Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,243,802	G→A	P314S (CCG→TCG)	uxaC ←	D‑glucoronate/D‑galacturonate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,243,802	0	G	A	100.0%	176.9 / NA	52	P314S (CCG→TCG)	uxaC	D‑glucoronate/D‑galacturonate isomerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/23);  total (29/23)

AGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACT  >  NC_000913/3243666‑3243929                                                                                                                                         |                                                                                                                                aGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGAccc                                                                                                                              >  2:112102/1‑140 (MQ=255)  gCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGAcccc                                                                                                                             >  2:454815/1‑140 (MQ=255)   cACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCca                                                                                                                            <  1:214212/140‑1 (MQ=255)             tCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGaa                                                                                                                  >  1:976727/1‑140 (MQ=255)                   gggTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACg                                                                                                            <  1:386515/140‑1 (MQ=255)                   gggTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACg                                                                                                            <  1:543978/140‑1 (MQ=255)                        cAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGgt                                                                                                       <  2:448979/140‑1 (MQ=255)                               tACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCa                                                                                                          <  1:439850/130‑1 (MQ=255)                               tACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCa                                                                                                          >  2:439850/1‑130 (MQ=255)                               tACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGttatt                                                                                                >  1:137664/1‑140 (MQ=255)                               tACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGttatt                                                                                                >  2:555901/1‑140 (MQ=255)                                    aaTGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCa                                                                                           >  2:859164/1‑140 (MQ=255)                                    aaTGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCa                                                                                           >  2:221901/1‑140 (MQ=255)                                       ggTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAgcg                                                                                        <  1:859164/140‑1 (MQ=255)                                         ttttCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAgcgcg                                                                                      >  1:438780/1‑140 (MQ=255)                                         ttttCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAgcgcg                                                                                      >  1:113458/1‑140 (MQ=255)                                          tttCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAgcgcgc                                                                                     <  2:976727/140‑1 (MQ=255)                                               gcagcaGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAAtg                                                                                <  1:454815/140‑1 (MQ=255)                                                   cagGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAAtg                                                                                <  2:89311/136‑1 (MQ=255)                                                   cagGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAAtg                                                                                >  1:89311/1‑136 (MQ=255)                                                    agGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTa                                                                           <  1:112102/140‑1 (MQ=255)                                                    agGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTa                                                                           <  1:555901/140‑1 (MQ=255)                                                         ttcttcATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTActgct                                                                      >  2:17703/1‑140 (MQ=255)                                                             tcATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGTAc                                                                  >  1:701208/1‑140 (MQ=255)                                                                ttCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGTACCCa                                                               >  2:904525/1‑140 (MQ=255)                                                                ttCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGTACCCa                                                               >  2:514062/1‑140 (MQ=255)                                                                ttCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGTACCCa                                                               >  1:303147/1‑140 (MQ=255)                                                                 tCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATTGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGTACCCAg                                                              >  1:242382/1‑140 (MQ=255)                                                                 tCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGTACCCAg                                                              >  2:397559/1‑140 (MQ=255)                                                                 tCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGTACCCAg                                                              >  2:346051/1‑140 (MQ=255)                                                                     cttgctCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTActg                                                                        <  1:751890/126‑1 (MQ=255)                                                                     cttgctCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTActg                                                                        >  2:751890/1‑126 (MQ=255)                                                                     cttgctCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGTACCCAGCcgc                                                          >  2:350884/1‑140 (MQ=255)                                                                     cttgctCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGTACCCAGCcgc                                                          >  1:399842/1‑140 (MQ=255)                                                                      ttgctCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGTACCCAGCcgcg                                                         <  1:17703/140‑1 (MQ=255)                                                                               aaCTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGTACCCAGCCGCGACGTGCata                                                <  1:350884/140‑1 (MQ=255)                                                                                 cTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGTACCCAGCCGCGACGTGCATAtt                                              <  1:904525/140‑1 (MQ=255)                                                                                         gCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGTACCCAGCCGCGACGTGCATATTCGGCACCa                                      <  1:346051/140‑1 (MQ=255)                                                                                          ctctTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGTACCCAGCCGCGACGTGCATAtt                                              >  1:434461/1‑131 (MQ=255)                                                                                          ctctTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGTACCCAGCCGCGACGTGCATAtt                                              <  2:434461/131‑1 (MQ=255)                                                                                          ctctTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGTACCCAGCCGCGACGTGCATATTCGGCACCaa                                     >  1:36701/1‑140 (MQ=255)                                                                                          ctctTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGTACCCAGCCGCGACGTGCATATTCGGCACCaa                                     >  2:23142/1‑140 (MQ=255)                                                                                           tctTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGTACCCAGCCGCGACGTGCATATTCGGCACCAAg                                    >  2:851591/1‑140 (MQ=255)                                                                                            ctTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGTACCCAGCCGCGACGTGCATATTCGGCACCAAgg                                   <  2:36701/140‑1 (MQ=255)                                                                                            ctTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGTACCCAGCCGCGACGTGCATATTCGGCACCAAgg                                   <  1:397559/140‑1 (MQ=255)                                                                                             ttCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGa                                  <  1:514062/140‑1 (MQ=255)                                                                                               ccGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAac                                >  1:143757/1‑140 (MQ=255)                                                                                                         aCGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCg                      <  2:137664/140‑1 (MQ=255)                                                                                                         aCGGTCGTTGATGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCg                      <  2:701208/140‑1 (MQ=255)                                                                                                                   aTGGAGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGaa            <  2:242382/140‑1 (MQ=255)                                                                                                                       aGTCAAAGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTgc        >  1:770300/1‑140 (MQ=255)                                                                                                                             aGCCTACATCCGACCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACt  >  1:311838/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                AGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACT  >  NC_000913/3243666‑3243929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑