Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,294,906	A→G	T503A (ACT→GCT)	lpoA →	outer membrane lipoprotein ‑ activator of MrcA activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,294,906	0	A	G	100.0%	92.7 / NA	27	T503A (ACT→GCT)	lpoA	outer membrane lipoprotein ‑ activator of MrcA activity
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/23);  total (4/23)

GTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTTCTGGT‑ATTGCTTTAACGGGTAGCCCGATTACTCTCAGAGCGACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGTTCACCAATAATGGCGGTCGTGTCGATGCGGTGTACATTGTGGCAACGCCGGGTG  >  NC_000913/3294794‑3295035                                                                                                                  |                                                                                                                                  gTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTTCTGGT‑ATTGCTTTAACGGGTAGCCCGATTGCTCTCAGAGCGACAACCGACTCCGGCa                                                                                                        <  1:402819/140‑1 (MQ=255)         aaCTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTTCTGGT‑ATTGCTTTAACGGGTAGCCCGATTGCTCTCAGAGCGACAACCGACTCCGGCATGACGAcc                                                                                                <  1:951344/140‑1 (MQ=255)           cTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTTCTGGT‑ATTGCTTTAACGGGTAGCCCGATTGCTCTCAGAGCGACAACCGACTCCGGCATGACGACcaa                                                                                              <  2:693153/140‑1 (MQ=255)                   cggcACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTTTTTGTGTTTTTTTTAATGGGT‑GGCCGCGTGCTCTCCGAGCGGCAACCCACCCCCCCCTGACGACcaacaaaacaa                                                                                      >  2:357268/1‑135 (MQ=255)                       aCCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTTCTGGT‑ATTGCTTTAACGGGTAGCCCGATTGCTCTCAGAGCGACAACCGACTCCGGCATGACGACCAACAATCCAACGCt                                                                                  <  1:276129/140‑1 (MQ=255)                        ccGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTTCTGGT‑ATTGCTTTAACGGGTAGCCCGATTGCTCTCAGAGCGACAACCGACTCCGGCATGACGACCAACAATCCAACGCTg                                                                                 <  1:513610/140‑1 (MQ=255)                           ttCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTTCTGGT‑ATTGCTTTAACGGGTAGCCCGATTGCTCTCAGAGCGACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCaa                                                                              <  2:830788/140‑1 (MQ=255)                                   caaAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTTCTGGT‑ATTGCTTTAACGGGTAGCCCGATTGCTCTCAGAGCGACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGcc                                                                      <  1:590513/140‑1 (MQ=255)                                     aaaaTTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTTCTGGT‑ATTGCTTTAACGGGTAGCCCGATTGCTCTCAGAGCGACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCaa                                                                    <  2:521592/140‑1 (MQ=255)                                        aTTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTTCTGGT‑ATTGCTTTAACGGGTAGCCCGATTGCTCTCAGAGCGACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCg                                                                 <  1:691877/140‑1 (MQ=255)                                        aTTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTTCTGGT‑ATTGCTTTAACGGGTAGCCCGATTGCTCTCAGAGCGACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCg                                                                 <  1:862975/140‑1 (MQ=255)                                             gTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTTCTGGT‑ATTGCTTTAACGGGTAGCCGGATTGCTCTCAGAGCGACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGAc                                                            <  1:950275/140‑1 (MQ=255)                                             gTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTTCTGGT‑ATTGCTTTAACGGGTAGCCCGATTGCTCTCAGAGCGACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGAc                                                            <  1:357268/140‑1 (MQ=255)                                             gTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTTCTGGT‑ATTGCTTTAACGGGTAGCCCGATTGCTCTCAGAGCGACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGAc                                                            <  1:377277/140‑1 (MQ=255)                                             gTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTTCTGGT‑ATTGCTTTAACGGGTAGCCCGATTGCTCTCAGAGCGACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGAc                                                            <  1:372661/140‑1 (MQ=255)                                              ttCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTTCTGGT‑ATTGCTTTAACGGGTAGCCCGATTGCTCTCAGAGCGACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGTAAACCACGCCAACCGATGAcc                                                           <  1:81332/140‑1 (MQ=255)                                              ttCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTTCTGGT‑ATTGCTTTAACGGGTAGCCCGATTGCTCTCAGAGCGACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGAcc                                                           <  1:949160/140‑1 (MQ=255)                                              ttCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTTCTGGT‑ATTGCTTTAACGGGTAGCCCGATTGCTCTCAGAGCGACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGAcc                                                           <  1:982818/140‑1 (MQ=255)                                                 caccaGCGAATTACGCGCGGGTGTTAACGGCGGTTCTGGT‑ATTGCTTTAACGGGTAGCCCGATTGCTCTCAGAGCGACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGt                                                        <  1:767016/140‑1 (MQ=255)                                                                         tAACGGCGGTTCTGGT‑ATTGCTTTAACGGGTAGCCCGATTGCTCTCAGAGCGACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGTTCACCAATAATGGCGGTCGTGTCg                                <  1:769639/140‑1 (MQ=255)                                                                         tAACGGCGGTTCTGGT‑ATTGCTTTAACGGGTAGCCCGATTGCTCTCAGAGCGACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGTTCACCAATAATGGCGGTCGTGTCg                                <  1:872183/140‑1 (MQ=255)                                                                           aCGGCGGTTCCGGT‑ATTGCTTTAACGGGTAGCCCGATTGCTCTCAGAGCGACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGTTCACCAATAATGGCGGTCGTGTCGAt                              <  1:675308/140‑1 (MQ=255)                                                                              gcggTTCTGGT‑ATTGCTTTAACGGGTAGCCCGATTGCTCTCAGAGCGACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGTTCACCAATAATGGCGGTCGTGTCGATGCg                           <  1:510676/140‑1 (MQ=255)                                                                                             gCTTTAACGGGTAGCCCGATTGCTCTCAGAGCGACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGTTCACCAATAATGGCGGTCGTGTCGATGCGGTGTACATTGTGGc             >  1:783071/1‑140 (MQ=255)                                                                                              cTTTAACGGGTAGCCCGATTGCTCTCAGAGCGACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGTTCACCAATAATGGCGGTCGTGTCGATGCGGTGTACATTGTGGCa            >  2:546777/1‑140 (MQ=255)                                                                                                   aCGGGTAGCCCGATTGCTCTCAGAGCGACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGTTCACCAATAATGGCGGTCGTGTCGATGCGGTGTACATTGTGGCAACGcc       <  1:546777/140‑1 (MQ=255)                                                                                                        tAGCCCGATTGCTCTCAGAGCGACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGTTCACCAATAATGGCGGTCGTGTCGATGCGGTGTACATTGTGGCAACGCCGGGTg  >  1:471239/1‑140 (MQ=255)                                                                                                        tAGCCCGATTGCTCTCAGAGCGACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGTTCACCAATAATGGCGGTCGTGTCGATGCGGGGTACATTGTGGCAACGCCggggg  >  2:797957/1‑138 (MQ=255)                                                                                                                  |                                                                                                                                  GTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTTCTGGT‑ATTGCTTTAACGGGTAGCCCGATTACTCTCAGAGCGACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGTTCACCAATAATGGCGGTCGTGTCGATGCGGTGTACATTGTGGCAACGCCGGGTG  >  NC_000913/3294794‑3295035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑