Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,395,073	(G)5→6	coding (1008/1548 nt)	nnr →	NAD(P)HX epimerase/NAD(P)HX dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,395,068	1	.	G	100.0%	164.1 / NA	47	W335G (TGG→GGG)	nnr	NAD(P)HX epimerase/NAD(P)HX dehydratase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (25/22);  total (25/22)

GCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCCGCATCCTGGCGA  >  NC_000913/4394948‑4395198                                                                                                                          |                                                                                                                                   gcgcCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGc                                                                                                                  >  2:255149/1‑140 (MQ=255) gcgcCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGc                                                                                                                  >  2:66862/1‑140 (MQ=255)     cgcTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCTGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCaaaa                                                                                                              <  2:596323/140‑1 (MQ=255)       ctgctgACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAg                                                                                                            <  1:66862/140‑1 (MQ=255)        tgctgACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCa                                                                                                                      <  1:254073/129‑1 (MQ=255)        tgctgACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCa                                                                                                                      >  2:254073/1‑129 (MQ=255)                 cACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAAttt                                                                                                  >  1:883205/1‑140 (MQ=255)                    gACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCaaaa                                                                                                              >  2:917621/1‑125 (MQ=255)                    gACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCaaaa                                                                                                              <  1:917621/125‑1 (MQ=255)                    gACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCg                                                                                               <  1:365868/140‑1 (MQ=255)                    gACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCg                                                                                               >  1:745351/1‑140 (MQ=255)                      ccGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCa                                                                                             >  2:765762/1‑140 (MQ=255)                      ccGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCa                                                                                             >  2:874830/1‑140 (MQ=255)                         gAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAAc                                                                                          <  2:883205/140‑1 (MQ=255)                         gAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAAc                                                                                          <  2:476949/140‑1 (MQ=255)                           aTTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCg                                                                                        <  1:510899/140‑1 (MQ=255)                              gATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCg                                                                                               <  2:684859/130‑1 (MQ=255)                              gATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCg                                                                                               >  1:684859/1‑130 (MQ=255)                                    gCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTgg                                                                               >  2:425338/1‑140 (MQ=255)                                      aTGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGa                                                                             >  1:693189/1‑140 (MQ=255)                                            tGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGa                                                                       <  2:929405/140‑1 (MQ=255)                                                gATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCa                                                                   <  1:255149/140‑1 (MQ=255)                                                gATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCa                                                                   >  2:874190/1‑140 (MQ=255)                                                       tctctTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAAcc                                                            <  1:874190/140‑1 (MQ=255)                                                        ctctTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACct                                                           >  2:26814/1‑140 (MQ=255)                                                                gAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaa                                                   >  1:853059/1‑140 (MQ=255)                                                                 aaaGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCACGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaat                                                  >  1:710228/1‑140 (MQ=255)                                                                 aaaGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaat                                                  >  2:852743/1‑140 (MQ=255)                                                                 aaaGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaat                                                  >  1:707817/1‑140 (MQ=255)                                                                 aaaGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaat                                                  <  2:745351/140‑1 (MQ=255)                                                                     ccTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaatcaat                                              <  1:558348/140‑1 (MQ=255)                                                                                    tgtgGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAg                                     >  2:477115/1‑134 (MQ=255)                                                                                    tgtgGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAg                                     <  1:477115/134‑1 (MQ=255)                                                                                    tgtgGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTcac                               >  2:690147/1‑140 (MQ=255)                                                                                       ggtggtgATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAAt                            <  1:765762/140‑1 (MQ=255)                                                                                        gtggtgATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTcaca                              <  2:360915/137‑1 (MQ=255)                                                                                        gtggtgATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTcaca                              >  1:360915/1‑137 (MQ=255)                                                                                          tgtgATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATa                                       >  2:647521/2‑126 (MQ=255)                                                                                          tgtgATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATa                                       <  1:647521/125‑1 (MQ=255)                                                                                              aTTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGa                     >  1:768542/1‑140 (MQ=255)                                                                                                  gTCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCAc                 >  1:504623/1‑140 (MQ=255)                                                                                                   tCCCGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCAcg                >  1:617903/1‑140 (MQ=255)                                                                                                     ccGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCAcgcc              <  1:852743/140‑1 (MQ=255)                                                                                                     ccGGTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCAcgcc              <  2:768542/140‑1 (MQ=255)                                                                                                        gTCTGGGCCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCCGCa           >  1:60117/1‑140 (MQ=255)                                                                                                                 aGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCGCAATCGCGTGATCACGCCGCATCCTGGCGa  <  2:710228/140‑1 (MQ=255)                                                                                                                 aGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCCGCATCCTGGCGa  <  2:707817/140‑1 (MQ=255)                                                                                                                          |                                                                                                                                   GCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCCGCATCCTGGCGA  >  NC_000913/4394948‑4395198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑