Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	4,157,959	Δ103 bp	coding (1110‑1212/1374 nt)	argH →	argininosuccinate lyase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 4157958	4 (0.070)	46 (0.810)	37/272	0.4	93.9%	coding (1109/1374 nt)	argH	argininosuccinate lyase
	?	NC_000913	4158062 =	2 (0.040)					coding (1213/1374 nt)	argH	argininosuccinate lyase

CAAAGAAGGTCTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4157821‑4157958 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGCAGGCGA  >  NC_000913/4158062‑4158194                                                                                                                                                                                                                                                                                 CAAAGAAGGTCTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTCT                                                                                                                                     <  1:920379/140‑1    AGAAGGTCTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTCTGCC                                                                                                                                  <  2:95554/140‑1            TGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTCTGCCGCTCAGTG                                                                                                                          >  2:755272/1‑140              TTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTCTGCCGCTCAGTGAG                                                                                                                        >  2:371912/1‑140                   CGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTCTGCCGCTCAGTGAGTTGCA                                                                                                                   <  1:710531/140‑1                                  GCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGT                                                                                                    >  2:450886/1‑140                                     tgtcttCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGAT                                                                                                 <  1:965899/134‑1                                      GACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTG                                                                                                   >  2:642367/1‑137                                      GACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTG                                                                                                   <  1:642367/137‑1                                           CCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGA                                                                                           >  1:89444/1‑140                                           CCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGA                                                                                           >  1:730544/1‑140                                             TGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAG                                                                                         <  2:58551/140‑1                                             TGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAG                                                                                         >  1:590225/1‑140                                                 TATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGT                                                                                     >  2:443739/1‑140                                                      CGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATC                                                                                >  2:700804/1‑140                                                         CGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGA                                                                             >  1:896224/1‑140                                                             GGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCT                                                                         <  1:373663/140‑1                                                              GTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTG                                                                        >  1:904322/1‑140                                                                   GGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCT                                                                   >  2:564024/1‑140                                                                      CGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCA                                                                >  2:25828/1‑140                                                                        GCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAAT                                                              >  2:65536/1‑140                                                                             CAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGC                                                         <  2:904322/140‑1                                                                              AGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCC                                                        >  1:274772/1‑140                                                                                   AAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGC                                                         >  1:242502/1‑134                                                                                   AAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGC                                                         <  2:242502/134‑1                                                                                     ACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAA                                                 <  1:443739/140‑1                                                                                               TGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCA                                       <  1:700804/140‑1                                                                                               TGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTG                                            <  1:394845/135‑1                                                                                               TGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGAGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTG                                            >  2:394845/1‑135                                                                                                  CAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAA                                                 <  2:930758/127‑1                                                                                                  CAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAA                                                 >  1:930758/1‑127                                                                                                         CGGCTCAGCAGGGTTACGCCAACGCCACCGAACTTTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCG                             >  2:556447/1‑140                                                                                                           GCTCAGCAGGGTTACGCCAACGCCACCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTC                           >  1:644672/1‑140                                                                                                           GCTCAGCAGGGTTACGCCAACGCCACCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTC                           >  1:88349/1‑140                                                                                                           GCTCAGCAGGGTTACGCCAACGCCACCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTC                           >  1:512009/1‑140                                                                                                              CAGCAGGGTTACGCCAACGCCACCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCA                        <  1:450886/140‑1                                                                                                                      TTACGCCAACGCCACCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCA                >  1:585300/1‑140                                                                                                                      TTACGCCAACGCCACCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCA                >  1:166695/1‑140                                                                                                                        ACGCCAACGCCACCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGG              <  2:89444/140‑1                                                                                                                              ACGCCACCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGC        >  2:246059/1‑140                                                                                                                              ACGCCACCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGC        >  1:370002/1‑140                                                                                                                              ACGCCACCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGC        <  2:166695/140‑1                                                                                                                               CGCCACCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGCA       >  1:378197/1‑140                                                                                                                               CGCCACCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGCA       >  1:660985/1‑140                                                                                                                                    CCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGCAGGCGA  >  1:753824/1‑140                                                                                                                                    CCGAACTCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGCAGGCGA  >  1:886187/1‑140                                                                                                                                                                                                                                                                                 CAAAGAAGGTCTGTTCGACGCGCTCGATACCTGGCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4157821‑4157958 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATTCTGTCGCTGCAATCGTGCCTCGACAAGCGTGCGGCAAAAGGCGGCGTCTCACCGCAGCAGGTGGCGCAGGCGA  >  NC_000913/4158062‑4158194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.