Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,550,453	(G)5→6	intergenic (+5/‑8)	fdnG → / → fdnH	formate dehydrogenase N subunit alpha/formate dehydrogenase N subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,550,448	1	.	G	100.0%	151.4 / NA	42	*1016* (TAA→TAG)	fdnG	formate dehydrogenase N subunit alpha
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (22/20);  total (22/20)

TGGGTATTCCAATCCACTGGGGCTTTGAGGGTGTCGCGCGTAAAGGTTATATCGCTAACACTCTGACGCCGAATGTCGGTGATGCAAACTCGCAAACGCCGGAATATAAAGCGTTCTTAGTCAACATCGAGAAGGCGTAA‑GGGGGCGAACAGATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGCAACTAACTCCATCACGCCGCCTTCTCAGGTGCGTGATTACAAAGCAGAAGTCGCAAAACTTATCGACGTTTCCACCT  >  NC_000913/1550309‑1550575                                                                                                                                             |                                                                                                                                tGGGTATTCCAATCCACTGGGGCTTTGAGGGTGTCGCGCGTAAAGGTTATATCGCTAACACTCTGACGCCGAATGTCGGTGATGCAAACTCGCAAACGCCGGAATATAAAGCGTTCTTAGTCAACATCGAGAAGGCGTaa                                                                                                                                  >  2:932917/1‑140 (MQ=255) tGGGTATTCCAATCCACTGGGGCTTTGAGGGTGTCGCGCGTAAAGGTTATATCGCTAACACTCTGACGCCGAATGTCGGTGATGCAAACTCGCAAACGCCGGAATATAAAGCGTTCTTAGTCAACATCGAGAAGGCGTaa                                                                                                                                  <  1:506659/140‑1 (MQ=255)   ggTATTCCAATCCACTGGGGCTTTGAGGGTGTCGCGCGTAAAGGTTATATCGCTAACACTCTGACGCCGAATGTCGGTGATGCAAACTCGCAAACGCCGGAATATAAAGCGTTCTTAGTCAACATCGAGAAGGCGTaa                                                                                                                                  <  2:624383/138‑1 (MQ=255)   ggTATTCCAATCCACTGGGGCTTTGAGGGTGTCGCGCGTAAAGGTTATATCGCTAACACTCTGACGCCGAATGTCGGTGATGCAAACTCGCAAACGCCGGAATATAAAGCGTTCTTAGTCAACATCGAGAAGGCGTaa                                                                                                                                  >  1:624383/1‑138 (MQ=255)      aTTCCAATCCACTGGGGCTTTGAGGGTGTCGCGCGTAAAGGTTATATCGCTAACACTCTGACGCCGAATGTCGGTGATGCAAACTCGCAAACGCCGGAATATAAAGCGTTCTTAGTCAACATCGAGAAGGCGTAA‑ggggg                                                                                                                            >  2:211912/1‑140 (MQ=255)         ccAATCCACTGGGGCTTTGAGGGTGTCGCGCGTAAAGGTTATATCGCTAACACTCTGACGCCGAATGTCGGTGATGCAAACTCGCAAACGCCGGAATATAAAGCGTTCTTAGTCAACATCGAGAAGGCGTAA‑ggggggcg                                                                                                                         <  1:932917/140‑4 (MQ=255)             tCCACTGGGGCTTTGAGGGTGTCGCGCGTAAAGGTTATATCGCTAACACTCTGACGCCGAATGTCGGTGATGCAAACTCGCAAACGCCGGAATATAAAGCGTTCTTAGTCAACATCGAGAAGGCGTAAGGGGGGCGAACa                                                                                                                      <  1:820623/140‑1 (MQ=255)                 cTGGGGCTTTGAGGGTGTCGCGCGTAAAGGTTATATCGCTAACACTCTGACGCCGAATGTCGGTGATGCAAACTCGCAAACGCCGGAATATAAAGCGTTCTTAGTCAACATCGAGAAGGCGTAAGGGGGGCGAACAGATg                                                                                                                  >  2:547171/1‑140 (MQ=255)                  tGGGGCTTTGAGGGTGTCGCGCGTAAAGGTTATATCGCTAACACTCTGACGCCGAATGTCGGTGATGCAAACTCACAAACGCCGGAATATAAAGCGTTCTTAGTCAACATCGAGAAGGCGTAAGGGGGGCGAACAGATgg                                                                                                                 <  1:314491/140‑1 (MQ=255)                     ggCTTTGAGGGTGTCGCGCGTAAAGGTTATATCGCTAACACTCTGACGCCGAATGTCGGTGATGCAAACTCGCAAACGCCGGAATATAAAGCGTTCTTAGTCAACATCGAGAAGGCGTAAGGGGGGCGAACAGATGGCTa                                                                                                              <  1:211912/140‑1 (MQ=255)                         ttGAGGGTGTCGCGCGTAAAGGTTATATCGCTAACACTCTGACGCCGAATGTCGGTGATGCAAACTCGCAAACGCCGGAATATAAAGCGTTCTTAGTCAACATCGAGAAGGCGTAAGGGGGGCGAACAGATGGCTATGGa                                                                                                          >  1:73587/1‑140 (MQ=255)                                tgtCGCGCGTAAAGGTTATATCGCTAACACTCTGACGCCGAATGTCGGTGATGCAAACTCGCAAACGCCGGAATATAAAGCGTTCTTAGTCAACATCGAGAAGGCGTAAGGGGGGCGAACAGATGGCTATGGAAACGCAg                                                                                                   >  1:578500/1‑140 (MQ=255)                                tgtCGCGCGTAAAGGTTATATCGCTAACACTCTGACGCCGAATGTCGGTGATGCAAACTCGCAAACGCCGGAATATAAAGCGTTCTTAGTCAACATCGAGAAGGCGTAAGGGGGGCGAACAGATGGCTATGGAAACGCAg                                                                                                   >  1:638908/1‑140 (MQ=255)                                 gtCGCGCGTAAAGGTTATATCGCTAACACTCTGACGCCGAATGTCGGTGATGCAAACTCGCAAACGCCGGAATATAAAGCGTTCTTAGTCAACATCGAGAAGGCGTAAGGGGGGCGAACAGATGGCTATGGAAACGCAgg                                                                                                  <  2:411174/140‑1 (MQ=255)                                  tCGCGCGTAAAGGTTATATCGCTAACACTCTGACGCCGAATGTCGGTGATGCAAACTCGCAAACGCCGGAATATAAAGCGTTCTTAGTCAACATCGAGAAGGCGTAAGGGGGGCGAACAGATGGCTATGGAAACGCAGGa                                                                                                 <  2:73587/140‑1 (MQ=255)                                  tCGCGCGTAAAGGTTATATCGCTAACACTCTGACGCCGAATGTCGGTGATGCAAACTCGCAAACGCCGGAATATAAAGCGTTCTTAGTCAACATCGAGAAGGCGTAAGGGGGGCGAACAGATGGCTATGGAAACGCAGGa                                                                                                 <  2:578500/140‑1 (MQ=255)                                  tCGCGCGTAAAGGTTATATCGCTAACACTCTGACGCCGAATGTCGGTGATGCAAACTCGCAAACGCCGGAATATAAAGCGTTCTTAGTCAACATCGAGAAGGCGTAAGGGGGGCGAACAGATGGCTATGGAAACGCAGGa                                                                                                 <  2:638908/140‑1 (MQ=255)                                    gcgcgTAAAGGTTATATCGCTAACACTCTGACGCCGAATGTCGGTGATGCAAACTCGCAAACGCCGGAATATAAAGCGTTCTTAGTCAACATCGAGAAGGCGTAAGGGGGGCGAACAGATGGCTATGGAAACGCAGGACa                                                                                               <  2:751971/140‑1 (MQ=255)                                    gcgcgTAAAGGTTATATCGCTAACACTCTGACGCCGAATGTCGGTGATGCAAACTCGCAAACGCCGGAATATAAAGCGTTCTTAGTCAACATCGAGAAGGCGTAAGGGGGGCGAACAGATGGCTATGGAAACGCAGGACa                                                                                               <  2:667888/140‑1 (MQ=255)                                      gcgTAAAGGTTATATCGCTAACACTCTGACGCCGAATGTCGGTGATGCAAACTCGCAAACGCCGGAATATAAAGCGTTCTTAGTCAACATCGAGAAGGCGTAAGGGGGGCGAACAGATGGCTATGGAAACGCAGGACAtt                                                                                             >  2:96810/1‑140 (MQ=255)                                            aGGTTATATCGCTAACACTCTGACGCCGAATGTCGGTGATGCAAACTCGCAAACGCCGGAATATAAAGCGTTCTTAGTCAACATCGAGAAGGCGTAAGGGGGGCGAACAGATGGCTATGGAAACGCAGGACATTATCaaa                                                                                       <  2:719048/140‑1 (MQ=255)                                                  tatCGCTAACACTCTGACGCCGAATGTCGGTGATGCAAACTCGCAAACGCCGGAATATAAAGCGTTCTTAGTCAACATCGAGAAGGCGTAAGGGGGGCGAACAGATGGCTATGGAAACGCAGGACATTATCAAAAGGTcc                                                                                 <  1:96810/140‑1 (MQ=255)                                                  tatCGCTAACACTCTGACGCCGAATGTCGGTGATGCAAACTCGCAAACGCCGGAATATAAAGCGTTCTTAGTCAACATCGAGAAGGCGTAAGGGGGGCGAACAGATGGCTATGGAAACGCAGGACATTATCAAAAGGTcc                                                                                 >  1:20988/1‑140 (MQ=255)                                                        tAACACTCTGACGCCGAATGTCGGTGATGCAAACTCGCAAACGCCGGAATATAAAGCGTTCTTAGTCAACATCGAGAAGGCGTAAGGGGGGCGAACAGATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGCaact                                                                           >  1:451243/1‑140 (MQ=255)                                                               ctGACGCCGAATGTCGGTGATGCAAACTCGCAAACGCCGGAATATAAAGCGTTCTTAGTCAACATCGAGAAGGCGTAAGGGGGGCGAACAGATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGCAACTAACTCCa                                                                    >  1:655973/1‑140 (MQ=255)                                                                tGACGCCGAATGTCGGTGATGCAAACTCGCAAACGCCGGAATATAAAGCGTTCTTAGTCAACATCGAGAAGGCGTAAGGGGGGCGAACAGATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGCAACTAACTCCAt                                                                   >  1:980067/1‑140 (MQ=255)                                                                tGACGCCGAATGTCGGTGATGCAAACTCGCAAACGCCGGAATATAAAGCGTTCTTAGTCAACATCGAGAAGGCGTAAGGGGGGCGAACAGATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGCAACTAACTCCAt                                                                   >  1:318559/1‑140 (MQ=255)                                                                  aCGCCGAATGTCGGTGATGCAAACTCGCAAACGCCGGAATATAAAGCGTTCTTAGTCAACATCGAGAAGGCGTAAGGGGGGCGAACAGATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGCAACTAACTCCATCa                                                                 >  2:365201/1‑140 (MQ=255)                                                                      cGAATGTCGGTGATGCAAACTCGCAAACGCCGGAATATAAAGCGTTCTTAGTCAACATCGAGAAGGCGTAAGGGGGGCGAACAGATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGCAACTAACTCCATCAcgcc                                                             <  2:451243/140‑1 (MQ=255)                                                                        aaTGTCGGTGATGCAAACTCGCAAACGCCGGAATATAAAGCGTTCTTAGTCAACATCGAGAAGGCGTAAGGGGGGCGAACAGATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGCAACTAACTCCATCAcgccgc                                                           >  1:599741/1‑140 (MQ=255)                                                                        aaTGTCGGTGATGCAAACTCGCAAACGCCGGAATATAAAGCGTTCTTAGTCAACATCGAGAAGGCGTAAGGGGGGCGAACAGATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGCAACTAACTCCATCAcgcc                                                             <  1:753715/138‑1 (MQ=255)                                                                        aaTGTCGGTGATGCAAACTCGCAAACGCCGGAATATAAAGCGTTCTTAGTCAACATCGAGAAGGCGTAAGGGGGGCGAACAGATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGCAACTAACTCCATCAcgcc                                                             >  2:753715/1‑138 (MQ=255)                                                                                           cGCAAACGCCGGAATATAAAGCGTTCTTAGTCAACATCGAGAAGGCGTAAGGGGGGCGAACAGATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGCAACTAACTCCATCACGCCGCCTTCTCAGGTGCGTGATTa                                        <  2:655973/140‑1 (MQ=255)                                                                                                    cGGAATATAAAGCGTTCTTAGTCAACATCGAGAAGGCGTAAGGGGGGCGAACAGATGGCCATGGAAACGCAGGACATTATCAAAAGGTCCGCAACTAACTCCATCACGCCGCCTTCTCAGGTGCGTGATTACAAAGCAGa                               >  2:140438/1‑140 (MQ=255)                                                                                                            aaaGCGTTCTTAGTCAACATCGAGAAGGCGTAAGGGGGGCGAACAGATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGCAACTAACTCCATCACGCCGCCTTCTCAGGTGCGTGATTACAAAGCAGAAGTCGCaa                       >  2:400574/1‑140 (MQ=255)                                                                                                            aaaGCGTTCTTAGTCAACATCGAGAAGGCGTAAGGGGGGCGAACAGATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGCAACTAACTCCATCACGCCGCCTTCTCAGGTGCGTGATTACAAAGCAGAAGTCGCaa                       <  2:599741/140‑1 (MQ=255)                                                                                                            aaaGCGTTCTTAGTCAACATCGAGAAGGCGTAAGGGGGGCGAACAGATGGCTAAGGAAACGCAGGACATTATCAAAAGGTCCGCAACTAACTCCATCACGCCGCCTTCTGAGGGGCGTGATTACAAAGCAGAAGTCGCaa                       >  2:246020/1‑140 (MQ=255)                                                                                                                cGTTCTTAGTCAACATCGAGAAGGCGTAAGGGGGGCGAACAGATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGCAACTAACTCCATCACGCCGCCTTCTCAGGTGCGTGATTACAAAGCAGAAGTCGCAAAACt                   >  1:100407/1‑140 (MQ=255)                                                                                                                   tCTTAGTCAACATCGAGAAGGCATAAGGGGGGCGAACAGATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGCAACTAACTCCATCACGCCGCCTTCTCAGGTGTGTGATTACAAAGCAGAAGTCGCAAAACTTAt                >  1:254195/1‑140 (MQ=255)                                                                                                                    cTTAGTCAACATCGAGAAGGCATAAGGGGGGCGAACAGATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGCAACTAACTCCATCACGCCGCCTTCTCAGGTGTGTGATTACAAAGCAGAAGTCGCAAAACTTATc               <  2:254195/140‑1 (MQ=255)                                                                                                                     ttAGTCAACATCGAGAAGGCGTAAGGGGGGCGAACAGATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGCAACTAACTCCATCACGCCGCCTTCTCAGGTGCGTGATTACAAAGCAGAAGTCGCAAAACTTATCg              <  2:318559/140‑1 (MQ=255)                                                                                                                     ttAGTCAACATCGAGAAGGCGTAAGGGGGGCGAACAGATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGCAACTAACTCCATCACGCCGCCTTCTCAGGTGCGTGATTACAAAGCAGAAGTCGCAAAACTTATCg              <  2:952538/140‑1 (MQ=255)                                                                                                                       aGTCAACATCGAGAAGGCGTAAGGGGGGCGAACAGATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGCAACTAACTCCATCACGCCGCCTTCTCAGGTGCGTGATTACAAAGCAGAAGTCGCAAAACTTATCGAc            >  2:751763/1‑140 (MQ=255)                                                                                                                          cAACATCGAGAAGGCGTAAGGGGGGCGAACAGATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGCAACTAACTCCATCACGCCGCCTTCTCAGGTGCGTGATTACAAAGCAGAAGTCGCAAAACTTATCGACGtt         <  2:100407/140‑1 (MQ=255)                                                                                                                          cAACATCGAGAAGGCGTAAGGGGGGCGAACAGATGGCCATGGAAACGCAGGACATTATCAAAAGGTCCGCAACTAACTCCATCACGCCGCCTTCTCAGGTGCGTGATTACAAAGCAGAAGTCGCAAAACTTATCGACGtt         <  1:140438/140‑1 (MQ=255)                                                                                                                               tCGAGAAGGCGTAAGGGGGGCGAACAGATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGCAACTAACTCCATCACGCCGCCTTCTCAGGTGCGTGATTACAAAGCAGAAGTCGCAAAACTTATCGACGTTTCCAc    >  2:411334/1‑140 (MQ=255)                                                                                                                                cGAGAAGGCGTAAGGGGGGCGAACAGATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGCAACTAACTCCATCACGCCGCCTTCTCAGGTGCGTGATTACAAAGCAGAAGTCGCAAAACTTATCGACGTTTCCAcc   >  2:900423/1‑140 (MQ=255)                                                                                                                                 gagaAGGCGTAAGGGGGGCGAACAGATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGCAACTAACTCCATCACGCCGCCTTCTCAGGTGCGTGATTACAAAGCAGAAGTCGCAAAACTTATCGACGTTTCCACCt  >  1:113434/1‑140 (MQ=255)                                                                                                                                             |                                                                                                                                TGGGTATTCCAATCCACTGGGGCTTTGAGGGTGTCGCGCGTAAAGGTTATATCGCTAACACTCTGACGCCGAATGTCGGTGATGCAAACTCGCAAACGCCGGAATATAAAGCGTTCTTAGTCAACATCGAGAAGGCGTAA‑GGGGGCGAACAGATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGCAACTAACTCCATCACGCCGCCTTCTCAGGTGCGTGATTACAAAGCAGAAGTCGCAAAACTTATCGACGTTTCCACCT  >  NC_000913/1550309‑1550575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑