Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	340,464	G→A	R100R (CGG→CGA)	yahI →	carbamate kinase‑like protein YahI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	340,464	0	G	A	100.0%	137.6 / NA	40	R100R (CGG→CGA)	yahI	carbamate kinase‑like protein YahI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/21);  total (19/21)

CGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCGAACTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCGGCTGGCGCGTCACGGCGAGAAGAAAGCCGTCACCGTGGTGACTCAGGTGGAAGTGGATAAAAACGATCCAGGTTTTGCCCATCCCACCAAGCCCATCGGCGCATTCTTTAGTGACAGCCAGCGTG  >  NC_000913/340328‑340588                                                                                                                                         |                                                                                                                             cGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCGAACTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCGACTg                                                                                                                           >  2:777211/1‑140 (MQ=255)             tGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCGAACTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCGACTGGCGCGTCACGGc                                                                                                               <  1:768308/140‑1 (MQ=255)                         gTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCGAACTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCGACTGGCGCGTCACGGCGAGAAGAAAGcc                                                                                                   >  1:818319/1‑140 (MQ=255)                         gTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCGAACTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCGACTGGCGCGTCACGGCGAGAAGAAAGcc                                                                                                   >  1:442228/1‑140 (MQ=255)                                    gCCCACAAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCGAACTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCGACTGGCGCGTCACGGCGAGAAGAAAGCCGTCACCgtggt                                                                                        >  2:497408/1‑140 (MQ=255)                                     cccACAAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCGAACTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCGACTGGCGCGTCACGGCGAGAAGAAAGCCGTCACCgtggtg                                                                                       >  1:751751/1‑140 (MQ=255)                                       cacaAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCGAACTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCGACTGGCGCGTCACGGCGAGAAGAAAGCCGTCACCGTGGTGAc                                                                                     <  1:777211/140‑1 (MQ=255)                                       cacaAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCGAACTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCGACTGGCGCGTCACGGCGAGAAGAAAGCCGTCACCGTGGTGAc                                                                                     <  1:752449/140‑1 (MQ=255)                                               cgAAGGGCTGCCCTTAACGCCGCTGGCGAACTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCGACTGGCGCGTCACGGCGAGAAGAAAGCCGTCACCGTGGTGACTCAGGTgg                                                                             >  2:642827/1‑140 (MQ=255)                                               cgAAGGGCTGCCCTTAACGCCACTGGCGAACTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCGACTGGCGCGTCACGGCGAGAAGAAAGCCGTCACCGTGGTGACTCAGGTgg                                                                             >  2:825235/1‑140 (MQ=255)                                                 aaGGGCTGCCCTTAACGCCGCTGGCGAACTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCGACTGGCGCGTCACGGCGAGAAGAAAGCCGTCACCGTGGTGACTCAGGTGGaa                                                                           <  1:159428/140‑1 (MQ=255)                                                       tGCCCTTAACGCCGCTGGCGAACTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCGACTGGCGCGTCACGGCGAGAAGAAAGCCGTCACCGTGGTGACTCAGGTGGAAGTGGAt                                                                     <  2:751751/140‑1 (MQ=255)                                                         cccTTAACGCCGCTGGCGAACTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCGACTGGCGCGTCACGGCGAGAAGAAAGCCGTCACCGTGGTGACTCAGGTGGAAg                                                                          >  1:606564/1‑133 (MQ=255)                                                         cccTTAACGCCGCTGGCGAACTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCGACTGGCGCGTCACGGCGAGAAGAAAGCCGTCACCGTGGTGACTCAGGTGGAAg                                                                          <  2:606564/133‑1 (MQ=255)                                                            ttAACGCCGCTGGCGAACTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCGACTGGCGCGTCACGGCGAGAAGAAAGCCGTCACCGTGGTGACTCAGGTGGAAGTGGATaaaaa                                                                <  2:699730/140‑1 (MQ=255)                                                              aaCGCCGCTGGCGAACTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCGACTGGCGCGTCACGGCGAGAAGAAAGCCGTCACCGTGGTGACTCAGGTGGaa                                                                           >  1:464615/1‑127 (MQ=255)                                                              aaCGCCGCTGGCGAACTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCGACTGGCGCGTCACGGCGAGAAGAAAGCCGTCACCGTGGTGACTCAGGTGGaa                                                                           <  2:464615/127‑1 (MQ=255)                                                                 gccgcTGGCGAACTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCGACTGGCGCGTCACGGCGAGAAGAAAGCCGTCACCGTGGTGACTCAGGTGGAAGTGGATAAAAACGATc                                                           <  2:437643/140‑1 (MQ=255)                                                                           aaCTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCGACTGGCGCGTCACGGCGAGAAGAAAGCCGTCACCGTGGTGACTCAGGTGGAAGTGGATAAAAACGATCCAGGTTTTGc                                                 <  2:818319/140‑1 (MQ=255)                                                                               gtgtgGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCGACTGGCGCGTCACGGCGAGAAGAAAGCCGTCACCGTGGTGACTCAGGTGGAAGTGGATAAAAACGATCCAGGTTTTGCCCAt                                             <  1:876276/140‑1 (MQ=255)                                                                               gtgtgGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCGACTGGCGCGTCACGGCGAGAAGAAAGCCGTCACCGTGGTGACTCAGGTGGAAGTGGATAAAAACGATCCAGGTTTTGCCCAt                                             <  1:497408/140‑1 (MQ=255)                                                                                tgtgGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCGACTGGCGCGTCACGGCGAGAAGAAAGCCGTCACCGTGGTGACTCAGGTGGAAGTGGATAAAAACGATcc                                                          <  2:275316/126‑1 (MQ=255)                                                                                tgtgGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCGACTGGCGCGTCACGGCGAGAAGAAAGCCGTCACCGTGGTGACTCAGGTGGAAGTGGATAAAAACGATcc                                                          >  1:275316/1‑126 (MQ=255)                                                                                        aTACGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCGACTGGCGCGTCACGGCGAGAAGAAAGCCGTCACCGTGGTGACTCAGGTGGAAGTGGATAAAAACGATCCAGGTTTTGCCCATCCCACCAAg                                    >  1:28544/1‑140 (MQ=255)                                                                                          aCGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCGACTGGCGCGTCACGGCGAGAAGAAAGCCGTCACCGTGGTGACTCAGGTGGAAGTGGATAAAAACGATCCAGGTTTTGCCCATCCCACCAAGcc                                  >  1:132009/1‑140 (MQ=255)                                                                                            gCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCGACTGGCGCGTCACGGCGAGAAGAAAGCCGTCACCGTGGTGACTCAGGTGGAAGTGGATAAAAACGATCCAGGTTTTGCCCATCCCACCAAGCCCa                                <  2:28544/140‑1 (MQ=255)                                                                                                    gcATCGGCTATCTGATCCAACAGGCGCTGAATAACCGACTGGCGCGTCACGGCGAGAAGAAAGCCGTCACCGTGGTGACTCAGGTGGAAGTGGATAAAAACGATCCAGGTTTTGCCCATCCCACCAAGCCCATCGGCGCa                        <  2:442228/140‑1 (MQ=255)                                                                                                     cATCGGCTATCTGATCCAACAGGCGCTGAATAACCGACTGGCGCGTCACGGCGAGAAGAAAGCCGTCACCGTGGTGACTCAGGTGGAAGTGGATAAAAACGATCCAGGTTTTGCCCATCCCACCAAGCCCATCGGCGCa                        <  2:247800/139‑1 (MQ=255)                                                                                                     cATCGGCTATCTGATCCAACAGGCGCTGAATAACCGACTGGCGCGTCACGGCGAGAAGAAAGCCGTCACCGTGGTGACTCAGGTGGAAGTGGATAAAAACGATCCAGGTTTTGCCCATCCCACCAAGCCCATCGGCGCa                        >  1:247800/1‑139 (MQ=255)                                                                                                          gCTATCTGATCCAACAGGCGCTGAATAACCGACTGGCGCGTCACGGCGAGAAGAAAGCCGTCACCGTGGTGACTCAGGTGGAAGTGGATAAAAACGATCCAGGTTTTGCCCATCCCACCAAGCCCAt                               <  1:267683/127‑1 (MQ=255)                                                                                                          gCTATCTGATCCAACAGGCGCTGAATAACCGACTGGCGCGTCACGGCGAGAAGAAAGCCGTCACCGTGGTGACTCAGGTGGAAGTGGATAAAAACGATCCAGGTTTTGCCCATCCCACCAAGCCCAt                               >  2:267683/1‑127 (MQ=255)                                                                                                                   tCCAACAGGCGCTGAATAACCGACTGGCGCGTCACGGCGAGAAGAAAGCCGTCACCGTGGTGACTCAGGTGGAAGTGGATAAAAACGATCCAGGTTTTGCCCATCCCACCAAGCCCATCGGCGCATTCTTTAGTGAcagc         >  1:303393/1‑140 (MQ=255)                                                                                                                   tCCAACAGGCGCTGAATAACCGACTGGCGCGTCACGGCGAGAAGAAAGCCGTCACCGTGGTGACTCAGGTGGAAGTGGATAAAAACGATCCAGGTTTTGCCCATCCCACCAAGCCCATCGGCGCATTCTTTAGTGAcagc         >  1:717608/1‑140 (MQ=255)                                                                                                                   tCCAACAGGCGCTGAATAACCGACTGGCGCGTCACGGCGAGAAGAAAGCCGTCACCGTGGTGACTCAGGTGGAAGTGGATAAAAACGATCCAGGTTTTGCCCATCCCACCAAGCCCATCGGCGCATTCTTTAGTGAcagc         >  1:196170/1‑140 (MQ=255)                                                                                                                   tCCAACAGGCGCTGAATAACCGACTGGCGCGTCACGGCGAGAAGAAAGCCGTCACCGTGGTGACTCAGGTGGAAGTGGATAAAAACGATCCAGGTTTTGCCCATCCCACCAAGCCCATCGGCGCATTCTTTAGTGAcagc         <  2:196170/140‑1 (MQ=255)                                                                                                                          ggCGCTGAATAACCGACTGGCGCGTCACGGCGAGAAGAAAGCCGTCACCGTGGTGACTCAGGTGGAAGTGGATAAAAACGATCCAGGTTTTGCCCATCCCACCAAGCCCATCGGCGCATTCTTTAGTGACAGCCAGCGTg  <  1:908718/140‑1 (MQ=255)                                                                                                                            cgcTGAATAACCGACTGGCGCGTCACGGCGAGAAGAAAGCCGTCACCGTGGTGACTCAGGTGGAAGTGGATAAAAACGATCCAGGTTTTGCCCATCCCACCAAGCCCATCGGCGCATTCTTTAGt               <  2:646975/125‑1 (MQ=255)                                                                                                                            cgcTGAATAACCGACTGGCGCGTCACGGCGAGAAGAAAGCCGTCACCGTGGTGACTCAGGTGGAAGTGGATAAAAACGATCCAGGTTTTGCCCATCCCACCAAGCCCATCGGCGCATTCTTTAGt               >  1:646975/1‑125 (MQ=255)                                                                                                                                 aaTAACCGACTGGCGCGTCACGGCGAGAAGAAAGCCGTCACCGTGGTGACTCAGGTGGAAGTGGATAAAAACGATCCAGGTTTTGCCCATCCCACCAAGCCCATCGGCGCATTCTTTAGTGACAGCCAGCg    >  2:389175/1‑131 (MQ=255)                                                                                                                                 aaTAACCGACTGGCGCGTCACGGCGAGAAGAAAGCCGTCACCGTGGTGACTCAGGTGGAAGTGGATAAAAACGATCCAGGTTTTGCCCATCCCACCAAGCCCATCGGCGCATTCTTTAGTGACAGCCAGCg    <  1:389175/131‑1 (MQ=255)                                                                                                                                         |                                                                                                                             CGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCGAACTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCGGCTGGCGCGTCACGGCGAGAAGAAAGCCGTCACCGTGGTGACTCAGGTGGAAGTGGATAAAAACGATCCAGGTTTTGCCCATCCCACCAAGCCCATCGGCGCATTCTTTAGTGACAGCCAGCGTG  >  NC_000913/340328‑340588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑