Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,236,458	A→G	I167V (ATC→GTC)	ygjP →	putative metal‑dependent hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,236,458	0	A	G	100.0%	192.6 / NA	53	I167V (ATC→GTC)	ygjP	putative metal‑dependent hydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/25);  total (28/25)

CGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAATCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGA  >  NC_000913/3236322‑3236591                                                                                                                                         |                                                                                                                                      cGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCt                                                                                                                                    >  1:362314/1‑140 (MQ=255)  gAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTg                                                                                                                                   >  2:746484/1‑140 (MQ=255)       ggCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCt                                                                                                                                    >  2:357808/1‑134 (MQ=255)       ggCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCt                                                                                                                                    <  1:357808/134‑1 (MQ=255)       ggCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAat                                                                                                                              >  2:660195/1‑140 (MQ=255)       ggCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAat                                                                                                                              <  1:746484/140‑1 (MQ=255)              cTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCa                                                                                                                       >  2:706627/1‑140 (MQ=255)                        aaGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTAtct                                                                                                             >  2:543776/1‑140 (MQ=255)                           agCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAATCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGc                                                                                                                    >  1:299007/1‑130 (MQ=255)                           agCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAATCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGc                                                                                                                    <  2:299007/130‑1 (MQ=255)                             cATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTAtcttttct                                                                                                        <  1:660195/140‑1 (MQ=255)                                 aCAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTAt                                                                                                               >  2:356592/1‑129 (MQ=255)                                 aCAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTAt                                                                                                               <  1:356592/129‑1 (MQ=255)                                     aGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAg                                                                                                >  2:63578/1‑140 (MQ=255)                                                   tgtgttgtCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGAc                                                                                  <  1:45255/140‑1 (MQ=255)                                                   tgtgttgtCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGAc                                                                                  <  1:370980/140‑1 (MQ=255)                                                   tgtgttgtCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGAc                                                                                  <  1:706627/140‑1 (MQ=255)                                                       ttgtCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTcc                                                                              <  2:463899/140‑1 (MQ=255)                                                       ttgtCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTcc                                                                              >  2:517383/1‑140 (MQ=255)                                                        tgtCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTccc                                                                             <  2:931350/140‑1 (MQ=255)                                                        tgtCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTccc                                                                             <  2:389715/140‑1 (MQ=255)                                                             tatGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCtt                                                                        <  1:575824/140‑1 (MQ=255)                                                             tatGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCtt                                                                        <  2:362314/140‑1 (MQ=255)                                                                 gAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGt                                                                    >  1:543128/1‑140 (MQ=255)                                                                    ccccAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTaa                                                                 >  2:296682/1‑140 (MQ=255)                                                                    ccccAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTaa                                                                 >  1:315969/1‑140 (MQ=255)                                                                          tACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATaa                                                           <  1:63578/140‑1 (MQ=255)                                                                          tACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATaa                                                           <  2:315969/140‑1 (MQ=255)                                                                            ccaccaGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACg                                                         >  2:804606/1‑140 (MQ=255)                                                                            ccaccaGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACg                                                         >  1:351846/1‑140 (MQ=255)                                                                               ccaGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCtt                                                                        >  1:715360/1‑122 (MQ=255)                                                                               ccaGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCtt                                                                        <  2:715360/122‑1 (MQ=255)                                                                               ccaGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGAcc                                                      >  1:668168/1‑140 (MQ=255)                                                                                  gCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCAtg                                                   >  1:944816/1‑140 (MQ=255)                                                                                      gAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCAtgctgc                                               <  1:517383/140‑1 (MQ=255)                                                                                      gAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCAtgctgc                                               <  2:543128/140‑1 (MQ=255)                                                                                          tCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTgcgcgc                                           >  1:518829/1‑140 (MQ=255)                                                                                            gACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCAt                                         >  2:867919/1‑140 (MQ=255)                                                                                                cccGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGc                                     <  2:351846/140‑1 (MQ=255)                                                                                                        tgGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCcttc                             >  2:398071/1‑140 (MQ=255)                                                                                                         ggCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCcttct                            >  1:198993/1‑140 (MQ=255)                                                                                                          gCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCcttctt                           >  2:462652/1‑140 (MQ=255)                                                                                                               cGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCg                      <  2:944816/140‑1 (MQ=255)                                                                                                                  aGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTAt                   >  1:6309/1‑140 (MQ=255)                                                                                                                         gCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGtttt            >  2:489088/1‑140 (MQ=255)                                                                                                                              ggCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCg       <  2:380186/140‑1 (MQ=255)                                                                                                                              ggCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCg       >  1:380186/1‑140 (MQ=255)                                                                                                                                 aGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCg    <  1:489088/140‑1 (MQ=255)                                                                                                                                  gAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCgg   <  2:198993/140‑1 (MQ=255)                                                                                                                                  gAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCgg   <  1:398071/140‑1 (MQ=255)                                                                                                                                   aaCAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCg    >  1:885420/1‑138 (MQ=255)                                                                                                                                   aaCAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCg    <  2:885420/138‑1 (MQ=255)                                                                                                                                   aaCAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGa  >  1:466040/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      CGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAATCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGA  >  NC_000913/3236322‑3236591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑