Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,138,175	(G)5→6	coding (805/942 nt)	flgJ →	putative peptidoglycan hydrolase FlgJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,138,170	1	.	G	100.0%	318.3 / NA	87	Q267R (CAG→CGG)	flgJ	putative peptidoglycan hydrolase FlgJ
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (42/45);  total (42/45)

ACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACA‑‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATC  >  NC_000913/1138038‑1138304                                                                                                                                      |                                                                                                                                        aCGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACA‑‑ggggggc                                                                                                                                 <  1:940131/140‑3 (MQ=255)  cGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACA‑‑ggggggcg                                                                                                                                >  1:442901/1‑137 (MQ=255)  cGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACA‑‑ggg                                                                                                                                     <  1:514813/135‑1 (MQ=255)  cGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACA‑‑ggg                                                                                                                                     >  2:514813/1‑135 (MQ=255)     cgaagcgaagAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAg                                                                                                                              >  2:670100/1‑140 (MQ=255)       aagcgaagAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGc                                                                                                                            <  2:879702/140‑1 (MQ=255)                  gTAAAAGCGAAGTTTGGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGTCTTTTAACGCGTACCCCGCGCTACGCCCCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGAcg                                                                                                                 <  2:699991/140‑1 (MQ=255)                    aaaaGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGAcgcg                                                                                                               <  2:442901/140‑1 (MQ=255)                      aaGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCggg                                                                                                             <  2:790332/140‑1 (MQ=255)                      aaGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCggg                                                                                                             <  2:941695/140‑1 (MQ=255)                         cGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTa                                                                                                          >  2:430805/1‑140 (MQ=255)                           aaGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTa                                                                                                                        <  1:755937/124‑1 (MQ=255)                           aaGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTa                                                                                                                        >  2:755937/1‑124 (MQ=255)                              tttCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTACCCCGCGCTCCGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCa                                                                                                     <  2:595761/140‑1 (MQ=255)                              tttCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCa                                                                                                     <  2:685418/140‑1 (MQ=255)                               ttCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCAc                                                                                                    <  1:260030/140‑1 (MQ=255)                               ttCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCAc                                                                                                    <  1:213999/140‑1 (MQ=255)                               ttCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCAc                                                                                                    <  1:191419/140‑1 (MQ=255)                                            cTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCAcc                                                                                                   >  2:640449/1‑128 (MQ=255)                                            cTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCAcc                                                                                                   <  1:640449/128‑1 (MQ=255)                                              cGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGc                                                                                     >  2:43136/1‑140 (MQ=255)                                              cGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGc                                                                                     >  2:238092/1‑140 (MQ=255)                                                tATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGccc                                                                                   >  2:604260/1‑140 (MQ=255)                                                 aTCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCg                                                                                  >  2:23542/1‑140 (MQ=255)                                                 aTCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCg                                                                                  >  2:325966/1‑140 (MQ=255)                                                 aTCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCg                                                                                  >  2:730809/1‑140 (MQ=255)                                                 aTCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCg                                                                                  >  2:751387/1‑140 (MQ=255)                                                 aTCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCg                                                                                  >  1:322958/1‑140 (MQ=255)                                                      gAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCa                                                                                <  2:107063/137‑1 (MQ=255)                                                      gAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCa                                                                                >  1:107063/1‑137 (MQ=255)                                                          ccTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCAc                                                                         <  2:322958/140‑1 (MQ=255)                                                             tGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCaa                                                                      >  1:558046/1‑140 (MQ=255)                                                             tGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCaa                                                                      >  1:894507/1‑140 (MQ=255)                                                                cGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACAt                                                                   >  2:316786/1‑140 (MQ=255)                                                                     tACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTc                                                              <  1:316786/140‑1 (MQ=255)                                                                      aCGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTca                                                             <  2:862469/140‑1 (MQ=255)                                                                       cGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTcag                                                            >  2:266377/1‑140 (MQ=255)                                                                        gTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTcagc                                                           <  1:96980/140‑1 (MQ=255)                                                                                 ttAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAAt                                                  >  2:931450/1‑140 (MQ=255)                                                                                 ttAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAAt                                                  >  2:718990/1‑140 (MQ=255)                                                                                 ttAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAAt                                                  >  1:39951/1‑140 (MQ=255)                                                                                   aaCGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCg                                                >  2:844917/1‑140 (MQ=255)                                                                                     cgcgTACCCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGAt                                              <  1:266377/140‑1 (MQ=255)                                                                                     cgcgTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGAt                                              <  1:670100/140‑1 (MQ=255)                                                                                     cgcgTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGAt                                              <  1:635656/140‑1 (MQ=255)                                                                                      gcgTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATa                                             >  2:113487/1‑140 (MQ=255)                                                                                      gcgTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATa                                             >  2:235813/1‑140 (MQ=255)                                                                                        gTAACCCGCGCTATGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAg                                           >  2:723838/1‑140 (MQ=255)                                                                                           aCCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGATGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGa                                        >  1:90008/1‑140 (MQ=255)                                                                                           aCCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGa                                        >  2:983753/1‑140 (MQ=255)                                                                                           aCCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGa                                        >  2:357765/1‑140 (MQ=255)                                                                                           aCCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGa                                        >  2:366523/1‑140 (MQ=255)                                                                                           aCCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGa                                        >  2:170836/1‑140 (MQ=255)                                                                                           aCCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGa                                        >  1:662407/1‑140 (MQ=255)                                                                                            cccGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGATGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGAc                                       <  2:90008/140‑1 (MQ=255)                                                                                               gcgcTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAg                                    >  1:792657/1‑140 (MQ=255)                                                                                                cgcTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGTCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAgg                                   >  1:331183/1‑140 (MQ=255)                                                                                                cgcTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAgg                                   <  1:23542/140‑1 (MQ=255)                                                                                                cgcTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAgg                                   <  1:730809/140‑1 (MQ=255)                                                                                                     cgccgcCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGAt                                              <  2:814638/124‑1 (MQ=255)                                                                                                     cgccgcCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGAt                                              >  1:814638/1‑124 (MQ=255)                                                                                                     cgccgcCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGc                              <  1:170836/140‑1 (MQ=255)                                                                                                     cgccgcCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGc                              <  1:357765/140‑1 (MQ=255)                                                                                                     cgccgcCGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGc                              <  2:558046/140‑1 (MQ=255)                                                                                                      gccgccGTGACGACCGCCGCGAGTGCGGAACAGGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGc                              >  1:472498/1‑140 (MQ=255)                                                                                                      gccgccGTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCa                             >  1:887561/1‑140 (MQ=255)                                                                                                       ccgccgTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGTCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCaa                            <  2:331183/140‑1 (MQ=255)                                                                                                           cgTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAAcc                        <  1:604260/140‑1 (MQ=255)                                                                                                           cgTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAAcc                        >  1:499717/1‑140 (MQ=255)                                                                                                           cgTGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAAcc                        <  2:499717/140‑1 (MQ=255)                                                                                                             tGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTa                      >  1:245696/1‑140 (MQ=255)                                                                                                             tGACGACCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTa                      <  1:983753/140‑1 (MQ=255)                                                                                                              gacgacCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTAc                     <  1:718990/140‑1 (MQ=255)                                                                                                              gacgacCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTAc                     <  1:366523/140‑1 (MQ=255)                                                                                                              gacgacCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTAc                     <  1:844917/140‑1 (MQ=255)                                                                                                              gacgacCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTAc                     <  2:792657/140‑1 (MQ=255)                                                                                                                  acCGCCGCGAGTGCGGAACAGGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGt                  <  2:472498/140‑1 (MQ=255)                                                                                                                  acCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCt                       <  2:154246/134‑1 (MQ=255)                                                                                                                  acCGCCGCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCt                       >  1:154246/1‑134 (MQ=255)                                                                                                                   ccgccgCGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTAt                <  1:723838/140‑1 (MQ=255)                                                                                                                    cgccgcGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATg               <  1:113487/140‑1 (MQ=255)                                                                                                                    cgccgcGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATg               <  2:894507/140‑1 (MQ=255)                                                                                                                    cgccgcGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATg               <  1:235813/140‑1 (MQ=255)                                                                                                                      ccgcGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGaa             <  1:325966/140‑1 (MQ=255)                                                                                                                      ccgcGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGaa             >  2:588742/1‑140 (MQ=255)                                                                                                                      ccgcGAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGaa             >  2:231041/1‑140 (MQ=255)                                                                                                                       cgcgAGTGCGGAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAAc            >  2:449540/1‑140 (MQ=255)                                                                                                                                ggAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAAt   <  1:231041/140‑1 (MQ=255)                                                                                                                                ggAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAAt   <  2:887561/140‑1 (MQ=255)                                                                                                                                ggAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAAt   <  1:931450/140‑1 (MQ=255)                                                                                                                                 gAACAG‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATc  >  1:637584/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                        ACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACA‑‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATC  >  NC_000913/1138038‑1138304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑