Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,223,947	C→T	P439S (CCG→TCG)	ebgA →	evolved beta‑D‑galactosidase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,223,947	0	C	T	100.0%	270.9 / NA	77	P439S (CCG→TCG)	ebgA	evolved beta‑D‑galactosidase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/42);  total (35/42)

TTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGT  >  NC_000913/3223810‑3224080                                                                                                                                          |                                                                                                                                      ttGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCg                                                                                                                                     >  1:183310/1‑140 (MQ=255)  tgtgATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGt                                                                                                                                    <  1:618212/140‑1 (MQ=255)  tgtgATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGt                                                                                                                                    <  2:703608/140‑1 (MQ=255)  tgtgATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGt                                                                                                                                    <  2:674361/140‑1 (MQ=255)  tgtgATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGt                                                                                                                                    <  2:670808/140‑1 (MQ=255)   gtgATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTc                                                                                                                                   <  1:46972/140‑1 (MQ=255)     gATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGa                                                                                                                                 <  2:47739/140‑1 (MQ=255)     gATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGa                                                                                                                                 <  2:588998/140‑1 (MQ=255)        ggCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGatca                                                                                                                              >  2:96571/1‑140 (MQ=255)          cGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGatcatc                                                                                                                            >  1:956082/1‑140 (MQ=255)          cGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCACCATATCCACGCGCAGAAAAACCATTCGTCGat                                                                                                                                <  1:255911/136‑1 (MQ=255)          cGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCACCATATCCACGCGCAGAAAAACCATTCGTCGat                                                                                                                                >  2:255911/1‑136 (MQ=255)                   aCGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCg                                                                                                                   <  2:365850/140‑1 (MQ=255)                     gTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCt                                                                                                                 <  1:140286/140‑1 (MQ=255)                      tCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTg                                                                                                                <  2:713904/140‑1 (MQ=255)                            cGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAAt                                                                                                          <  2:956082/140‑1 (MQ=255)                                cGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAAt                                                                                                      <  2:355823/140‑1 (MQ=255)                                cGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAAt                                                                                                      <  2:332235/140‑1 (MQ=255)                                   cTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCg                                                                                                   >  1:65573/1‑140 (MQ=255)                                     ttGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGc                                                                                                 <  2:8247/140‑1 (MQ=255)                                      tGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCt                                                                                                <  1:592493/140‑1 (MQ=255)                                      tGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCt                                                                                                <  1:96571/140‑1 (MQ=255)                                         tAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATg                                                                                             <  1:446120/140‑1 (MQ=255)                                            tGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCt                                                                                          >  2:807438/1‑140 (MQ=255)                                            tGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCt                                                                                          >  2:912778/1‑140 (MQ=255)                                             gTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAAt                                                                                                          >  2:596011/1‑123 (MQ=255)                                             gTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAAt                                                                                                          <  1:596011/123‑1 (MQ=255)                                             gTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTg                                                                                         >  2:399646/1‑140 (MQ=255)                                             gTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTg                                                                                         >  1:309912/1‑140 (MQ=255)                                              tCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGt                                                                                        <  2:622520/140‑1 (MQ=255)                                                ggCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAAt                                                                                                      <  2:761746/124‑1 (MQ=255)                                                ggCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAAt                                                                                                      >  1:761746/1‑124 (MQ=255)                                                ggCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTaa                                                                                      >  1:768563/1‑140 (MQ=255)                                                ggCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTaa                                                                                      >  2:65751/1‑140 (MQ=255)                                                     tatTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATcc                                                                                 <  2:183310/140‑1 (MQ=255)                                                     tatTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATcc                                                                                 <  1:101605/140‑1 (MQ=255)                                                       ttAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCcgc                                                                               <  2:768563/140‑1 (MQ=255)                                                       ttAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCcgc                                                                               >  1:635688/1‑140 (MQ=255)                                                       ttAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCcgc                                                                               >  2:447773/1‑140 (MQ=255)                                                        tAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCcgcg                                                                              <  1:912778/140‑1 (MQ=255)                                                        tAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCcgcg                                                                              <  2:184648/140‑1 (MQ=255)                                                        tAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCcgcg                                                                              <  1:807438/140‑1 (MQ=255)                                                        tAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCcgcg                                                                              <  1:233055/140‑1 (MQ=255)                                                        tAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCcgcg                                                                              >  2:466425/1‑140 (MQ=255)                                                                ttACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAAc                                                                                     <  1:41786/125‑1 (MQ=255)                                                                ttACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAAc                                                                                     >  2:41786/1‑125 (MQ=255)                                                                ttACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTAc                                                                      <  1:216956/140‑1 (MQ=255)                                                                        gaTCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATgc                                                                 >  1:164246/1‑137 (MQ=255)                                                                        gaTCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATgc                                                                 <  2:164246/137‑1 (MQ=255)                                                                           ccGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGaa                                                           >  2:938527/1‑140 (MQ=255)                                                                                   ggAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTgg                                                   >  2:399850/1‑140 (MQ=255)                                                                                     aaaaaGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCGATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGAt                                                 >  1:492691/1‑140 (MQ=255)                                                                                             tACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACAcgcg                                         >  2:737675/1‑140 (MQ=255)                                                                                              aCGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGa                                        <  2:34918/140‑1 (MQ=255)                                                                                               cGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGAc                                       <  1:447773/140‑1 (MQ=255)                                                                                                    aGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTg                                  >  2:275557/1‑140 (MQ=255)                                                                                                    aGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTg                                  >  1:63537/1‑140 (MQ=255)                                                                                                     gcgcATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGc                                 >  2:645093/1‑140 (MQ=255)                                                                                                      cgcATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCa                                <  1:65751/140‑1 (MQ=255)                                                                                                        cATTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCGATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCAtt                              <  2:492691/140‑1 (MQ=255)                                                                                                         aTTGTTCGCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTa                             >  2:130949/1‑140 (MQ=255)                                                                                                                gCCATATCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACgaagaa                      <  2:635688/140‑1 (MQ=255)                                                                                                                     atCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATcg                 >  2:452195/1‑140 (MQ=255)                                                                                                                     atCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATcg                 >  2:657223/1‑140 (MQ=255)                                                                                                                     atCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATcg                 <  2:309912/140‑1 (MQ=255)                                                                                                                     atCCACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATcg                 <  1:645093/140‑1 (MQ=255)                                                                                                                       ccACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATcgcg               >  1:652208/1‑140 (MQ=255)                                                                                                                       ccACGCGCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATcgcg               >  2:129371/1‑140 (MQ=255)                                                                                                                          cgcgCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATg            <  2:520607/140‑1 (MQ=255)                                                                                                                          cgcgCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATg            >  1:520607/1‑140 (MQ=255)                                                                                                                          cgcgCAGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATACGGCGAAAGCGCTGGATGATACGCGACTGGTGCATTACGAAGAAGATCGCGATg            >  2:285794/1‑140 (MQ=255)                                                                                                                               aGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGaa       >  2:853971/1‑140 (MQ=255)                                                                                                                               aGAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGaa       >  2:898397/1‑140 (MQ=255)                                                                                                                                gAAAAACCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAg      >  2:821962/1‑140 (MQ=255)                                                                                                                                    aaCCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGt  <  2:652208/140‑1 (MQ=255)                                                                                                                                    aaCCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGt  <  1:737675/140‑1 (MQ=255)                                                                                                                                    aaCCATTCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGt  <  1:130949/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      TTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGT  >  NC_000913/3223810‑3224080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑