Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,419,006	T→C	intergenic (+395/‑36)	yhdV → / → yhdW	lipoprotein YhdV/putative ABC transporter periplasmic binding protein YhdW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,419,006	0	T	C	100.0%	142.3 / NA	40	intergenic (+395/‑36)	yhdV/yhdW	lipoprotein YhdV/putative ABC transporter periplasmic binding protein YhdW
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/22);  total (18/22)

CGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGCCGTTCAGAAAAA  >  NC_000913/3418883‑3419141                                                                                                                            |                                                                                                                                        cgcTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGCCCTATAACGATAAttt                                                                                                                         >  1:216280/1‑140 (MQ=255)    tGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGCCCTATAACGATAATTTTCg                                                                                                                      <  1:308442/140‑1 (MQ=255)    tGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGGGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGCCCTATAACGATAATTTTCg                                                                                                                      >  2:106858/1‑140 (MQ=255)      tttttATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGCCCTATAACGATAATTTTCGcc                                                                                                                    <  2:543750/140‑1 (MQ=255)       ttttATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGCCCTATAACGATAATTTTCGCCt                                                                                                                   <  1:733318/140‑1 (MQ=255)                  ccTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGCCCTATAACGATAATTTTCGCCTCACAGGAAGCa                                                                                                        <  1:486784/140‑1 (MQ=255)                    tGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGCCCTATAACGATAATTTTCGCCTCACAGGAAGCAtt                                                                                                      <  1:106858/140‑1 (MQ=255)                         acacTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGCCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGaa                                                                                                 <  2:456574/140‑1 (MQ=255)                              cAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGCCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAga                                                                                            <  2:418124/140‑1 (MQ=255)                                gCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGCCCTATAACGATAATTTTCGCCTCACAGGAAGCa                                                                                                        >  2:825881/1‑126 (MQ=255)                                gCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGCCCTATAACGATAATTTTCGCCTCACAGGAAGCa                                                                                                        <  1:825881/126‑1 (MQ=255)                                gCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGCCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAgatg                                                                                          >  1:294196/1‑140 (MQ=255)                                gCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGCCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAgatg                                                                                          >  2:480463/1‑140 (MQ=255)                                gCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGCCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAgatg                                                                                          >  1:747775/1‑140 (MQ=255)                                gCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGCCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAgatg                                                                                          >  1:801296/1‑140 (MQ=255)                                  ttATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGCCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAgatgat                                                                                        <  1:873103/140‑1 (MQ=255)                                    aTTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGCCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAgatgatga                                                                                      >  2:605299/1‑140 (MQ=255)                                    aTTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGCCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAgatgatga                                                                                      <  2:153218/140‑1 (MQ=255)                                      tGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGCCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATa                                                                                    >  1:593092/1‑140 (MQ=255)                                           cgcgAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGCCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCcac                                                                               >  1:879923/1‑140 (MQ=255)                                             cgAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGCCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCacac                                                                             <  2:563272/140‑1 (MQ=255)                                             cgAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGCCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCacac                                                                             <  1:480463/140‑1 (MQ=255)                                             cgAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGCCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCacac                                                                             <  1:101656/140‑1 (MQ=255)                                             cgAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGCCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCacac                                                                             <  1:551602/140‑1 (MQ=255)                                                                      tactTTTGCTTATAAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGCCCTATAACGATAATTTTCGCCTCACGGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGc                                                    <  2:388843/140‑1 (MQ=255)                                                                         ttttGCTTATAAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGCCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGt                                                 <  2:813256/140‑1 (MQ=255)                                                                            tGCTTATAAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGCCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTgctgct                                                       >  2:352290/1‑131 (MQ=255)                                                                            tGCTTATAAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGCCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTgctgct                                                       <  1:352290/131‑1 (MQ=255)                                                                            tGCTTATAAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGCCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGc                                              >  2:293630/1‑140 (MQ=255)                                                                            tGCTTATAAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGCCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGc                                              >  1:822178/1‑140 (MQ=255)                                                                                tataAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGCCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCAGCCAGCGTGCTGCTTGCCGTTGCAAAt                                          <  2:747775/140‑1 (MQ=255)                                                                                           ggCCACAGACAGGTAAAAGACGTTTCCCAAACGCCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAgg                                      >  1:751928/1‑133 (MQ=255)                                                                                           ggCCACAGACAGGTAAAAGACGTTTCCCAAACGCCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAgg                                      <  2:751928/133‑1 (MQ=255)                                                                                             ccACAGACAGGTAAAAGACGTTTCCCAAACGCCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTgg                             >  1:765299/1‑140 (MQ=255)                                                                                                 agacagGTAAAAGACGTTTCCCAAACGCCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGcgcg                         <  1:195928/140‑1 (MQ=255)                                                                                                   acagGTAAAAGACGTTTCCCAAACGCCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGAc                       >  1:4247/1‑140 (MQ=255)                                                                                                       gTAAAAGACGTTTCCCAAACGCCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCtt                   >  1:633564/1‑140 (MQ=255)                                                                                                                 tttCCCAAACGCCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGCCGTTc         >  2:980292/1‑140 (MQ=255)                                                                                                                 tttCCCAAACGCCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGCCGTTc         <  2:392094/140‑1 (MQ=255)                                                                                                                        aaCGCCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGCCGTTCAGaaaaa  <  2:241612/140‑1 (MQ=255)                                                                                                                            |                                                                                                                                        CGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGCCGTTCAGAAAAA  >  NC_000913/3418883‑3419141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑