Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,256,262	T→C	S130S (TCA→TCG)	cyuA ←	putative L‑cysteine desulfidase CyuA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,256,262	0	T	C	100.0%	149.2 / NA	43	S130S (TCA→TCG)	cyuA	putative L‑cysteine desulfidase CyuA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/23);  total (20/23)

ACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCAC  >  NC_000913/3256126‑3256395                                                                                                                                         |                                                                                                                                      aCTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCgag                                                                                                                                    <  1:799044/140‑1 (MQ=255)          cGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGAtt                                                                                                                           >  1:160206/1‑140 (MQ=255)              ctctGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCAt                                                                                                                       <  1:82816/140‑1 (MQ=255)                  gCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCa                                                                                                                   <  1:177053/140‑1 (MQ=255)                  gCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCa                                                                                                                   <  2:753893/140‑1 (MQ=255)                  gCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCa                                                                                                                   <  1:938694/140‑1 (MQ=255)                  gCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCa                                                                                                                   <  1:77023/140‑1 (MQ=255)                          cGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCt                                                                                                           <  2:751862/140‑1 (MQ=255)                          cGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCt                                                                                                           <  1:767547/140‑1 (MQ=255)                                     tAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACg                                                                                                >  2:65978/1‑140 (MQ=255)                                                   tCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCccgc                                                                                  >  1:434904/1‑140 (MQ=255)                                                      tGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACATACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCcgccgc                                                                               >  2:980262/1‑140 (MQ=255)                                                      tGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACg                                                                                                <  2:852873/123‑1 (MQ=255)                                                      tGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACg                                                                                                >  1:852873/1‑123 (MQ=255)                                                          tctcGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCcagc                                                                           >  2:550411/1‑140 (MQ=255)                                                          tctcGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCcagc                                                                           <  1:65978/140‑1 (MQ=255)                                                           ctcGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCcagca                                                                          <  1:916810/140‑1 (MQ=255)                                                                   gCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTg                                                                  <  2:434904/140‑1 (MQ=255)                                                                    cacaATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTgg                                                                 >  2:176285/1‑140 (MQ=255)                                                                          gTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCcag                                                                            >  2:721346/1‑123 (MQ=255)                                                                          gTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCcag                                                                            <  1:721346/123‑1 (MQ=255)                                                                                     cgccgACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTgc                                                <  2:160206/140‑1 (MQ=255)                                                                                                      cacGCCCACTTCTCCCCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAg                               <  2:19329/140‑1 (MQ=255)                                                                                                         gCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCAc                            >  1:803524/1‑140 (MQ=255)                                                                                                          cccACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACt                           >  1:379779/1‑140 (MQ=255)                                                                                                          cccACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACt                           >  2:501815/1‑140 (MQ=255)                                                                                                           ccACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACtt                          <  2:379779/140‑1 (MQ=255)                                                                                                              cTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCa                       >  2:637266/1‑140 (MQ=255)                                                                                                                 ctcACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGGCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAg                               <  2:738633/129‑1 (MQ=255)                                                                                                                 ctcACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAg                               >  1:483563/1‑129 (MQ=255)                                                                                                                 ctcACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAg                               <  2:483563/129‑1 (MQ=255)                                                                                                                 ctcACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAg                               >  1:738633/1‑129 (MQ=255)                                                                                                                 ctcACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGcc                    <  2:803524/140‑1 (MQ=255)                                                                                                                     ccGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGc                <  1:637266/140‑1 (MQ=255)                                                                                                                         tCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTg            >  2:74078/1‑140 (MQ=255)                                                                                                                         tCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTg            <  1:149952/140‑1 (MQ=255)                                                                                                                         tCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTg            >  2:426776/1‑140 (MQ=255)                                                                                                                         tCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTg            >  2:149952/1‑140 (MQ=255)                                                                                                                         tCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTg            >  1:976713/1‑140 (MQ=255)                                                                                                                                  tGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTgg           >  2:745426/1‑132 (MQ=255)                                                                                                                                  tGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTgg           <  1:745426/132‑1 (MQ=255)                                                                                                                                  tGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCAt        >  2:287717/1‑135 (MQ=255)                                                                                                                                  tGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCAt        <  1:287717/135‑1 (MQ=255)                                                                                                                                   ggCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCAc  <  1:426776/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      ACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCAC  >  NC_000913/3256126‑3256395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑