Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,246,318	A→G	L365L (TTA→TTG)	exuT →	hexuronate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,246,318	0	A	G	100.0%	220.4 / NA	61	L365L (TTA→TTG)	exuT	hexuronate transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/33);  total (28/33)

GCCTGTTCACCAACCCGTATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCG  >  NC_000913/3246182‑3246454                                                                                                                                         |                                                                                                                                         gCCTGTTCACCAACCCGTATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGAcc                                                                                                                                       >  1:828539/1‑140 (MQ=255) gCCTGTTCACCAACCCGTATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGAcc                                                                                                                                       >  2:952720/1‑140 (MQ=255) gCCTGTTCACCAACCCGTATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGAcc                                                                                                                                       >  1:634481/1‑140 (MQ=255)                   aTGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCAtggctg                                                                                                                     >  1:714892/1‑140 (MQ=255)                       cGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCaa                                                                                                                 >  2:286024/1‑140 (MQ=255)                         cAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCa                                                                                                                  <  1:911526/137‑1 (MQ=255)                         cAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGt                                                                                                               >  2:107305/1‑140 (MQ=255)                         cAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGt                                                                                                               >  1:855586/1‑140 (MQ=255)                         cAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGACGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCa                                                                                                                  >  2:911526/1‑137 (MQ=255)                               tgctgcTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTg                                                                                                         >  1:482625/1‑140 (MQ=255)                                 ctgctgTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGtt                                                                                                       <  2:437184/140‑1 (MQ=255)                                      gtgtATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTgcgc                                                                                                  >  2:487729/1‑140 (MQ=255)                                       tgtATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCt                                                                                                 >  2:601758/1‑140 (MQ=255)                                        gtATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTg                                                                                                <  1:376961/140‑1 (MQ=255)                                         tATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTgg                                                                                               >  1:798760/1‑140 (MQ=255)                                         tATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTgg                                                                                               <  1:107305/140‑1 (MQ=255)                                              gtggtTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTc                                                                                          <  1:952720/140‑1 (MQ=255)                                                 gtTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGt                                                                                       >  1:30962/1‑140 (MQ=255)                                                 gtTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGt                                                                                       >  2:916216/1‑140 (MQ=255)                                                    ttGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCa                                                                                    <  2:634481/140‑1 (MQ=255)                                                    ttGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCa                                                                                    <  1:934200/140‑1 (MQ=255)                                                     tGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCa                                                                                    >  1:605154/1‑139 (MQ=255)                                                     tGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCa                                                                                    <  2:605154/139‑1 (MQ=255)                                                                     tCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCgg                                                                   >  2:183003/1‑140 (MQ=255)                                                                             gcTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGcc                                                           >  2:640279/1‑140 (MQ=255)                                                                               tGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCg                                                         >  2:155601/1‑140 (MQ=255)                                                                               tGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCg                                                         >  2:985947/1‑140 (MQ=255)                                                                               tGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCg                                                         <  1:192489/140‑1 (MQ=255)                                                                               tGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCg                                                         <  1:707834/140‑1 (MQ=255)                                                                                      gCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCg                                                  <  1:916216/140‑1 (MQ=255)                                                                                       ctctctTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGc                                                 <  1:485275/140‑1 (MQ=255)                                                                                           ctTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCg                                                         <  2:654595/128‑1 (MQ=255)                                                                                           ctTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCg                                                         >  1:654595/1‑128 (MQ=255)                                                                                           ctTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGtt                                             <  2:483202/140‑1 (MQ=255)                                                                                           ctTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGtt                                             <  2:176885/140‑1 (MQ=255)                                                                                             tCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCt                                           >  2:452487/1‑140 (MQ=255)                                                                                               cGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTgg                                         <  2:258428/140‑1 (MQ=255)                                                                                               cGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTgg                                         <  1:286024/140‑1 (MQ=255)                                                                                               cGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTgg                                         <  2:30962/140‑1 (MQ=255)                                                                                               cGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTgg                                         <  1:985947/140‑1 (MQ=255)                                                                                               cGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTgg                                         <  1:487729/140‑1 (MQ=255)                                                                                                gATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGc                                        <  2:482625/140‑1 (MQ=255)                                                                                                 aTGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCa                                       >  1:167133/1‑140 (MQ=255)                                                                                                    tgtTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAgtg                                    >  1:769286/1‑140 (MQ=255)                                                                                                    tgtTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAgtg                                    >  1:476186/1‑140 (MQ=255)                                                                                                              tAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTc                                 <  1:831979/133‑1 (MQ=255)                                                                                                              tAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTc                                 >  2:831979/1‑133 (MQ=255)                                                                                                              tAACGAAGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGt                          >  2:824414/1‑140 (MQ=255)                                                                                                                    aGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTg                    <  2:476186/140‑1 (MQ=255)                                                                                                                    aGTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTg                    <  1:155601/140‑1 (MQ=255)                                                                                                                     gTGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTgc                   <  1:824414/140‑1 (MQ=255)                                                                                                                      tGGCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTgcg                  <  1:601758/140‑1 (MQ=255)                                                                                                                        gCAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCt                >  1:852226/1‑140 (MQ=255)                                                                                                                         cAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTg               <  1:640279/140‑1 (MQ=255)                                                                                                                         cAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTg               <  2:892610/140‑1 (MQ=255)                                                                                                                         cAACGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCACGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTg               <  1:829491/140‑1 (MQ=255)                                                                                                                           aCGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGt             <  2:714892/140‑1 (MQ=255)                                                                                                                           aCGGCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGt             <  2:167133/140‑1 (MQ=255)                                                                                                                             ggCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGtt                         >  1:894856/1‑126 (MQ=255)                                                                                                                             ggCAAACGGTTTGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGtt                         <  2:894856/126‑1 (MQ=255)                                                                                                                                      tttGACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCg  <  2:769286/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         GCCTGTTCACCAACCCGTATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCG  >  NC_000913/3246182‑3246454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑