Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	336,000	G→A	A26T (GCG→ACG)	yahE →	DUF2877 domain‑containing protein YahE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	336,000	0	G	A	100.0%	263.1 / NA	75	A26T (GCG→ACG)	yahE	DUF2877 domain‑containing protein YahE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (41/34);  total (41/34)

CCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGCACTCACTCACTTTGATGATCTGTTCCGGCATGGTGATAAGGTTC  >  NC_000913/335872‑336132                                                                                                                                 |                                                                                                                                     ccGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATc                                                                                                                           >  2:159112/1‑140 (MQ=255) ccGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATc                                                                                                                           >  1:713283/1‑140 (MQ=255)  cGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCg                                                                                                                          <  2:358492/140‑1 (MQ=255)  cGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCg                                                                                                                          <  2:713283/140‑1 (MQ=255)                   ggAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCgg                                                                                                                 >  2:154959/1‑132 (MQ=255)                   ggAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCgg                                                                                                                 <  1:154959/132‑1 (MQ=255)                   ggAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCcag                                                                                                         >  2:322272/1‑140 (MQ=255)                   ggAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCcag                                                                                                         >  2:736440/1‑140 (MQ=255)                   ggAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCcag                                                                                                         <  1:607820/140‑1 (MQ=255)                   ggAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCcag                                                                                                         <  1:159112/140‑1 (MQ=255)                    gAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCcagc                                                                                                        >  2:414465/1‑140 (MQ=255)                        cTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCg                                                                                                             >  2:850876/1‑131 (MQ=255)                        cTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCg                                                                                                             <  1:850876/131‑1 (MQ=255)                         ttGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCcagca                                                                                                       <  1:481359/136‑1 (MQ=255)                         ttGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCcagca                                                                                                       >  2:481359/1‑136 (MQ=255)                           gCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGGCAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCt                                                                                                 >  1:625549/1‑140 (MQ=255)                               tCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGcc                                                                                                           >  2:542718/1‑126 (MQ=255)                               tCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGcc                                                                                                           <  1:542718/126‑1 (MQ=255)                               tCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACg                                                                                             <  1:736440/140‑1 (MQ=255)                                 aaCACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACgct                                                                                           >  1:940778/1‑140 (MQ=255)                                      acCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTg                                                                                                   <  1:817195/127‑1 (MQ=255)                                      acCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTg                                                                                                   >  2:817195/1‑127 (MQ=255)                                      acCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCttt                                                                                      >  2:872356/1‑140 (MQ=255)                                      acCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCttt                                                                                      >  2:982348/1‑140 (MQ=255)                                         aGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTtgtg                                                                                   >  1:647170/1‑140 (MQ=255)                                         aGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTtgtg                                                                                   <  2:940778/140‑1 (MQ=255)                                           ttctggtgtgtgtgtGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGaa                                                                                 <  1:59333/133‑1 (MQ=255)                                              gACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAagag                                                                              <  2:126883/140‑1 (MQ=255)                                                  gtgtgtgtGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACg                                                                          >  2:796883/1‑140 (MQ=255)                                                  gtgtgtgtGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACg                                                                          >  2:890801/1‑140 (MQ=255)                                                  gtgtgtgtGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACg                                                                          >  2:781332/1‑140 (MQ=255)                                                  gtgtgtgtGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACg                                                                          >  1:824593/1‑140 (MQ=255)                                                   tgtgtgtgGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGa                                                                         >  1:2071/1‑140 (MQ=255)                                                    gtgtgtgGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAagag                                                                              <  2:747102/134‑1 (MQ=255)                                                    gtgtgtgGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAagag                                                                              >  1:747102/1‑134 (MQ=255)                                                         tgGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATgc                                                                   >  1:941543/1‑140 (MQ=255)                                                           ggCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTAc                                                                           <  2:605068/130‑1 (MQ=255)                                                           ggCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTAc                                                                           >  1:605068/1‑130 (MQ=255)                                                               cTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATTTCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCaaa                                                             >  2:785454/1‑140 (MQ=255)                                                               cTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGcc                                                                >  2:103203/1‑137 (MQ=255)                                                               cTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGcc                                                                <  1:103203/137‑1 (MQ=255)                                                               cTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCaaa                                                             <  1:670395/140‑1 (MQ=255)                                                               cTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCaaa                                                             >  1:112561/1‑140 (MQ=255)                                                               cTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCaa                                                              <  1:108436/139‑1 (MQ=255)                                                               cTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCaa                                                              >  2:108436/1‑139 (MQ=255)                                                                 aaCTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACa                                                           >  1:231970/1‑140 (MQ=255)                                                                 aaCTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACa                                                           >  1:470203/1‑140 (MQ=255)                                                                 aaCTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACa                                                           >  2:570884/1‑140 (MQ=255)                                                                  aCTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAg                                                          >  2:25387/1‑140 (MQ=255)                                                                     gcggatgcggatTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTg                                                       <  1:781332/140‑1 (MQ=255)                                                                      cggatgcggatTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGt                                                      >  1:499244/1‑140 (MQ=255)                                                                         atgcggatTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCgg                                                   <  1:890801/140‑1 (MQ=255)                                                                          tgcggatTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGt                                                  <  1:570884/140‑1 (MQ=255)                                                                            cggatTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTg                                                >  2:309108/1‑140 (MQ=255)                                                                             ggatTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTgg                                               <  1:796883/140‑1 (MQ=255)                                                                              gatTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGc                                              >  1:18360/1‑140 (MQ=255)                                                                               atTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGca                                             <  1:785454/140‑1 (MQ=255)                                                                               atTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGca                                             <  2:499244/140‑1 (MQ=255)                                                                                    cTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGcactcac                                        <  2:959265/140‑1 (MQ=255)                                                                                    cTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGcactcac                                        <  2:53924/140‑1 (MQ=255)                                                                                     tGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGcactcact                                       <  2:941543/140‑1 (MQ=255)                                                                                     tGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGcactcact                                       <  2:2071/140‑1 (MQ=255)                                                                                      ggCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGcactcactc                                      <  1:414465/140‑1 (MQ=255)                                                                                       gcgcAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGcactcactca                                     <  2:647170/140‑1 (MQ=255)                                                                                         gcAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGcactcactcact                                   >  2:213572/1‑140 (MQ=255)                                                                                                gggCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGCACTCACTCACTTtgatga                            <  2:554492/140‑1 (MQ=255)                                                                                                 ggCAAGGACAGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGCACTCACTCACTTTgatgat                           >  1:734078/1‑140 (MQ=255)                                                                                                       gACAGGTTGAACAGGTCTTTGCCAGAACAGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGCACTCACTCACTTTGATGATCTGTTc                     >  1:609298/1‑140 (MQ=255)                                                                                                          aGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGCACTCACTCCCTTTGATGATCTGTTCCgg                  <  2:625549/140‑1 (MQ=255)                                                                                                          aGGTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGCACTCACTCACTTTGATGATCTGTTCCgg                  <  1:872356/140‑1 (MQ=255)                                                                                                            gTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGCACTCACTCACTTTGATGATCTGTTCCgg                  >  1:505967/1‑138 (MQ=255)                                                                                                            gTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGCACTCACTCACTTTGATGATCTGTTCCgg                  <  2:505967/138‑1 (MQ=255)                                                                                                            gTTGAACAGGTCTTTGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGCACTCACTCACTTTGATGATCTGTTCCGGCa                >  1:960308/1‑140 (MQ=255)                                                                                                                          tGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGCACTCACTCACTTTGATGATCTGTTCCGGCATGGTGATAAGGTTc  >  2:477524/1‑140 (MQ=255)                                                                                                                          tGCCAGAACGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGCACTCACTCACTTTGATGATCTGTTCCGGCATGGTGATAAGGTTc  >  1:87265/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                     CCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGCACTCACTCACTTTGATGATCTGTTCCGGCATGGTGATAAGGTTC  >  NC_000913/335872‑336132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑