Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,125,344	T→C	Q39R (CAG→CGG)	glcF ←	glycolate dehydrogenase, putative iron‑sulfur subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,125,344	0	T	C	100.0%	169.6 / NA	47	Q39R (CAG→CGG)	glcF	glycolate dehydrogenase, putative iron‑sulfur subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/23);  total (24/23)

GGTGGTTTCACAATTACGGCAAGTGAGGCAGCGATCGAGATGCTCCTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTC  >  NC_000913/3125208‑3125478                                                                                                                                         |                                                                                                                                       ggtggtTTCACAATTACGGCAAGTGAGGCAGCGATCGAGATGCTCCTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGAt                                                                                                                                     >  2:718479/1‑140 (MQ=255) ggtggtTTCACAATTACGGCAAGTGAGGCAGCGATCGAGATGCTCCTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGAt                                                                                                                                     >  1:377350/1‑140 (MQ=255)  gtggtTTCACAATTACGGCAAGTGAGGCAGCGATCGAGATGCTCCTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATa                                                                                                                                    >  2:40481/1‑140 (MQ=255)        tCACAATTACGGCAAGTGAGGCAGCGATCGAGATGCTCCTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTgg                                                                                                                              <  2:17046/140‑1 (MQ=255)         cacaATTACGGCAAGTGAGGCAGCGATCGAGATGCTCCTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTggg                                                                                                                             <  2:32610/140‑1 (MQ=255)                aCGGCAAGTGAGGCAGCGATCGAGATGCTCCTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTg                                                                                                                      >  1:156752/1‑140 (MQ=255)                      aGTGAGGCAGCGATCGAGATGCTCCTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGt                                                                                                                                 >  1:625491/1‑123 (MQ=255)                      aGTGAGGCAGCGATCGAGATGCTCCTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGt                                                                                                                                 <  2:625491/123‑1 (MQ=255)                      aGTGAGGCAGCGATCGAGATGCTCCTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTAc                                                                                                                >  1:242093/1‑140 (MQ=255)                      aGTGAGGCAGCGATCGAGATGCTCCTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTAc                                                                                                                >  1:480124/1‑140 (MQ=255)                             cAGCGATCGAGATGCTCCTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATcc                                                                                                         <  2:642983/140‑1 (MQ=255)                              aGCGATCGAGATGCTCCTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCg                                                                                                        <  1:440729/140‑1 (MQ=255)                              aGCGATCGAGATGCTCCTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCg                                                                                                        <  2:377350/140‑1 (MQ=255)                                 gATCGAGATGCTCCTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAg                                                                                                     >  2:147912/1‑140 (MQ=255)                                  aTCGAGATGCTCCTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGt                                                                                                    <  2:242093/140‑1 (MQ=255)                                  aTCGAGATGCTCCTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATACGCCCGCGCGGCCCGTCCAGGTCAACGCCCAGAAGCCGATATGTTGGGCTGGTTTCTGTACAAAATCCGCAGt                                                                                                    <  2:828876/140‑1 (MQ=255)                                             ccTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCa                                                                                                      <  1:516288/127‑1 (MQ=255)                                             ccTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCa                                                                                                      >  2:516288/1‑127 (MQ=255)                                             ccTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGgcg                                                                                         >  1:610871/1‑140 (MQ=255)                                                      tAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATg                                                                                >  1:671660/1‑140 (MQ=255)                                                       aaGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGc                                                                               <  1:623328/140‑1 (MQ=255)                                                           gTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGgcg                                                                                         >  2:268113/1‑126 (MQ=255)                                                           gTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGgcg                                                                                         <  1:268113/126‑1 (MQ=255)                                                                      gCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAgcg                                                                <  2:406167/140‑1 (MQ=255)                                                                            cAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAgcgcgcgcg                                                          >  1:71920/1‑140 (MQ=255)                                                                               cACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGt                                                       <  1:718479/140‑1 (MQ=255)                                                                                 ccTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTc                                                     >  1:521670/1‑140 (MQ=255)                                                                                 ccTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTc                                                     <  2:458699/140‑1 (MQ=255)                                                                                     tttAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGcc                                                 >  1:433874/1‑140 (MQ=255)                                                                                     tttAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGcc                                                 >  1:401094/1‑140 (MQ=255)                                                                                         aTCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCAt                                             <  2:521670/140‑1 (MQ=255)                                                                                                   tGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGc                                               >  1:629494/1‑128 (MQ=255)                                                                                                   tGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGc                                               <  2:629494/128‑1 (MQ=255)                                                                                                    gcgcCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGc                                               <  1:575688/127‑1 (MQ=255)                                                                                                    gcgcCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGc                                               >  2:575688/1‑127 (MQ=255)                                                                                                      gcCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAAt                                <  2:156752/140‑1 (MQ=255)                                                                                                      gcCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAAt                                <  2:671660/140‑1 (MQ=255)                                                                                                      gcCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAAt                                <  1:40481/140‑1 (MQ=255)                                                                                                      gcCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAAt                                >  1:351778/1‑140 (MQ=255)                                                                                                       cccGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAAt                                <  2:947556/139‑1 (MQ=255)                                                                                                       cccGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAAt                                >  1:947556/1‑139 (MQ=255)                                                                                                        ccGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAAt                                <  1:901348/138‑1 (MQ=255)                                                                                                        ccGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAAt                                >  2:901348/1‑138 (MQ=255)                                                                                                          gcgcgGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTggg                            <  1:147912/140‑1 (MQ=255)                                                                                                           cgcgGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTc                                       <  2:187605/128‑1 (MQ=255)                                                                                                           cgcgGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTc                                       >  1:187605/1‑128 (MQ=255)                                                                                                                              cGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCa        >  1:488989/1‑140 (MQ=255)                                                                                                                                    gAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTc  >  1:361976/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       GGTGGTTTCACAATTACGGCAAGTGAGGCAGCGATCGAGATGCTCCTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTC  >  NC_000913/3125208‑3125478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑