Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,185,875	(G)7→8	intergenic (‑79/‑42)	rcnR ← / → rcnA	DNA‑binding transcriptional repressor RcnR/Ni(2(+))/Co(2(+)) exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,185,868	1	.	G	100.0%	140.3 / NA	41	intergenic (‑72/‑49)	rcnR/rcnA	DNA‑binding transcriptional repressor RcnR/Ni(2(+))/Co(2(+)) exporter
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (27/14);  total (27/14)

GACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACT‑GGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGC  >  NC_000913/2185731‑2186000                                                                                                                                           |                                                                                                                                        gACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACT‑gg                                                                                                                                       <  1:190640/140‑1 (MQ=255)    cTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACT‑ggggg                                                                                                                                    <  1:779026/140‑1 (MQ=255)       gCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACT‑gggggg‑gg                                                                                                                                >  1:591787/1‑139 (MQ=255)            gAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGG‑G‑A‑GTAg                                                                                                                          >  1:274911/1‑140 (MQ=255)             aaTCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGG‑G‑A‑GTAGa                                                                                                                         >  2:338058/1‑140 (MQ=255)                   aCTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGa                                                                                                                   <  2:274911/140‑1 (MQ=255)                       gCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGc                                                                                                               >  1:112140/1‑140 (MQ=255)                         aCGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCg                                                                                                             >  1:733597/1‑140 (MQ=255)                            cgcTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAAt                                                                                                          <  2:556203/140‑1 (MQ=255)                                     tttCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTAACAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTaa                                                                                                 >  1:606342/1‑140 (MQ=255)                                         tGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAat                                                                                             >  1:577293/1‑140 (MQ=255)                                               tCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATtattat                                                                                       >  1:982501/1‑140 (MQ=255)                                                     aTTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGAc                                                                                 >  1:759918/1‑140 (MQ=255)                                                      ttGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGAcc                                                                                <  1:338058/140‑1 (MQ=255)                                                         tATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGaa                                                                             >  2:568970/1‑140 (MQ=255)                                                                 aTGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAAc                                                                     >  1:433802/1‑140 (MQ=255)                                                                 aTGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAAc                                                                     >  1:323700/1‑140 (MQ=255)                                                                   ggCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAActc                                                                   >  1:898732/1‑140 (MQ=255)                                                                   ggCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAActc                                                                   >  1:691315/1‑140 (MQ=255)                                                                   ggCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAActc                                                                   >  1:521812/1‑140 (MQ=255)                                                                   ggCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAActc                                                                   >  1:259702/1‑140 (MQ=255)                                                                   ggCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAActc                                                                   >  1:412978/1‑140 (MQ=255)                                                                        cacCTGGTTAACAAGAATATGAAAAATCATAGCTCTATTAATCTACTGGGGGATAGTATCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTcttctt                                                              <  2:22620/140‑1 (MQ=255)                                                                        cacCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTcttctt                                                              >  1:731463/1‑140 (MQ=255)                                                                        cacCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGAATCAGGTACTGGGGGGG‑G‑C‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTcttctt                                                              >  2:583358/1‑140 (MQ=255)                                                                         acCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAActcctgtct                                                             >  1:252908/1‑134 (MQ=255)                                                                                  aCAAGAATATGAAAAATCATCGCACCCATGAACTCCTGGGGGGTAGAATCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGaa                                                    <  2:982501/140‑1 (MQ=255)                                                                                         tatGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTg                                             >  1:406496/1‑140 (MQ=255)                                                                                             aaaaaTCATAGCACTATTAATCTACTGGGGGGAAGTATCGGGAACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGATTTTACACCTCTTCTTCAGCAAGAAAACGccggtttt                                         >  2:170175/1‑134 (MQ=255)                                                                                               aaaTCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGttctt                                       <  2:898732/140‑1 (MQ=255)                                                                                                     tAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATccc                                 <  2:406496/140‑1 (MQ=255)                                                                                                        cACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAg                              <  2:591787/140‑1 (MQ=255)                                                                                                         aCTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAgc                             <  2:691315/140‑1 (MQ=255)                                                                                                          tcaTTAATCTGATGGTGGATAGCATCGAGTACG‑GGGGGGCG‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAgcg                            <  2:259702/138‑1 (MQ=255)                                                                                                          cTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAgcg                            <  2:112140/140‑1 (MQ=255)                                                                                                          cTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAgcg                            <  2:412978/140‑1 (MQ=255)                                                                                                                  cTACTGGGGGGTAGTATCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAgcg                            <  1:520346/132‑1 (MQ=255)                                                                                                                  cTACTGGGGGGTAGTATCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAgcg                            >  2:520346/1‑132 (MQ=255)                                                                                                                  cTACTGGGGGGTAGTATCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTa                    >  1:812581/1‑140 (MQ=255)                                                                                                                    gttGGGGGGTAGTATGCGGTGCT‑GGGGGG‑GCA‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACt                  <  2:577293/138‑1 (MQ=255)                                                                                                                     cTGGGGGGTAGTATCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATccc                                 <  2:727732/124‑1 (MQ=255)                                                                                                                     cTGGGGGGTAGTATCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATccc                                 >  1:727732/1‑124 (MQ=255)                                                                                                                     cTGGGGGGTAGTATCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCtt                     >  2:381375/1‑136 (MQ=255)                                                                                                                     cTGGGGGGTAGTATCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCtt                     <  1:381375/136‑1 (MQ=255)                                                                                                                     cTGGGGGGTAGTATCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACtt                 >  2:312100/1‑140 (MQ=255)                                                                                                                         ggggTTCTATCGGTTTCT‑GGGGGGAG‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTg             <  2:521812/140‑1 (MQ=255)                                                                                                                           ggTAGTATCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTgcg           >  1:804742/1‑140 (MQ=255)                                                                                                                             tagtaTCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCt         >  2:192866/1‑140 (MQ=255)                                                                                                                               gtaTCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTc       >  2:147782/1‑140 (MQ=255)                                                                                                                                taTCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCa      <  1:192866/140‑1 (MQ=255)                                                                                                                                 aTCAGGTACTGGGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCAt     <  1:312100/140‑1 (MQ=255)                                                                                                                                    gggTACT‑GGGGGG‑G‑AGGTAGAACCAGATGGCCAAATTAATACTAAGAATAATTATCAGGCCCGAATTTCCACCCCTTCTCCACCAAGGAACCCCTGGGTTCTTCACCCCCACCCCCATCTTCCTTGGTGCGCTTCATGGc  <  2:759918/139‑1 (MQ=255)                                                                                                                                           |                                                                                                                                        GACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACT‑GGGGGG‑G‑A‑GTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGC  >  NC_000913/2185731‑2186000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑