Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,514,665	(G)5→6	coding (835/942 nt)	ydcU →	putative ABC transporter membrane subunit YdcU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,514,660	1	.	G	100.0%	112.5 / NA	32	Q277R (CAG→CGG)	ydcU	putative ABC transporter membrane subunit YdcU
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (18/14);  total (18/14)

TATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCACTCACACTGGGCGATTTTATCGTCCCGCAGCTGGTTGGTCCTCCAGGATATTTTATCGGCAATATGGTTTATTCCCAGCA‑‑GGGGGCGATTGGCAATATGCCGATGGCGGCGGCATTCACCCTGGTGCCGATTATTCTCATCGCACTGTACCTGGCGTTCGTGAAACGTCTGGGAGCGTTCGATGCACTCTGAACGCGCACCGTTTTTCCT  >  NC_000913/1514521‑1514790                                                                                                                                             |                                                                                                                                    tATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCACTCACACTGGGCGATTTTATCGTCCCGCAGCTGGTTGGTCCTCCAGGATATTTTATCGGCAATATGGTTTATTCCcagca                                                                                                                                      >  2:977814/1‑140 (MQ=255) tATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCACTCACACTGGGCGATTTTATCGTCCCGCAGCTGGTTGGTCCTCCAGGATATTTTATCGGCAATATGGTTTATTCCcagca                                                                                                                                      >  2:274576/1‑140 (MQ=255)      ggtgCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCACTCACACTGGGCGATTTTATCGTCCCGCAGCTGGTTGGTCCTCCAGGATATTTTATCGGCAATATGGTTTATTCCCAGCA‑‑ggggg                                                                                                                               <  1:583378/140‑1 (MQ=255)         gctgcCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCACTCACACTGGGCGATTTTATCGTCCCGCAGCTGGTTGGTCCTCCAGGATATTTTATCGGCAATATGGTTTATTCCCAGCA‑‑ggggggcg                                                                                                                            <  1:274576/140‑4 (MQ=255)         gctgcCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCACTCACACTGGGCGATTTTATCGTCCCGCAGCTGGTTGGTCCTCCAGGATATTTTATCGGCAATATGGTTTATTCCCAGCA‑‑ggggggcg                                                                                                                            <  1:977814/140‑4 (MQ=255)           tgcCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCACTCACACTGGGCGATTTTATCGTCCCGCAGCTGGTTGGTCCTCCAGGATATTTTATCGGCAATATGGTTTATTCCCAGCA‑‑ggggggcgat                                                                                                                          <  1:963475/140‑6 (MQ=255)             ccGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCACTCACACTGGGCGATTTTATCGTCCCGCAGCTGGTTGGTCCTCCAGGATATTTTATCGGCAATATGGTTTATTCCCAGCAG‑GGGGGCGATTg                                                                                                                         >  2:225259/1‑140 (MQ=255)               gCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCACTCACACTGGGCGATTTTATCGTCCCGCAGCTGGTTGGTCCTCCAGGATATTTTATCGGCAATATGGTTTATTCCCAGCAG‑GGGGGCGATTgg                                                                                                                        <  2:83083/139‑1 (MQ=255)               gCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCACTCACACTGGGCGATTTTATCGTCCCGCAGCTGGTTGGTCCTCCAGGATATTTTATCGGCAATATGGTTTATTCCCAGCAG‑GGGGGCGATTgg                                                                                                                        >  1:83083/1‑139 (MQ=255)                               aTTGCCGCTGGCTCTATCTTTACCTTCTCACTCACACTGGGCGATTTTATCGTCCCGCAGCTGGTTGGTCCTCCAGGATATTTTATCGGCAATATGGTTTATTCCCAGCAG‑GGGGGCGATTGGCAATATGCCGATggcgg                                                                                                       >  1:220876/1‑140 (MQ=255)                                 tGCCGCTGGCTCTATCTTTACCTTCTCACTCACACTGGGCGATTTTATCGTCCCGCAGCTGGTTGGTCCTCCAGGATATTTTATCGGCAATATGGTTTATTCCCAGCAG‑GGGGGCGATTGGCAATATGCCGATggcggcg                                                                                                     <  2:220876/140‑1 (MQ=255)                                      cTGGCTCTATCTTTACCTTCTCACTCACACTGGGCGATTTTATCGTCCCGCAGCTGGTTGGTCCTCCAGGATATTTTATCGGCAATATGGTTTATTCCCAGCAG‑GGGGGCGATTGGCAATATGCCGATGGCGGCGGCAtt                                                                                                <  2:216563/140‑1 (MQ=255)                                        ggCTCTATCTTTACCTTCTCACTCACACTGGGCGATTTTATCGTCCCGCAGCTGGTTGGTCCTCCAGGATATTTTATCGGCAATATGGTTTATTCCCAGCAG‑GGGGGCGATTGGCAATATGCCGATGGCGGCGGCATTCa                                                                                              >  2:282684/1‑140 (MQ=255)                                          ctctATCTTTACCTTCTCACTCACACTGGGCGATTTTATCGTCCCGCAGCTGGTTGGTCCTCCAGGATATTTTATCGGCAATATGGTTTATTCCCAGCAG‑GGGGGCGATTGGCAATATGCCGATGGCGGCGGCATTCAcc                                                                                            <  2:469169/140‑1 (MQ=255)                                           tctATCTTTACCTTCTCACTCACACTGGGCGATTTTATCGTCCCGCAGCTGGTTGGTCCTCCAGGATATTTTATCGGCAATATGGTTTATTCCCAGCAG‑GGGGGCGATTGGCAATATGCCGATGGCGGCGGCATTCAccc                                                                                           <  1:282684/140‑1 (MQ=255)                                                    aCCTTCTCACTCACACTGGGCGATTTTATCGTCCCGCAGCTGGTTGGTCCTCCAGGATATTTTATCGGCAATATGGTTTATTCCCAGCAG‑GGGGGCGATTGGCAATATGCCGATGGCGGCGGCATTCACCCTGGTGCCGa                                                                                  >  2:32262/1‑140 (MQ=255)                                                         ctcactcaCACTGGGCGATTTTATCGTCCCGCAGCTGGTTGGTCCTCCAGGATATTTTATCGGCAATATGGTTTATTCCCAGCAGGGGGGGCGATTGGCAATATGCCGATGGCGGCGGCATTCACCCTGGTGCCGattat                                                                              >  2:530591/1‑140 (MQ=255)                                                         ctcactcaCACTGGGCGATTTTATCGTCCCGCAGCTGGTTGGTCCTCCAGGATATTTTATCGGCAATATGGTTTATTCCCAGCAG‑GGGGGCGATTGGCAATATGCCGATGGCGGCGGCATTCACCCTGGTGCCGattatt                                                                             >  2:445177/1‑140 (MQ=255)                                                         ctcactcaCACTGGGCGATTTTATCGTCCCGCAGCTGGTTGGTCCTCCAGGATATTTTATCGGCAATATGGTTTATTCCCAGCAG‑GGGGGCGATTGGCAATATGCCGATGGCGGCGGCATTCACCCTGGTGCCGattatt                                                                             >  2:342173/1‑140 (MQ=255)                                                           cactcacACTGGGCGATTTTATCGTCCCGCAGCTGGTTGGTCCTCCAGGATATTTTATCGGCAATATGGTTTATTCCCAGCAG‑GGGGGCGATTGGCAATATGCCGATGGCGGCGGCATTCACCCTGGTGCCGATTATtct                                                                           >  2:466843/1‑140 (MQ=255)                                                                   cTGGGCGATTTTATCGTCCCGCAGCTGGTTGGTCCTCCAGGATATTTTATCGGCAATATGGTTTATTCCCAGCAG‑GGGGGCGATTGGCAATATGCCGATGGCGGCGGCATTCACCCTGGTGCCGATTATTCTCATCGCAc                                                                   >  1:410890/1‑140 (MQ=255)                                                                      ggCGATTTTATCGTCCCGCAGCTGGTTGGTCCTCCAGGATATTTTATCGGCAATATGGTTTATTCCCAGCAG‑GGGGGCGATTGGCAATATGCCGATGGCGGCGGCATTCACCCTGGTGCCGATTATTCTCATCGCACTGt                                                                >  1:281531/1‑140 (MQ=255)                                                                      ggCGATTTTATCGTCCCGCAGCTGGTTGGTCCTCCAGGATATTTTATCGGCAATATGGTTTATTCCCAGCAG‑GGGGGCGATTGGCAATATGCCGATGGCGGCGGCATTCACCCTGGTGCCGATTATTCTCATCGCACTGt                                                                >  1:386738/1‑140 (MQ=255)                                                                                     ccGCAGCTGGTTGGTCCTCCAGGATATTTTATCGGCAATATGGTTTATTCCCAGCAG‑GGGGGCGATTGGCAATATGCCGATGGCGGCGGCATTCACCCTGGTGCCGATTATTCTCATCGCACt                                                                  <  1:621545/123‑1 (MQ=255)                                                                                     ccGCAGCTGGTTGGTCCTCCAGGATATTTTATCGGCAATATGGTTTATTCCCAGCAG‑GGGGGCGATTGGCAATATGCCGATGGCGGCGGCATTCACCCTGGTGCCGATTATTCTCATCGCACt                                                                  >  2:621545/1‑123 (MQ=255)                                                                                      cGCAGCTGGTTGGTCCTCCAGGATATTTTATCGGCAATATGGTTTATTCCCAGCAG‑GGGGGCGATTGGCAATATGCCGATGGCGGCGGCATTCACCCTGGTGCCGATTATTCTCATCGCACTGTACCTGGCGTTCGTGaa                                                <  1:32262/140‑1 (MQ=255)                                                                                              gttggtCCTCCAGGATATTTTATCGGCAATATGGTTTATTCCCAGCAG‑GGGGGCGATTGGCAATATGCCGATGGCGGCGGCATTCACCCTGGTGCCGATTATTCTCATCGCACTGTACCTGGCGTTCGTGAAACGTCTgg                                        >  2:871487/1‑140 (MQ=255)                                                                                               ttggtCCTCCAGGATATTTTATCGGCAATATGGTTTATTCCCAGCAG‑GGGGGCGATTGGCAATATGCCGATGGCGGCGGCATTCACCCTGGTGCCGATTATTCTCATCGCACTGTACCTGGCGTTCGTGAAACGTCTggg                                       <  1:342173/140‑1 (MQ=255)                                                                                                tggtCCTCCAGGATATTTTATCGGCAATATGGTTTATTCCCAGCAG‑GGGGGCGATTGGCAATATGCCGATGGCGGCGGCATTCACCCTGGTGCCGATTATTCTCATCGCACTGTACCTGGCGTTCGTGAAACGTCTGGGa                                      <  1:477936/140‑1 (MQ=255)                                                                                                    cctccAGGATATTTTATCGGCAATATGGTTTATTCCCAGCAG‑GGGGGCGATTGGCAATATGCCGATGGCGGCGGCATTCACCCTGGTGCCGATTATTCTCATCGCACTGTACCTGGCGTTCGTGAAACGTCTGGGAGCGt                                  <  1:445177/140‑1 (MQ=255)                                                                                                            atatTTTATCGGCAATATGGTTTATTCCCAGCAG‑GGGGGCGATTGGCAATATGCCGATGGCGGCGGCATTCACCCTGGTGCCGATTATTCTCATCGCACTGTACCTGGCGTTCGTGAAACGTCTGGGAGCGTTCGATGCa                          <  2:386738/140‑1 (MQ=255)                                                                                                                tttATCGGCAATATGGTTTATTCCCAGCAG‑GGGGGCGATTGGCAATATGCCGATGGCGGCGGCATTCACCCTGGTGCCGATTATTCTCATCGCACTGTACCTGGCGTTCGTGAAACGTCTGGGAGCGTTCGATGCActct                      >  2:397665/1‑140 (MQ=255)                                                                                                                 ttATCGGCAATATGGTTTATTCCCAGCAG‑GGGGGCGATTGGCAATATGCCGATGGCGGCGGCATTCACCCTGGTGCCGATTATTCTCATCGCACTGTACCTGGCGTTCGTGAAACGTCTGGGAGCGTTCGATGCACTCTg                     <  1:871487/140‑1 (MQ=255)                                                                                                                     cGGCAATATGGTTTATTCCCAGCAG‑GGGGGCGATTGGCAATATGCCGATGGCGGCGGCATTCACCCTGGTGCCGATTATTCTCATCGCACTGTACCTGGCGTTCGTGAAACGTCTGGGAGCGTTCGATGCACTCTGAAcg                 >  1:89963/1‑140 (MQ=255)                                                                                                                          atatGGTTTATTCCCAGCAG‑GGGGGCGATTGGCAATATGCCGATGGCGGCGGCATTCACCCTGGTGCCGATTATTCTCATCGCACTGTACCTGGCGTTCGTGAAACGTCTGGGAGCGTTCGATGCACTCTGAACGCGCAc            >  1:654192/1‑140 (MQ=255)                                                                                                                              ggTTTATTCCCAGCAG‑GGGGGCGATTGGCAATATGCCGATGGCGGCGGCATTCACCCTGGTGCCGATTATTCTCATCGCACTGTACCTGGCGTTCGTGAAACGTCTGGGAGCGTTCGATGCACTCTGAACGCGCACCGtt        <  1:225259/140‑1 (MQ=255)                                                                                                                                    ttCCCAGCAG‑GGGGGCGATTGGCAATATGCCGATGGCGGCGGCATTCACCCTGGTGCCGATTATTCTCATCGCACTGTACCTGGCGTTCGTGAAACGTCTGGGAGCGTTCGATGCACTCTGAACGCGCACCGTTTTTCCt  <  2:654192/140‑1 (MQ=255)                                                                                                                                    ttCCCAGCAG‑GGGGGCGATTGGCAATATGCCGATGGCGGCGGCATTCACCCTGGTGCCGATTATTCTCATCGCACTGTACCTGGCGTTCGTGAAACGTCTGGGAGCGTTCGATGCACTCTGAACGCGCACCGTTTTTCCt  >  2:554784/1‑140 (MQ=255)                                                                                                                                             |                                                                                                                                    TATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCACTCACACTGGGCGATTTTATCGTCCCGCAGCTGGTTGGTCCTCCAGGATATTTTATCGGCAATATGGTTTATTCCCAGCA‑‑GGGGGCGATTGGCAATATGCCGATGGCGGCGGCATTCACCCTGGTGCCGATTATTCTCATCGCACTGTACCTGGCGTTCGTGAAACGTCTGGGAGCGTTCGATGCACTCTGAACGCGCACCGTTTTTCCT  >  NC_000913/1514521‑1514790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑