                                                                                                        >  1:289646/1‑140 (MQ=255)
                        aCCGTTCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGG‑TATTCTATATg                                                                                                            <  2:206862/134‑1 (MQ=255)
                        aCCGTTCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGG‑TATTCTATATg                                                                                                            >  1:206862/1‑134 (MQ=255)
                         ccGTTCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGG‑TATTCTATATGTCAATGc                                                                                                     >  2:876043/1‑140 (MQ=255)
                          cGTTCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGG‑TATTCTATATGTCAATGCa                                                                                                    <  1:215906/140‑1 (MQ=255)
                          cGTTCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGG‑TATTCTATATGTCAATGCa                                                                                                    <  1:206723/140‑1 (MQ=255)
                          cGTTCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGG‑TATTCTATATGTCAATGCa                                                                                                    <  2:176269/140‑1 (MQ=255)
                          cGTTCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATA‑gggggggta                                                                                                                     >  2:849349/1‑120 (MQ=255)
                          cGTTCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATA‑gggggggta                                                                                                                     <  1:849349/123‑4 (MQ=255)
                            ttCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGG‑TATTCTATATGTCa                                                                                                         >  1:731438/1‑133 (MQ=255)
                            ttCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGG‑TATTCTATATGTCa                                                                                                         <  2:731438/133‑1 (MQ=255)
                            ttCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGG‑TATTCTATATGTCAATGCATa                                                                                                  <  2:366133/140‑1 (MQ=255)
                            ttCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGG‑TATTCTATATGTCAATGCATa                                                                                                  <  1:984010/140‑1 (MQ=255)
                             tCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGG‑TATTCTATATGTCAATGCATAc                                                                                                 >  2:977059/1‑140 (MQ=255)
                              ccAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGG‑TATTCTATATGTCAATGCATAcc                                                                                                >  1:384205/1‑140 (MQ=255)
                               cAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGG‑TATTCTATATGTCAATGCATAccc                                                                                               <  1:812297/140‑1 (MQ=255)
                                 gagCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGG‑TATTCTATATGTCAATGCATAccccc                                                                                             <  2:289646/140‑1 (MQ=255)
                                    cATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGG‑TATTCTATATGTCAATGCATACCCCCCta                                                                                          <  1:20312/140‑1 (MQ=255)
                                      tCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGG‑TATTCTATATGTCAATGCATa                                                                                                  >  1:617047/1‑130 (MQ=255)
                                      tCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGG‑TATTCTATATGTCAATGCATa                                                                                                  <  2:617047/130‑1 (MQ=255)
                                       cAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGG‑TATTCTATATGTCAATGCa                                                                                                    >  1:813610/1‑127 (MQ=255)
                                       cAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGG‑TATTCTATATGTCAATGCa                                                                                                    <  2:813610/127‑1 (MQ=255)
                                       cAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGG‑TATTCTATATGTCAATGCATa                                                                                                  <  1:907532/129‑1 (MQ=255)
                                       cAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGG‑TATTCTATATGTCAATGCATa                                                                                                  >  2:907532/1‑129 (MQ=255)
                                       cAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGG‑TATTCTATATGTCAATGCATACCCCCCtata                                                                                        >  1:942771/1‑139 (MQ=255)
                                       cAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGG‑TATTCTATATGTCAATGCATACCCCCCtata                                                                                        <  2:942771/139‑1 (MQ=255)
                                       cAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGG‑TATTCTATATGTCAATGCATACCCCCCta                                                                                          >  2:647823/1‑137 (MQ=255)
                                       cAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGG‑TATTCTATATGTCAATGCATACCCCCCta                                                                                          <  1:647823/137‑1 (MQ=255)
                                       cAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGG‑TATTCTATATGTCAATGCATACCCCCCTATAg                                                                                       >  1:256797/1‑140 (MQ=255)
                                         aTCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGG‑TATTCTATATGTCAATGCATACCCCCCTATAGta                                                                                     <  1:38602/140‑1 (MQ=255)
                                                     aTACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGG‑TATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGga                                                                         <  2:387613/140‑1 (MQ=255)
                                                                      aaGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGG‑TATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCgg                                                        <  2:384205/140‑1 (MQ=255)
                                                                                                      gCATTTCGCACCTCATCATCTGCATGCAATATACTATA‑GGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggg                        >  2:256797‑M2/1‑136 (MQ=255)
                                                                                                                 cTCATCATCTGCATGCAATATACTATA‑GGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggggggggctaatcg             <  2:294555‑M2/140‑16 (MQ=255)
                                                                                                                 cTCATCATCTGCATGCAATATACTATA‑GGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggggggggctaatcg             >  1:294555‑M2/1‑125 (MQ=255)
                                                                                                                    atcatcTGCATGCAATATACTATA‑GGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggggggggctaatcggca          <  1:926083‑M2/140‑19 (MQ=255)
                                                                                                                            catgcaATATACTATA‑GGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggggggggctaatcggcagggaaggc  <  1:776373‑M2/140‑27 (MQ=255)
                                                                                                                            catgcaATATACTATA‑GGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggggggggctaatcggcagggaaggc  <  2:983307‑M2/140‑27 (MQ=255)
                                                                                                                                caATATACTATA‑GGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggggggggctaatcggcagggaagg   <  2:522298‑M2/135‑26 (MQ=255)
                                                                                                                                caATATACTATA‑GGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggggggggctaatcggcagggaagg   >  1:522298‑M2/1‑110 (MQ=255)
                                                                                                                                            |                                                                                                                            
GGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATA‑GGGGGG‑TATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAG  >  NC_000913/379776‑380038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑